Gene expression profile during the life cycle of the ascidians
| Project/Area Number |
17510162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Hokkaido University |
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Principal Investigator |
AZUMI Kaoru Hokkaido University, Faculty of Pharmaceutical Sciences, Instructor, 大学院薬学研究院, 助手 (90221720)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Ascidian / Ciona intestinalis / Gene expression / DNA microarray / Clustering / Development / Ageing / ライフサイクル / 組織 / ダイオキシン |
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| Research Abstract |
Recent whole-genome studies and in-depth expressed sequence tag (EST) analyses have identified most of the developmentally relevant genes in the urochordate, Ciona intestinalis. In this study, we made use of a large-scale oligo-DNA microarray to further investigate and identify genes with specific or correlated expression profiles, and we report global gene expression profiles for about 66% of all the C. intestinalis genes that are expressed during its life cycle. We succeeded in categorizing the dataset into 5 large clusters and 49 sub-clusters based on the expression profile of each gene. This revealed the higher order of gene expression profiles during the developmental and ageing stages. Furthermore, a combined analysis of microarray data with the EST database revealed the gene groups that were expressed at a specific stage or in a specific organ of the adult. This study provides insights into the complex structure of ascidian gene expression, identifies co-expressed gene groups and marker genes, and makes predictions for the biological roles of many uncharacterized genes. This large-scale oligo-DNA microarray for C. intestinalis should facilitate the understanding of global gene expression and gene networks during the development and ageing of a basal chordate.
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Report
(3 results)
Research Products
(24 results)
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[Book] バイオとナノの融合I2007
Author(s)
安住 薫
Total Pages
363
Publisher
北海道大学出版会
Description
「研究成果報告書概要(和文)」より
Related Report
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[Book] 環境修復の技術と科学2007
Author(s)
安住 薫
Total Pages
256
Publisher
北海道大学出版会
Description
「研究成果報告書概要(和文)」より
Related Report
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