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Elucidation of the most important muse of lower hatching rate in case of transplanted fertilized eggs in the wild loggerhead sea turtle

Research Project

Project/Area Number 17510195
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Resource conservation science
Research InstitutionGifu University

Principal Investigator

DOI Osamu  Gifu University, Faculty of Applied Biological Sciences, Professor (60180212)

Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsloggerhead sea turtle / fertilized egg / sex steroid hormone / incubation period / clouded eggshell / 移植
Research Abstract

In the loggerhead sea turtle (Caretta earetta), the embryo adhesion to eggshell membrane and a part of the clouded eggshell were driven from the top of fertilized eggs during incubation. However, embryos did not always adhere to the top of eggshells under the normal incubation condition, and some embryo in 15-day incubation was observed to develop in the bottom of eggs. From this result, it was found that sea turtle embryos do not necessarily become attached to the inner surface on top of eggshell. Moreover, sea turtle eggs are sensitive to turning shortly after laying, and fertilized eggs of the nest that must be relocated under a bad condition for incubation should be moved within 12 hours of laying or after about 40 days of incubation time. With data logger, the temperature and moisture of the sand at wild beach were measured and degree-days was calculated. The sand temperature and moisture was closely related to the precipitation in nature, and total temperature must be over 1800 degrees for hatching. On the other hand, serum estradiol-17β (E2) concentration in female and testosterone (T) concentration in male increased when water temperature declined in captivity. That is, serum sex steroid hormone (except for serum progesterone) increased at September to February (the increased period in water temperature), and decreased from May to July (the decreased period). Female serum progesterone concentration increased at February to May just before the onset of laying in nature. In this study, the timing and method of transplantation for sea turtle egg in circumstances where eggs can not be expected to hatching after laying, and endocrinological and reproductive physiology of the loggerhead sea turtle were able to be clarified. These results of this study may be helpful in protecting the loggerhead sea turtle from extermination at Japanese beach.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (7 results)

All 2007 2006 2005

All Journal Article (1 results) Presentation (6 results)

  • [Journal Article] アカウミガメにおける移植した受精卵の孵化率低下原因の解明と産卵生理に関する研究2006

    • Author(s)
      土井 守, 寺嶋淳美, 加藤隆司, 福岡敏夫, 市川憲平, 増田 修, 後藤 清, 松沢慶将
    • Journal Title

      第14回希少動物人工繁殖研究会議資料集

      Pages: 25-26

    • Related Report
      2006 Annual Research Report
  • [Presentation] アカウミガメの胚発生の進行と産卵生理に関する研究2007

    • Author(s)
      田中浩之, 寺嶋淳美, 加藤隆司, 福岡敏夫, 市川憲平, 増田 修, 後藤清, 松沢慶将, 楠田哲士, 土井 守
    • Organizer
      第15回希少動物人工繁殖研究会議
    • Place of Presentation
      神戸市立王子動物園
    • Year and Date
      2007-06-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Ontogenetic and endocrinological studies in the loggerhead sea turtle2007

    • Author(s)
      Tanaka, H., Terashima, A., Kato, T., Fukuoka, T., Ichikawa, K., Masuda, O., Goto, K., Matsuzawa, K., Kusuda, S., Doi, O
    • Organizer
      Proceeding of the 15th Annual Meeting in Japanese Study Group on Artificial Reproduction of Endangered Animals
    • Year and Date
      2007-06-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Elucidation of the most important cause of lower hatching rate in case of transplanted fertilized eggs and endocrinological study in the loggerhead sea turtle2006

    • Author(s)
      Doi, O., Terashima, A., Kato, T., Fukuoka, T., Ichikawa, K., Masuda, O., Goto, K., Matsuzawa, K
    • Organizer
      Proceedings of the 14th Annual Meeting in Japanese Study Group on Artificial Reproduction of Endangered Animals
    • Year and Date
      2006-06-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アカウミガメにおける移植した受精卵の孵化率低下原因の解明と産卵生理に関する研究2005

    • Author(s)
      土井 守, 寺嶋淳美, 加藤隆司, 福岡敏夫, 市川憲平, 増田 修, 後藤 清, 松沢慶将
    • Organizer
      第14回希少動物人工繁殖研究会議
    • Place of Presentation
      大阪市天王寺動物園
    • Year and Date
      2005-06-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] アカウミガメの卵殻構造の組織学的変化と卵殻内培養法による胚発生に関する研究2005

    • Author(s)
      土井 守, 佐藤宏佳, 杉山智香, 亀崎直樹, 松沢慶将, 後藤 清, 小久保信一, 福岡敏夫, 大池辰也, 黒柳賢治, 栃本武良, 湯浅義明, 市川憲平, 三木 徹, 増田 修, 津田英治, 清水邦一, 脇本久義, 多田雄作
    • Organizer
      第13回希少動物人工繁殖研究会議
    • Place of Presentation
      東京都多摩動物公園
    • Year and Date
      2005-06-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Ultrastructural histological study of eggshell and development in culture of the embryo in the loggerhead sea turtle2005

    • Author(s)
      Doi, O., Sato, H., Sugiyama, T., Kamezaki, N., Matsuzawa, K., Goto, K., Kokubo, S., Fukuoka, T., Oike, T., Kuroyanagi, K., Tochimoto, T., Yuasa, Y., Ichikawa, K., Mild, T., Masuda, O., Tsuda, E., Shimizu, K., Wakimoto, H., Tada, Y
    • Organizer
      Proceedings of the 13th Annual Meeting in Japanese Study Group on Artificial Reproduction of Endangered Animals
    • Year and Date
      2005-06-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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