| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
Identification of the genes regulating neural progenitor neural stem cell functions is critical to understand the mechanisms of the adult neurogenesis and neurodegenerative disease. We compared the gene expression profile of proliferating neural stem cell culture with those of differentiated cells, and identified a novel gene named "Radmis (radial fiber associated mitotic spindle protein)". Northern blot analysis indicated radmis mRNA is expressed in the neural stem cells in vitro. In the developing and matured CNS, radmis protein was highly enriched in the mitotic neural progenitors. At E11 and E13 developing cerebral cortex, radmis expression was observed in the mitotic spindle, and radial fiber of the nestin-positive neuroepithelial cells, neural stem cells, located at the ventricular surface. At early postnatal CNS, radmis is expressed in positive neural progenitor cells in the SVZ of lateral ventricles, and 4th ventricle. In the developing cerebellum at P6, radmis is expressed in
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the mitotic spindle of the proliferating neuronal progenitor cells within the EGL layer, the superficial layer of cerebellum, but it was absent from the NeuN-positive post-mitotic neurons in both EGL arid IGL, the inner layer of the cerebellum. Radmis expression is drastically decreased as the CNS development proceeds, but a small number of cells in SVZ neurogenic region remained to express radmis protein. In the adult SVZ surrounding the lateral ventricles, radmis is expressed in the dividing neural stem cells (type B cells) that are positive for GFAP, and also in the proliferating neuronal progenitors (rapid amplifying cells, type C cells) that are positive for nestin. During the cell cycle, radmis protein did not expressed in the microtubules in interphase cells. At prometaphase, metaphase and anaphase, radmis colocalized with mitotic spindle poles and spindle microtubules proximal to the centrosomes, suggesting that radmis may control the spindle structure and function during the embryonic and adult neurogenesis. Less
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