Effect of smoking on DNA damage and chromosome aberration in alveolar macrophage
| Project/Area Number |
17500488
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
TAKEUCHI Minoru Kyoto Sangyo University, Faculty of Engineering, Professor (70257773)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,750,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Smoking / Alveolar macrophage / DNA damage / Chromosome aberration / Genetic aberration / 遺伝子損傷 / タバコ喫煙 |
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| Research Abstract |
We examined concerning the effect of the smoking on the gene (DNA) damage and the chromosome aberration of the alveolar macrophage from 02- production of the alveolar macrophage being strengthened by smoking. The DNA damage and chromosome aberration of the alveolar macrophage was caused by smoking. The chromosome aberration of was presented in all the chromosomes in alveolar macrophage. We examined about 43000 genes from unusual points of chromosomes, the number of total genes which aberration points were detected was 280 genes compared with non-smoking, and the number of genes which increased more than 2 times expression was 127, however, genes expressions which decreased more than 2 times were 153 compared with non-smoking. Specially, the gene expression increased by smoking was chemokine genes, conversely the gene expressions decreased by smoking were adhesion molecules and the prostacyclin synsetiyze enzyme. Moreover, increase in 8-OHdG which is closely associated with carcinogenesis was detected in DNA of lung tissue by smoking, the expression of 8-OHdG was recognized on mucous epithelial cells in bronchiole. These results suggested that the mechanism of inhibition of immune functions in alveolar macrophage by smoking was associated with caused DNA damage by generated OH through H_2O_2 from O_2^-, and its results was occurred inhibition of expression of antigen presenting molecule and inhibition of cytokine production. It was suggested that increase in the generation of 8-OHdG by excessively reactive oxygen species (ROS) from alveolar macrophage by smoking and inhibition of immune function were related to incidence of lung cancer. Furthermore, the repression of immune function of alveolar macrophage by cigarette smoke influenced the early stage of B cell, and its result inhibited antibody production, and these results may be present the possibility to increase for infection.
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Report
(4 results)
Research Products
(69 results)
