| Project/Area Number |
17510158
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
基礎ゲノム科学
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
HORIE Kyoji Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (30333446)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
|
|---|
| Keywords | retrotoransposon / IAP / mouse / genome / expression |
|---|
| Research Abstract |
Using the intracisternal A particle (IAP) element as a model system, we conducted mouse experiments for studying the interaction between retrotransposons and the genome. We generated transgenic mice bearing IAP elements that were newly isolated by us from mouse acute myeloid leukemia cells. These mice did not show any overt phenotype in tumorigenesis. Analysis of IAP-retrotransposition events in mouse tissues revealed that retrotransposition efficiency was significantly low in live mice compared with cultured cells. This result implicates the presence of machinery for silencing the activity of IAP transgenes. We therefore mated IAP transgenic mice with other mutant mice deficient in genome defense systems, and obtained preliminary data of elevation of retrotransposition efficiency. This observation suggests that our IAP transgenic mice may serve as a model system for the study of genome defense systems against retrotransposon invasion. We also found active transcription from IAP elements widely distributed across the mouse genome, and proposed its influence on the shaping of mouse transcriptome. Furthermore, distribution of IAP elements in the genome was significantly different between mouse strains, suggesting possible influence of IAP elements on the divergence of genetic traits.
|
