Molecular Evolutional, Geographical, Population Genetical analyses of Wild SilkMoth including Bombyx mandarina distribute in East Asian region

• Project/Area Number: 17405023
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Applied entomology
• Research Institution: University of the Ryukyus
• Principal Investigator: NAKAJIMA Yumiko University of the Ryukyus, Center of Molecular Biosciences, Associate Professor (70244340)
• Co-Investigator(Kenkyū-buntansha): BANNO Yutaka Faculty of Agriculture Kyushu University, Department of Genetic Resource Technology, Associate Professor (50192711) ; YUKUHIRO Keniji University of the Ryukyus, Chief Researcher (50343992) ; ITOH Masanobu Kyoto Institute of Technology, Graduate School of Science and Technology, Associate Professor (60221082) ; KAJIURA Zenta Shinshu University, Faculty of Textile Science and Technology, Associate Professor (10224403) ; SAHARA Ken Hokkaido University, Faculty of Agriculture, Assistant Professor (30241368) ; KUSAKABE Takahiro Kyushu University, Faculty of Agriculture, Associate Professor (30253595)
• Project Period (FY): 2005 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥13,780,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥1,080,000); Fiscal Year 2007: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2005: ¥5,600,000 (Direct Cost: ¥5,600,000)
• Keywords: Bombyx mandarina / Wild Silk moth / mariner-like-elements (MLEs) / Mitochondria Cox1 gene / ALP gene (Alp-m, Alp-s) / Intergenic region / Vitellogenin gene / rDNA region / Cecropia-ITR-MLE / アルカリフォスファターゼ(ALP) / (Alp-m,Alp-s) / ミトコンドリア(mt)cox遺伝子 / クワコ(Bombyx mandarin) / Bmmar6 / L1Bm / アルカリフォスファターゼ(ALP)遺伝子 / ミトコンドリア(mt)cox1遺伝子 / MOKO硫化酵素遺伝子(og) / アルカリ性フォスファターゼ(ALP) / (Alp-mとAlp-s)

Research Abstract

We have analyzed the molecular level of polymorphism in Bombyx mandarina, some other wild silk moth species distribute in wide range of East Asian region by comparing some genes or DNA marker regions among them. B. mori molybdenum cofactor sulfurase gene (og) whose mutants cause translucent skin because of lack or deficiency of accumulation of uric acid under the skin in B. mori. Og genes from Japanese mandarina have wide variety and different from the group of B. mori. We conducted an analysis of mitochondrial coxl gene of B. mori and found low level of sequence diversity relative to that of the og gene. Alkaline phosphatase (Alp) genes are tandemly duplicated in B. mori. The Alp genes (Alp-m, Alp-s) share about 60% identity in the nucleotide sequences, but structure of the intervening region is highly polymorphic with large in/del from strain to strain. In this study we found that the Alp genes were shown to be duplicated in B. mandarina, similarly as in B. mori, in all four gemones. The structure of the intervening region in B. mandarina suggested that the most recent common ancestor of the current silk worm strains and wild B. mandarina existed in China. From the analysis of Cecropia-ITR-MLEs, most of the MLE sequences from B. mandarina in the main island of Japan composed one unique group (Japanese type); on the other hand, the sequences from the Chinese and Korean specimens settled down in another group (Continental type). We have isolated the active type of transposable element (Bmamat1) from the locus of BmTNML of Korean and Japanese mandarina. Vitellogenin genes of B. mandarina are closer to B. mori than other wild silk moth.

Research Products

• [Journal Article] A novel maT-type transposable element. BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6. (2008)
  • Author(s): Kawanishi, Y., Morimoto, M., Bammho Y, Nho, S. K., Maekawa, H., Nakajima, Y.
  • Journal Title: Journal of Insect Biotechnology and Sericology 77(1) Pages: 45-52
  • NAID: 10021220981
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Purification and cDNA Cloning of Vitellogenin of the Wild Silkworm, Saturnia japonica (Lepidoptera: Saturniidae) (2008)
  • Author(s): Meng, Y., Liu, C, Shiomi, K., Nakagaki, M., Banno, Y ., Kajiura, Z. (2008)
  • Journal Title: J. of Insect Biotechnology and Sericology 77 (1) Pages: 35-44
  • NAID: 10021220932
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel war-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6. (2008)
  • Author(s): Kawanishi, Y., Morimoto, M., Banno, Y., Nho, S. K., Maekawa, H., Nakajima, Y.
  • Journal Title: J. of Insect Biotechnology and and Sericology 77 (1) Pages: 45-52
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel maT-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6. (2008)
  • Author(s): Kawanishi, Y., Morimoto, M., Banno, Y., Nho, S. K., Maekawa, H., and Nakajima, Y.
  • Journal Title: Journal of Insect Biotechnology and Sericology 77(1) Pages: 45-52
  • NAID: 10021220981
  • Peer Reviewed
• [Journal Article] Sequence comparison of mariner-like elements among the population of Bombyx mandarina inhabiting China, Korea, and Japan. (2007)
  • Author(s): Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Nho, S. K., Maekawa, H., Nakaiima, Y.
  • Journal Title: J. of Insect Biotechnology and Sericology 76(2) Pages: 79-87
  • NAID: 130004935562
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Sequence comparison of mariner-like elements among the population of Bombyx mandarina inhabiting China, Korea, and Japan. (2007)
  • Author(s): Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Nho, S. K., Maekawa, H., Nakajima, Y.
  • Journal Title: J. of Insect Biotechnology and Sericology 76 (2) Pages: 79-87
  • NAID: 130004935562
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] FISH analysis of the W chromosome in Bombyx mandarina and several other species of Lepidontera by means of B. mori W-BAC probes. 6. Meng, Y., Liu, C, Shiomi, K., Nakagaki, M. (2007)
  • Author(s): Yoshido, A., Yasukochi, Y, Marec, E, Abe, H., Sahara, K.
  • Journal Title: J. of Insect Biotechnology and Sericology 76 (1) Pages: 1-7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] FISH analysis of the W chromosome in Bombyx mandarina and several other species of Lepidoptera by means of B. mori W-BAC probes. (2007)
  • Author(s): Yoshido A, Yasukochi Y, Marec F, Abe H, Sahara K.
  • Journal Title: Journal of Insect Biotechnology and Sericology 76(1) Pages: 1-7
  • NAID: 10018742816
  • Peer Reviewed
• [Journal Article] Prevalence of full-size P and KP elements in North American populations of Drosophila melanogaster. (2007)
  • Author(s): Itoh, M., Takuchi, N., Yamaguchi, M., Yamamoto, M-T., Boussy, I.A.
  • Journal Title: Genetica (in press)
• [Journal Article] Sequence Comparison of mariner-like elements among the populations of Bombyx mandarina inhabiting China, Korea and Japan. (2007)
  • Author(s): Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Nho, S.K., Maekawa, H., Nakajima,Y.
  • Journal Title: J.Insect Biotechnology and Sericology 76(2)(in press)
  • NAID: 130004935562
• [Journal Article] The W chromosome detection in several lepidopteran species by genomic in situ hybridization (GISH). (2006)
  • Author(s): Yoshido, A., Yamada, Y, Sahara, K.
  • Journal Title: J. of Insect Biotechnology and Sericology 75(3) Pages: 147-151
  • NAID: 10020516441
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Genetic variations in the vitellogenin of Japanese populations of the wild silkworm Bombyx mandarina. (2006)
  • Author(s): Meng, Y., Liu, C, Shiomi, K., Nakagaki, M., Banno, Y., Kajiura, Z.
  • Journal Title: J. of Insect Biotechnology and Sericology 75(3) Pages: 127-134
  • NAID: 10020516398
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Method for rapid distinction of Bombyx mandarina (Japan) from B. mamdarina (China) based on rDNA sequence difference. (2006)
  • Author(s): Kawanishi, Y., Banno, Y., Fujimoto, H., Nho, S. K., Tu, Z., Mita, K., Tshuchida, K., Takada, N., Maekawa, H., and Nakajima, Y.
  • Journal Title: Journal of Insect Biotechnology and Sericology 77(2)(in press)
  • Peer Reviewed
• [Journal Article] Genetic variation in the vitellogenin of Japanese population of the wild silkworm,Bombyx mandarina. (2006)
  • Author(s): Meng, Y., Liu, C., Kunihiro, S., Nakagaki, M., Banno, Y., Kajiura,Z.
  • Journal Title: J.Insect Biotechnology and Sericology 75(3) Pages: 127-134
  • NAID: 10020516398
• [Journal Article] Interaction of Subtilisin BPN' and recombinant Fungal Protease Inhibitor F from silkworm with substituted P1 site residues. (2006)
  • Author(s): Taniguchi, M., Atiwetin, P., Hirai, T., Itoh, M., Harada, S., Hara, S., Kamei,K.
  • Journal Title: Biosci.Biotechnol.Biochem. 70 Pages: 1262-1264
• [Journal Article] Transposable hobo elements in natural populations of Drosophila melanogaster in Japan. (2006)
  • Author(s): Kikuno, K., Tanaka, K., Itoh, M., Tanaka, Y., Boussy, I.A., Gamo,S.
  • Journal Title: Heredity 96 Pages: 426-433
• [Journal Article] Micropylar structure of chorion of the female sterile mutation, bd, in Bombyx mori. (2006)
  • Author(s): Kawaguchi Y., Kusakabe T., Lee J.M., Nakajima Y., Koga K.
  • Journal Title: Journal of Insect Biotechnology and Sericology 75(1) Pages: 9-14
  • NAID: 10017316105
• [Journal Article] A novel indicator for radiation sensitivity using the wing size reduction of Bombyx mori pupae caused by γ-ray irradiation. (2006)
  • Author(s): Takada N., Yamauchi E., Fujimoto H., Banno Y., Tsuchida K., Hashido K., Nakajima Y., Tu Z., Takahashi M., Fujii H., Fugo H., Maekawa H.
  • Journal Title: Journal of Insect Biotechnology and Sericology 75(1)(In press)
  • NAID: 10020516481
• [Journal Article] Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG) n telomeric probe in some species of Lepidoptera. (2005)
  • Author(s): Yoshido, A., Marec, F., Sahara, K.
  • Journal Title: Chromosoma 114 Pages: 193-202
  • NAID: 120000956785
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sequence Comparison of mariner-like-elements and inserted sequences into them among Bombyx mandarina inhabiting in China, Korea and Japan. (2005)
  • Author(s): Kawanishi.Y., Takaishi R., Banno Y., Fujimoto H., Maekawa H., Nakajima, Y.
  • Journal Title: Proceedings of 5^<th> Asia-Pacific Congress of Entomology Pages: 60-60
• [Journal Article] Vitellogenin gene organization of Antheraea yamamai and promoter activity analysis.
  • Author(s): Meng, Y., Liu, C, Zhao, A., Shiomi, K., Nakagaki, M., Kajiura, Z.
  • Journal Title: Int. J. of Wild Silkmoth & Silk. 11 Pages: 29-40
  • NAID: 10022022566
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] mariner-like element(MLE)に挿入されたレトロトランスポゾンL1Bm,MLE Bmmar6領域,およびrDNAクラスターの特異性に基づく,クワコ集団間の比較,およびカイコとの分岐経路の考察 (2007)
  • Author(s): 川西祐一・伴野豊・盧時甲・間野修平・前川秀彰・中島裕美子
  • Organizer: BMB2007(第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会)
  • Place of Presentation: 横浜:パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] Sequence comparison of the mariner-Iike-elements (MLEs)and their inserts among the populations of some species inhabiting in East Asian region. (2007)
  • Author(s): Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Nho, S. K., Tanaka, C, Tanaka, J., Maekawa, H., Nakajima, Y.
  • Organizer: International Congress of Insect Biotechnologyand Industry (ICIBI)
  • Place of Presentation: Daegu, 韓国, EXCO
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Sequence comparison of the mariner-like-elements (MLEs) and their inserts among the populations of some species, inhabiting in East Asian region. (Invited speaker) (2007)
  • Author(s): Kawanishi, Y., Takaishi, R.,Banno, Y, Fujimoto, H., Nho, S. K., Tanaka, C, Tanaka, J., Maekawa, H., Nakajima, Y.
  • Organizer: International Congress of Insect Biotechnology andIndustry (ICIBI)
  • Place of Presentation: EXCO, Daegu, Republic of Korea.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] BAC-FISH analysis of Bombyx mori chromosomes. (Invited speaker) (2006)
  • Author(s): Sahara, K., Yoshido, A, Yasukochi, Y.
  • Organizer: International symposium on insect genetics and genomics
  • Place of Presentation: Centre for DNA fingerprinting and diagnostics, Hyderabad, India..
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Sequence Comparison of mariner-like-elements and inserted sequences into them among Bombyx mandarina inhabiting in China, Korea and Japan. (Invited speaker) (2005)
  • Author(s): Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Maekawa, H., Nakaiima. Y.
  • Organizer: 5th Asia-Pacific Congress of Entomology
  • Place of Presentation: Cheju, Republic of Korea.
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 転移性配列を利用した亜熱帯域生物の集団遺伝学的解析(琉球大学21世紀COEプログラム編集委員会編)「美ら島の自然史-サンゴ礁島嶼系の生物多様性」 (2006)
  • Author(s): 中島裕美子
  • Publisher: 東海大学出版会
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 美ら島の自然史-サンゴ礁島嶼系の生物多様性、琉球大学21世紀COE編集委員会(編) -転移性配列を利用した亜熱帯域生物の集団遺伝学的解析- (2006)
  • Author(s): 中島 裕美子
  • Publisher: 東海大学出版会