Acceleration of wound repair in gastric tract by lysophosphatidic acid, a wound-healing substance in vegetables
| Project/Area Number |
17580120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Fukuyama University |
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Principal Investigator |
SATOUCHI Kiyoshi Fukuyama University, Department of Applied Biological Science, Professor, 生命工学部, 教授 (50098125)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Tamotsu Fukuyama University, Department of Applied Biological Science, Associate professor, 生命工学部, 助教授 (90258301)
HIRANO Kaoru Fukuyama University, Department of Applied Biological Science, Assistant professor, 生命工学部, 助手 (90299305)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Biological molecule / biological activity / Foodstuff / Nutrition / Farm products |
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| Research Abstract |
Lysophosphatidic acid (LPA) is a growth-factor-like lipid that is thought to play a role in the process of wound healing. Here, we report production of LPA during digestion of vegetables. First, we examined amounts of LPA in homogenates of several kinds of vegetables and meats. We found that a homogenate of fresh cabbage leaf contained the largest amount of LPA (21 nmol/g wet weight). A large amount of phosphatidic acid (PA) was found (280 nmol/g) in cabbage leaf as well. In contrast to the fresh leaf, homogenates of boiled leaf contained neither PA nor LPA at significant levels, suggesting the involvement of activated phospholipase D in the production of PA and LPA during homogenization. Concentration of LPA (0.6 micro M) in the saliva obtained from masticated cabbage was biologically significant and much higher than normal saliva (0.06 micro M). Next, we examined LPA formation in the digestive tract and found that pancreatic phospholipase A2 hydrolyzes PA more effectively than phosphatidylcholine. Considering the presence of a large amount of PA in masticated cabbage leaf, additional LPA-formation by the action of digestive PLA2 can be occurred in lower digestive tract. Interestingly, a Chinese traditional medicine having analgesic and antiulcer actions has much LPA (60 nmol/g dry weight). But, another medicine having digestive tract-stimulating effect does not have LPA. We also found that cabbage lipid induce proliferation and migration of Swiss 3T3 fibloblasts. These responses are inhibitable by Ki 16425, an LPA antagonist. From these results, it was considered that ingestion of fresh vegetables, especially fresh cabbage leaves, is effective on acceleration of wound repair in gastric tract.
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Report
(3 results)
Research Products
(7 results)
