| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
The results of my studies about the mechanism of the release of abnormal bases by synthesis of cellular oxidative radical reaction were describe, during from April of 2005 to March of 2006, as follows.
1. Identification of oxidative abnormal bases by cellular oxidative radical reaction.
It was found that abnormal bases from Bacillus subtilis and Geobacillus were identified as a analogues of 2-OH-dATP by purification and analysis by using the NMR. Moreover, Sphingomonad group (including Sphingobium amiense, Sphingobium yanoikuyae, Sphingomonas cloacae had main abnormal bases such as 8-oxo-dGTP.
2. Analysis of the mechanism of the release of abnormal bases.
Five genes for cellular oxidative radical reaction (including sod(superooxide-dismutase), shpC(alkylhydroxyreductase), mrgA(DNA binding protein), katA(catalase), fur (ferric uptake regulator) )were cloned from the chromosome of Bacillus subtilis.
3. Analysis of the MutT homologue from Sphingomonas group.
Sphingomonad group (including Sphingobium amiense, Sphingobium yanoikuyae, Sphingomonas cloacae had some homologues genes of the muTgene (degradation of the main abnormal bases such as 8-oxo-dGTP).
4. Isolation of three plasmid harboring the enzyme of MutT homologue from Sphingomonas group.
Three plasmids were isolated from Sphingobium amiense and Sphingobium yanoikuyae and named pAMI-1, pYAN-1 and p YAN-2. The nucleotide sequence of these plasmid were completely determined.
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