Role of Interleukin in CD8^+T Cell contraction during Primary Infection
| Project/Area Number |
17590438
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Gunma University |
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Principal Investigator |
YAJIMA Toshiki Gunma University, Faculty of Medicine, Research Associate, 医学部, 助手 (20346852)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAI Yasunobu Kyushu University, Medical Institute of Bioregulation, Professor, 生体防御医学研究所, 教授 (90158402)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Infectious disease / Immunology / Bacteria |
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| Research Abstract |
During the course of acute infection with an intracellular pathogen, Ag-specific T cells proliferate in the expansion phase, and then most of the T cells die by apoptosis in the following contraction phase, but the few that survive become memory cells and persist for a long period of time. Although IL-15 is known to play an important role in long-term maintenance of memory CD8^+ T cells, the potential roles of IL-15 in CD8^+ T cell contraction are not known. Using an adoptive transfer system of OT-I cells expressing OVA_<257-264>/K^b-specific TCR into control, IL-15 knockout (KO) and IL-15 transgenic (Tg) mice followed by challenge with recombinant Listeria monocytogenes expressing OVA, we found that the survival of CD44+CD62L-CD127- effector OT-I cells during the contraction phase is critically dependent on IL-15. In correlation with the expression level of Bcl-2 in OT-I cells, the number of OT-I cells was markedly reduced in IL-15 KO mice but remained at a high level in IL-15 Tg mice during the contraction phase, compared with control mice. In vivo administration of rIL-15 during the contraction phase in IL-15 KO mice inhibited the contraction of effector OT-I cells accompanied by up-regulation of Bcl-2 expression. Furthermore, enforced expression of Bcl-2 protected the majority of effector OT-I cells from death in IL-15 KO mice after infection. These results suggest that IL-15 plays a critical role in protecting effector CD8^+ T cells from apoptosis during the contraction phase following a microbial infection via inducing antiapoptotic molecules.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Toll-Like receptors 2 and 4 are differentially involved in Fas dependent apoptosis in Peyer's patch and the liver at an early stage after bile duct ligation in mice.2006
Author(s)
Ogawa A, Tagawa T, Nishimura H, Yajima T, Abe T, Arai T, Taniguchi M, Takeda K, Akira S, Nimura Y, Yosikai Y.
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Journal Title
Related Report
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