| Project/Area Number |
17500427
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Tottori University |
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Principal Investigator |
IMOTO Toshiaki Tottori University, Faculty of Medicine, Associate Prof., 医学部, 助教授 (10109639)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Tatsuo Tottori University, Faculty of Medicine, Professor, 医学部, 教授 (60182929)
MIYOSHI Michio Tottori University, Faculty of Medicine, Research Assoc., 医学部, 助手 (20093627)
TANAKA Toshiyuki Tottori University, Faculty of Medicine, Associate Prof., 医学部, 助教授 (50116349)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | taste / stress / licking / rat / rare metal ions / two bottle choice method |
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| Research Abstract |
Present studies were performed to clarify the effect of various stresses on taste preference and/or sensitivity of rats based on the observation of taste behavioral changes, which were assessed by either the conventional two bottle choice method or counting the number of licking for various taste solution per 10 sec. Plasma levels of heavy metal ions such as Fe, Cu and Zn were also compared before and after the stress with each other.
(1) Effect of lipopolysaccharide (LPS) on the taste responses.
The licking behavior for five basic taste stimuli were observed before, during and after the intravenous injection of LPS (100μg/kg of body weight, two times with one week interval). There have been observed no significant behavioral changes.
(2) Effect of the restraint stress on the behavioral taste responses.
Taste preference assessed by two bottle choice method was measured before, during and after the experimental schedule of loading the restraint stress (10:00 - 15:00, every day for four weeks). Interestingly, it was noticed before the beginning of the experiment that significant difference exists in sensitivity for bitter taste, that is, one group rejecting 0.3 M sucrose mixed with 0.1 mM quinine hydorochloride (Q-HCL), while other still showing strong preference to the same solution. During the stress, significant increase of preference for sucrose and NaCl mixed with Q-HCl were observed in the former group, while the intake of umami solution of mono-sodium glutamate was significantly decreased during stress in both group The results were discussed considering the change of plasma levels of heavy metal ions together.
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