| Project/Area Number |
17580010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
AKIYAMA Takashi National Agricultural Research Organization, National Agricultural Research Center for Hokkaido Region, Research Team for Crop Cold Tolerance, Senior Researcher (10355456)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Rice / Polyamine / Arginine decarboxylase / Ornithine decarboxylase / Chilling stress / Gene expression / Promoter / Gene transfer / ポリアミン代謝酵素遺伝子 / 低温誘導性遺伝子 / 形質転換 / ストレス耐性の改善 / 形態学的変化の利用 / アルギニン脱炭素酵素 / cDNAクローニング / 発現解析 / プトレシン / スペルミジン / 品種改良 / 低温耐性 / ポリアミン代謝 / 遺伝子単離 / 形質転換体 / アグロバクテリウム法 |
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| Research Abstract |
Arginine decarboxylase (ADC; EC4.1.1.19) and ornithine decarboxylase (ODC; EC4.1.1.17) play pivotal roles in biosynthesis of putrescine, which is the precursor of the other two higher polyamines, spermidine and spermine. The polyamines have been implicated in many developmental processes and stress responses in higher plants. At the present time, however, only limited information is available for the regulation of polyamine biosynthesis in relation to ADC or ODC gene expression in plants in response to environmental stress. We have cloned ADC and ODC genes from rice, designated here as OsADC1and OsODC1, which encode putative ADC and ODC proteins. Only OsADC1 deduced protein is predicted to have an N-terminal putative signal peptide targeting to chloloroplasts, suggesting that the localization of OsADC1protein may differ from that of OsODC1 protein. Northern-blot analysis revealed that gene expression of OsADC1 was strongly induced by chilling stress, while OsODC1 gene expression increased in response to submergence stress. Interestingly, a concomitant increase in the levels of free putrescine coincided with the increase in levels of OsADC1 and OsODC1mRNA in response to chilling and submergence stress, respectively. To our knowledge, this is the first direct evidence to show that OsADC1 and OsODC1 genes are involved with the increase in putrescine levels that is induced in response to chilling stress and submergence stress in rice seedlings, respectively.
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