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A genetic analysis on a novel regulatory mechanism to induce cell death in response to perturbation of dynamic instability of microtubules

Research Project

Project/Area Number 17570006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionKyoto Institute of Technology

Principal Investigator

INOUE Yoshihiro  Kyoto Institute of Technology, Drosophila Genetic Resource Center, Lecturer, ショウジョウバエ遺伝資源センター, 講師 (90201938)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Masamitsu  Kyoto Institute of Technology, Graduate School of Science and Technology, Professor, 工芸科学研究科, 教授 (00182460)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsmicrotubules / Drosophila / apoptosis / Orbit / dynamic instability
Research Abstract

Microtubules are indeed involved in many cellular phenomena. It has been shown that dynamic instability of microtubules is especially important for regulation of some cellular events. The Orbit/CLASP, a conserved microtubule-associated protein enriched at plus-ends of microtubules plays an essential role in regulation of the dynamic instability. Loss-of-function mutations of the orbit in Drosophila increase instability of microtubules. Conversely, over-expression of the gene in imaginal discs using GAL4-UAS system causes excessive stabilization of microtubules and consequently a formation of microtubule bundles that are never observed in normal cells at interphase. The over-expression of the orbit in eye disc cells after morphogenic furrows accumulates metaphase cells, suggesting it activates the microtubule assembly checkpoint in mitosis. We found that the over-expression eventually induces apoptosis dependent on Caspase 3 at post-mitotic stage in the eye discs. Knockdown of the gene also causes induction of the Caspase 3 in wing discs. This type of apoptosis cannot be observed in non-dividing cells such as larval salivary gland cells with over-expression of the orbit. The induction of apoptosis is required for a proper function of mitotic checkpoint genes and p53. Our data should suggest that cells with abnormal microtubules deviated from proper range of the dynamic instability could be recognized during mitosis and could be eliminated by the apoptosis from proliferating tissues.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (33 results)

All 2007 2006 2005 Other

All Journal Article (31 results) Book (2 results)

  • [Journal Article] Transcriptional regulation of the Drosophila rfc1 gene by the DRE-DREF pathway.2007

    • Author(s)
      Tsuchiya, A., Inoue, Y.H., Ida, H., Kawase, Y., Okudaira, K., Ohno, K., Yoshida, H., Yamaguchi, M.
    • Journal Title

      FEBS J. 274

      Pages: 1818-1832

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Complex interference in the eye developmental pathway by Drosophila NF-YA.2007

    • Author(s)
      Yoshioka, Y., Suyari, O., Yamada M., Ohno, K., Hayashi, Y., Yamaguchi, M.
    • Journal Title

      Genesis 45

      Pages: 21-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system.2007

    • Author(s)
      Kim, Y.S, Shin, M.J., Yang, D.J., Yamaguchi, M., Park, S.Y., Yoo, M.A.
    • Journal Title

      Genes to Cells. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prevalence of full-size P and KP elements in North America populations of Drosophila melanogaster.2007

    • Author(s)
      Itoh, M., Takeuchi, N., Yamaguchi, M., Yamamoto, M., Boussy, I., A.
    • Journal Title

      Genetica (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a.2007

    • Author(s)
      Nishida, M., Kato, M., Kato, Y., Sasai, N., Ueda, J., Tachibana, M., Shinkai, Y., Yamaguchi, M.
    • Journal Title

      Genes to Cells. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of the Drosophila rfcl gene by the DRE-DREF pathway2007

    • Author(s)
      Tsuchiya, A., Inoue, Y.H., Ida, H., Kawase, Y., Okudaira, K., Ohno, K., Yoshida, H, Yamaguchi, M.
    • Journal Title

      FEBS J. 274

      Pages: 1818-1832

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Complex interference in the eye developmental pathway by Drosophila NF-YA.2007

    • Author(s)
      Yoshioka, Y, Suyari, O., Yamada, M., Ohno, K., Hayashi, Y., Yamaguchi, M.
    • Journal Title

      Genesis. 45

      Pages: 21-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Chromosome dynamics regulated by Orbit/CLASP protein family. (in Japanese)2007

    • Author(s)
      Ando, I., Suzuki.T, Inoue, Y.H.
    • Journal Title

      NTS Press

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of the Drosophila rfcl gene by the DRE-DREF pathway.2007

    • Author(s)
      Tsuchiya, A., Inoue, Y.H., Ida, H., Kawase, Y., Okudaira, K., Ohno, K., Yoshida, H., Yamaguchi, M.
    • Journal Title

      FEBS J. 274

      Pages: 1818-1832

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Complex interference in the eye developmental pathway by Drosophila NF-YA.2007

    • Author(s)
      Yoshioka, Y., Suyari, O., Yamada, M., Ohno, K., Hayashi, Y., Yamaguchi, M.
    • Journal Title

      Genesis 45

      Pages: 21-31

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system.2007

    • Author(s)
      Kim, Y.S, Shin, M.J., Yang, D.J., Yamaguchi, M., Park, S.Y., Yoo, M.A.
    • Journal Title

      Genes to Cells (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a.2007

    • Author(s)
      Nishida, M., Kato, M., Kato, Y., Sasai, N., Ueda, J., Tachibana, M., Shinkai, Y., Yamaguchi, M.
    • Journal Title

      Genes to Cells (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Drosophila Damaged DNA Binding Protein 1 contributes to genome stability in somatic cells.2006

    • Author(s)
      Shimanouchi, K., Takata, K., Yamaguchi, M., Murakami, S., Ishikawa, G., Takeuchi, R., Kanai, Y., Ruike, T., Nakamura, R., Abe, Y., Sakaguchi, K.
    • Journal Title

      J. Biochem. 139

      Pages: 51-58

    • NAID

      10018848289

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Antagonistic regulation of the Drosophila PCNA gene promoter by DREF and Cut.2006

    • Author(s)
      Seto, H., Hayashi, Y., Kwon, E., Taguchi, O., Yamaguchi, M.
    • Journal Title

      Genes to Cells 11

      Pages: 499-512

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Genetic link between β-sarcoglycan and the Egfr signaling pathway.2006

    • Author(s)
      Hashimoto, R., Yamaguchi, M.
    • Journal Title

      Biochem. Biophys. Res. Commun. 348

      Pages: 212-221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Drosophila Distal-less negatively regulates dDREF by inhibiting its DNA binding activity.2006

    • Author(s)
      Hayashi, Y., Kato, M., Seto, H., Yamaguchi, M.
    • Journal Title

      Biochim. Biophys. Acta. 1759

      Pages: 359-366

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Identification of the Drosophila skpA gene as a novel target of the transcription factor DREF.2006

    • Author(s)
      Thao, D.T.P., Ida, H., Yoshida, H., Yamaguchi, M.
    • Journal Title

      Exp. Cell Res. 312

      Pages: 3641-3650

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Dynamic changes in the subcellular localization of Drosophila β-Sarcoglycan during the cell cycle.2006

    • Author(s)
      Hashimoto, R., Yamaguchi, M.
    • Journal Title

      Cell Struct. Funct. 31

      Pages: 173-180

    • NAID

      40015510810

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Drosophila Damaged DNA Binding Protein 1 contributes to genome stability in somatic cells.2006

    • Author(s)
      Shimanouchi, K., Takata, K., Yamaguchi, M., Murakami, S., Ishikawa, G, Takeuchi, R., Kanai, Y, Ruike, T, Nakamura, R Abe, Y.Sakaguchi, K.
    • Journal Title

      J.Biochem. 139

      Pages: 51-58

    • NAID

      10018848289

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic link between p-sarcoglycan and the Egfr signaling pathway.2006

    • Author(s)
      Hashimoto, R., Yamaguchi, M.
    • Journal Title

      Biochem.Biophys.Res.Commun. 348

      Pages: 212-221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Drosophila Distal-less negatively regulates dDREF by inhibiting its DNA binding activity.2006

    • Author(s)
      Hayashi, Y., Kato, M., Seto, H.Yamaguchi, M.
    • Journal Title

      Biochim.Biophys.Acta. 1759

      Pages: 359-366

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dynamic changes in the subcellular localization of Drosophila β-Sarcoglycan during the cell cycle.2006

    • Author(s)
      Hashimoto, R., Yamaguchi, M.
    • Journal Title

      Cell Struct.Funct. 31

      Pages: 173-180

    • NAID

      40015510810

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetic link between □-sarcoglycan and the Egfr signaling pathway.2006

    • Author(s)
      Hashimoto, R., Yamaguchi, M.
    • Journal Title

      Biochem. Biophys. Res. Commun. 348

      Pages: 212-221

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Dynamic changes in the subcellular localization of Drosophila □-Sarcoglycan during the cell cycle.2006

    • Author(s)
      Hashimoto, R., Yamaguchi, M.
    • Journal Title

      Cell Struct. Funct. 31

      Pages: 173-180

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Microtubule bundle formation and cell death induced by the human Orbit/CLASP N-terminal fragment.2005

    • Author(s)
      Aonuma, M., Miyamoto, M., Inoue, Y.H., et al.
    • Journal Title

      Cell Struct.Funct. 20

      Pages: 7-13

    • Related Report
      2005 Annual Research Report
  • [Journal Article] parva germina, a Gene Involved in germ cell maintenance during male and female Drosophila gametogenesis.2005

    • Author(s)
      Giovanna, M., Inoue, Y.H., Callaini, G
    • Journal Title

      Dev.Dynamics 232

      Pages: 835-844

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Antagonistic regulation of the Drosophila PCNA gene promoter by DREF and Cut.

    • Author(s)
      Seto, H., Hayashi, Y, Kwon, E., Taguchi, O., Yamaguchi, M.
    • Journal Title

      Genes to Cells 11

      Pages: 499-512

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of the Drosophila skpA gene as a novel target of the transcription factor DREF.

    • Author(s)
      Thao, D.T.P., Ida, H., Yoshida, H.Yamaguchi, M.
    • Journal Title

      Exp.Cell Res. 312

      Pages: 3641-3650

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system.

    • Author(s)
      Kim, Y.S, Shin, M.J., Yang, D.J., Yamaguchi, M., Park, S.Y., Yoo, M.A.
    • Journal Title

      Genes to Cells (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Prevalence of full-size P and KP elements in North America populations of Drosophila melanogaster.

    • Author(s)
      Itoh, M., Takeuchi, N., Yamaguchi, M., Yamamoto, M., Boussy, I., A.
    • Journal Title

      Genetica (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a.

    • Author(s)
      Nishida, M., Kato, M., Kato, Y., Sasai, N., Ueda, J., Tachibana, M., Shinkai, Y., Yamaguchi, M.
    • Journal Title

      Genes to Cells (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] -日本の遣伝学の潮流-Orbit/Claspタンパクファミリーによるショウジョウバエの染色体ダナミックス2007

    • Author(s)
      安藤至, 鈴木隆夫, 井上喜博
    • Total Pages
      340
    • Publisher
      NTS出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] -日本の遺伝学の潮流-Orbit/Claspタンパクファミリーによるショウジョウバエの染色体ダイナミックス2007

    • Author(s)
      安藤至, 鈴木隆夫, 井上喜博
    • Total Pages
      340
    • Publisher
      NTS出版
    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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