Development and application of the rapid cultivation and isolation method of petroleum-degrading bacteria by enrichment culture using soils
| Project/Area Number |
17510061
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Hokkaido University |
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Principal Investigator |
OKUYAMA Hidetoshi Hokkaido University, Faculty of Environmental Earth Science, associate Professor, 大学院地球環境科学研究院, 助教授 (90125295)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Petroleum-degrading bacteria / Soil DNA / Naphthene degrader / Isoalkane degrader / Crude oil-degrading bacteria / Autochthonous bioaugmentation / Soil cultivation |
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| Research Abstract |
1.The bioaugmentation method (BA) is a useful technology for decontamination of petroleum-contaminated soils. For this purpose it is necessary to isolate petroleum-degrading bacteria with high activity and to apply such bacteria to soils. To promptly respond to oil spills from oil tankers and tanks isolation and characterization of oil-degrading bacteria should be also prompt. In this study I aimed to develop prompt isolation procedures of petroleum-degrading bacteria.
2.Isolation of petroleum-degrading bacteria from soils (petroleum-contaminated and petroleum-non-contaminated soils) was achieved by cultivating them with soil extracts.
3.Petroleum-degrading isolates from soils are generally able to efficiently degrade petroleum under liquid cultivation conditions. However, most of them did not exhibit significant degrading activity of petroleum in soils.
4.One strain from petroleum-non-contaminated paddy soils was isolated.
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Report
(3 results)
Research Products
(14 results)
