Hibernation-associated gene expression regulated by epigenetic modifications
| Project/Area Number |
17510160
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Kitasato University |
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Principal Investigator |
TAKAMATSU Nobuhiko Kitasato University, School of Science, Assistant Professor, 理学部, 助教授 (40206876)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Michihiko Kitasato University, School of Science, Lecturer, 理学部, 講師 (90240994)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | gene expression / transcriptional regulation / histone modification / chromatin immunoprecipitation / hibernation / liver / ヒストン修飾 / クロマチン免疫沈降法 / CpG配列 / メチル化 / USF |
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| Research Abstract |
The chipmunk hibernation-specific proteins HP-20, HP-25 and HP-27 were identified as components of a 140 kDa complex that decreases drastically in the blood during hibernation. The hibernation-specific genes encoding HP-20, HP-25 and HP-27 are expressed specifically in the liver and are downregulated during hibernation. The liver-specific transcription of the HP-20 and HP-25 genes are activated by HNF-1 and HNF-4, respectively. In this study, we first investigated other transcription factors involved in the liver-specific transcription of the hibernation-specific genes, and to date, revealed that the HP-20, HP-25 and HP-27 genes are activated by HNF-1, HNF-4 and USF, by HNF-4 and USF, and by USF, respectively. Next, to analyze whether these transcription factors are involved in the transcriptional regulation of the HP-20, HP-25 and HP-27 genes in vivo, we performed chromatin immunoprecipitation (ChIP) using anti-HNF-1, anti-HNF-4 and anti-USF antibodies. ChIP revealed that these transcription factors bound to the promoter region of the respective hibernation-specific genes in the liver of nonhibernating chipmunk, indicating that these transcription factors are involved in the transcriptional regulation of the hibernation-specific genes in vivo. ChIP also revealed that the transcription factors bound to the hibernation-specific gene promoters even in the liver of hibernating chipmunks in which their transcription is repressed. We then analyzed whether epigenetic modifications are involved in the hibernation-associated transcriptional regulation. ChIP revealed that histone H3 bound to the promoter regions of the hibernation-specific genes was acetylated at lysine 14 in nonhibernating chipmunks, but not in hibernating chipmunks. Taken together, these results indicate that the hibernation-associated transcription of the chipmunk hibernation-specific genes is regulated mainly by epigenetic modification including histone acetylation.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] USF is involved in the transcriptional regulation of the chipmunk HP-25 gene.2007
Author(s)
Tsukamoto, D., Fujii, G., Kondo, N., Ito, M., Shiba, T., Takamatsu, N.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] CpG methylation at the USF-binding site is important for the liver-specific transcription of the chipmunk HP-27 gene.2006
Author(s)
Fujii, G., Nakamura, Y., Tsukamoto, D., Ito, M., Shiba, T., Takamatsu, N.
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Journal Title
Biochem.J. 395
Pages: 203-209
Description
「研究成果報告書概要(欧文)」より
Related Report
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