Study on Safety of Xenotransplantation: Characterization of Porcine Endogenous Retrovirus of Clawn Miniature Swine
| Project/Area Number |
17500288
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kagoshima University |
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Principal Investigator |
FUJIYOSHI Toshinobu Kagoshima University, Frontier Science Research Center, Associate Professor (50173480)
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| Co-Investigator(Kenkyū-buntansha) |
SONSHIN Takao Kagoshima University, Frontier Science Research Center, Professor (80171411)
MATSUBARA Shu-Ichiro Kagoshima University, Frontier Science Research Center, Associate Professor (60199841)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Xenotransplantation / PERV / Clawn miniature swine / Biohazard |
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| Research Abstract |
Miniature swine are considered the most likely animals for xenoplantaion to human. However, their genome contains porcine endogenous retrovirus (PERV) capable of infecting human cells in vitro. In order to expole a potential use of miniature swine foe xenotransplantation, a new strain of Clawn miniature swine was developed in Kagoshima, Japan and investgatied for the presence of PERV genome. Peripheral blood mononuclear cells (PBMC) DNA of seven individual Clawn miniature swine was analyzed by the specific polymerase chain reaction (PCR) method, using the known primer for PERV gag, PERV pol, PERV-A env, PERV-B env, and PERV-C env. The PBMC DNA of three Large Yokshire pigs and three Landrace pigs as well as eight Kurobuta Berksire pigs, were examined for comparison. All of the tested samples were PERV-A, B, and C positive. In the present study, we demonstrated the presense pf PERV-A, B, and C
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Establishment of cell lines that exhibit pluriopotency from miniature swine periodontal ligaments2007
Author(s)
Ibi M, Ishisaki A, Yamamaoto M, Wada S, Kozakai T, Nakashma A, Iida J, Takao S, Izumi Y, Yokoyma A, Tmnura M
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Journal Title
Arch. Oral Biology 52
Pages: 1002-1008
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Establishment of cell lines that exhibit pluriopotency from miniature swine periodontal ligaments2007
Author(s)
Ibi M, Ishisaki A, Yamamaoto M, Wada S, Kozakai T, Nakashima A, Iida J, Takao S, Izumi Y, Yokoyama A, Tamura M
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Journal Title
Arch. Oral Biology 52
Pages: 1002-1008
Description
「研究成果報告書概要(欧文)」より
Related Report
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