• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of carcinogenesis in female reproductive organs induced by environmental estrogens

Research Project

Project/Area Number 17590511
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionMie University

Principal Investigator

MURATA Mariko  Mie University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (10171141)

Co-Investigator(Kenkyū-buntansha) KAWANISHI Shosuke  Suzuka University of Medical Science, Faculty of Health Science, Professor, 保健衛生学部, 教授 (10025637)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsEnvironmental estrogen / DNA damage / Reactive oxygen species / Activity of cell proliferation / Transition metal / Carcinogenrsis / Initiation / Promotion / 乳癌 / アルコール / salsolinol / イニシエーター / プロモーター / 活性酸素 / 環境化学物質 / プロシアニジンB2
Research Abstract

Chemical pollutant has some potential to alter estrogenic responses, and therefore its presence in the environment may be of concern for reproductive health. It may be involved with the increased incidence rates of breast cancer and endometrial cancer in most countries worldwide. Carcinogenesis is a multistage process, consisting of initiation, promotion, and progression phases. The tumor-initiation process starts DNA damage, leading to irreversible gene alteration. The tumor-promotion process requires sustained exposure to agents that stimulate the growth.
Salsolinol (Sal) is an endogenous metabolite of alcohol, generated by condensation reaction of dopamine with acetaldehyde, a major ethanol metabolite. Sal induced DNA damage including 8-oxo-7,8-dihydro-2'-deoxyguanosine formation in the presence of Cu(II) or Fe(III)EDTA. Catalase and metal-specific chelators inhibited SAL-induced DNA damage, suggesting the involvement of H2O2 and transition metals. SAL induced proliferation in estrogen-sensitive breast cancer MCF-7 cells, and the proliferation was inhibited by 4-hydroxytamoxifen, suggesting the involvement of estrogen receptor. In addition, SAL increased estrogen-independent mammary epithelial MCF-10A cells, and the proliferation was inhibited by an antioxidant N-acetylcysteine, suggesting that ROS may participate in proliferation of MCF-10A cells via EGFR activation. We demonstrated that SAL induces oxidative DNA damage and mammary cell proliferation, which may play important roles in tumor initiation and promotion of multistage breast cancer development in relation to alcohol drinking. Furthermore, we have revealed that plant polyphenol procyanidin B2(Free Radic. Biol. Med. 39, 1041-1049, 2005), air pollutant 3-nitrobenzanthrone (Free Radic. Biol. Med. 40, 1242-1249, 2006), and hair dye components ortho-phenylenediamine (Mutat. Res. 607, 184-191, 2006) have the DNA damaging abilities.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (41 results)

All 2007 2006 2005

All Journal Article (41 results)

  • [Journal Article] iNOS-dependent DNA damage in patients with malignant fibrous histiocytoma in relation to prognosis.2007

    • Author(s)
      Hoki, Y., Hiraku, Y., Ma, N., Murata M., Matsumine, A., Nagahama, M., Shintani, K., Uchida, A., Kawanishi, S.
    • Journal Title

      Cancer Sci. 98・2

      Pages: 163-168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] iNOS-dependent DNA damage in patients with malignant fibrous histiocytoma in relation to prognosis.2007

    • Author(s)
      Hoki, Y., Hiraku, Y., Ma, N., Murata, M., Matsumine, A., Nagahama, M., Shintani, K., Uchida, A., Kawanishi, S.
    • Journal Title

      Cancer Sci. 98

      Pages: 163-168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Tyrosine-dependent oxidative DNA damage induced by carcinogenic tetranitromethane.2006

    • Author(s)
      Murata M., Kurimoto, S., Kawanishi S.
    • Journal Title

      Chem Res Toxicol. 19・10

      Pages: 1379-1385

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Carcinogenic 3-nitrobenzanthrone induces oxidative damage isolated and cellular DNA.2006

    • Author(s)
      Murata M., Tezuka, T., Ohnishi, S., Takamura-Enya, Hisamatsu, Y., Kawanishi S.
    • Journal Title

      Free Radic Biol Med. 40・7

      Pages: 1242-1249

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Role of oxidative DNA damage in dietary carcinogenesis.2006

    • Author(s)
      Hiraku, Y., Murata, M., Kawanishi S.
    • Journal Title

      Genes Environ. 28・4

      Pages: 127-140

    • NAID

      110005717023

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxidative DNA damage induced by hair dye components ortho-phenylenediamines and the enhancement by superoxide dismutase.2006

    • Author(s)
      Murata M., Nishimura, T., Chen F., Kawanishi S.
    • Journal Title

      Mutat Res. 607・2

      Pages: 184-191

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 8-Nitroguanine formation in oral leukoplakia, a premalignant lesion.2006

    • Author(s)
      Ma, N., Tagawa, T., Hiraku, Y., Murata, M., Ding, X., Kawanishi S.
    • Journal Title

      Nitric Oxide. 14・2

      Pages: 137-143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] iNOS-dependent DNA damage via NF-kB expression in hamsters infected with Opisthorchis viverrini and its suppression by the antihelminthic drug praziquantel2006

    • Author(s)
      Pinlaor, S., Hiraku, Y., Yongvanit, P., Tada-Oikawa, S., Ma, N., Pinlaor, P., Sithithaworn, P., Sripa, B., Murata M., Oikawa, S., Kawanishi S.
    • Journal Title

      Int. J. Cancer 119・5

      Pages: 1067-1072

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mechanism of DNA damage induced by bromate differs from general types of oxidative stress.2006

    • Author(s)
      Kawanishi S., Murata, M.
    • Journal Title

      Toxicology 221・2-3

      Pages: 172-178

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tyrosine-dependent oxidative DNA damage induced by carcinogenic tetranitromethane.2006

    • Author(s)
      Murata, M., Kurimoto, S., Kawanishi, S.
    • Journal Title

      Chem Res Toxicol. 19

      Pages: 1379-1385

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Carcinogenic 3-nitrobenzanthrone induces oxidative damage isolated and cellular DNA.2006

    • Author(s)
      Murata, M., Tezuka, T., Ohnishi, S., Takamura-Enya, T., Hisamatsu, Y., Kawanishi, S.
    • Journal Title

      Free Radic Biol Med. 40

      Pages: 1242-1249

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Role of oxidative DNA damage in dietary carcinogenesis.2006

    • Author(s)
      Hiraku, Y., Murata, M., Kawanishi, S.
    • Journal Title

      Genes Environ. 28

      Pages: 127-140

    • NAID

      110005717023

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxidative DNA damage induced by hair dye components ortho-phenylenediamines and the enhancement by superoxide dismutase.2006

    • Author(s)
      Murata, M., Nishimura, T., Chen, F., Kawanishi, S.
    • Journal Title

      Mutat Res. 607

      Pages: 184-191

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 8-Nitroguanine formation in oral leukoplakia, a premalignant lesion.2006

    • Author(s)
      Ma, N., Tagawa, T., Hiraku, Y., Murata, M., Ding, X., _Kawanishi, S.
    • Journal Title

      Nitric Oxide. 14

      Pages: 137-143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] iNOS-dependent DNA damage via NF-kB expression in hamsters infected with Opisthorchis viverrini and its suppression by the antihelminthic drug praziquantel.2006

    • Author(s)
      Pinlaor, S., Hiraku, Y., Yongvanit, P., Tada-Oikawa, S., Ma, N., Pinlaor, P., Sithithaworn, P., Sripa, B., Murata, M., Oikawa, S., Kawanishi, S.
    • Journal Title

      Int. J. Cancer 119

      Pages: 1067-1072

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mechanism of DNA damage induced by bromate differs from general types of oxidative stress.2006

    • Author(s)
      Kawanishi, S., Murata, M.
    • Journal Title

      Toxicology 221

      Pages: 172-178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Carcinogenic 3-nitrobenzanthrone induces oxidative damage to isolated and cellular DNA.2006

    • Author(s)
      Murata M., Tezuka, T., Ohnishi, S., Takamura-Enya, T., Hisamatsu, Y., Kawanishi, S.
    • Journal Title

      Free Radic Biol Med. 40

      Pages: 1242-1249

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Tyrosine-dependent oxidative DNA damage induced by carcinogenic tetranitromethane.2006

    • Author(s)
      Murata, M., Kurimoto, S., Kawanishi.S.
    • Journal Title

      Chem Res Toxicol. 19

      Pages: 1379-1385

    • Related Report
      2006 Annual Research Report
  • [Journal Article] iNOS-dependent DNA damage via NF-κB expression in hamsters infected with Opisthorchis viverrini and its suppression by the antihelminthic drug praziquantel.2006

    • Author(s)
      Pinlaor, S., Hiraku, Y., Yongvanit, P., Tada-Oikawa, S., Ma, N., Pinlaor, P., Sithithaworn, P., Sripa, B., Murata, M., Oikawa, S., Kawanishi.S.
    • Journal Title

      Int. J. Cancer 119

      Pages: 1067-1072

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mechanism of DNA damage induced by bromate differs from general types of oxidative stress.2006

    • Author(s)
      Kawanishi S, Murata M.
    • Journal Title

      Toxicology (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Procyanidin B2 has anti-and pro-oxidant effects on metal-mediated DNA damage.2005

    • Author(s)
      Sakano, K., Mizutani, M., Murata M., Oikawa, S., Hiraku, Y., Kawanishi, S.
    • Journal Title

      Free Radic. Biol. Med 39・8

      Pages: 1041-1049

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitrative and oxidative DNA damage in intrahepatic holangiocarcinoma patients in relation to tumor invasion.2005

    • Author(s)
      Pinlaor, S., Sripa, B., Ma, N., Hiraku, Y., Yongvanit, P., Wongkham, S., Pairojkul, C., Bhudhisawasdi, V., Oikawa, S., Murata M., Semba, R., Kawanishi S.
    • Journal Title

      World J. Gastroenterol. 11・30

      Pages: 4644-4649

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxidative DNA damage in relation to neurotoxicity in the brain of mice exposed to arsenic at environmentally relevant levels.2005

    • Author(s)
      Piao, F., Ma, N., Hiraku, Y., Murata M., Oikawa, S., Cheng, F., Zhong, L., Yamauchi, T., Kawanishi, S., Yokoy ama, K.
    • Journal Title

      J. Occup. Health 47・5

      Pages: 445-449

    • NAID

      110004018352

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histone peptide AKRHRK enhances H_2O_2-induced DNA damage and alters its site specificity.2005

    • Author(s)
      Midorikawa, K., Murata, M., Kawanishi S.
    • Journal Title

      Biochem. Biophys. Res. Commun. 333・4

      Pages: 1073-1077

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evaluation for safety of antioxidant chemopreventive agents2005

    • Author(s)
      Kawanishi S., Oikawa, S., Murata M.
    • Journal Title

      Antioxid. Redox Signal. 7・11-12

      Pages: 1728-1739

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Accumulation of 8-nitroguanine in the liver of patients with chronic hepatitis C.2005

    • Author(s)
      Horiike, S., Kawanishi, S., Kaito, M., Ma, N., Tanaka, H., Fujita, N., Iwasa, M., Kobayashi, Y., Hiraku, Y., Oikawa, S., Murata, M., Wang, J., Semba, R., Watanabe, S., Adachi Y.
    • Journal Title

      J. Hepatol. 43・3

      Pages: 403-410

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitrative and oxidative DNA damage in oral lichen planus in relation to human oral carcinogenesis.2005

    • Author(s)
      Chaiyarit, P., Ma, N., Hiraku, Y., Pinlaor, S., Yongvanit, P., Jintakanon, D., Murata, M., Oikawa, S., Kawanishi, S.
    • Journal Title

      Cancer Sci. 96・9

      Pages: 553-559

    • NAID

      10017132692

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] DNA障害マーカー2005

    • Author(s)
      川西正祐, 村田真理子
    • Journal Title

      臨床検査 49・2

      Pages: 153-161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Procyanidin B2 has anti-and pro-oxidant effects on metal-mediated DNA damage.2005

    • Author(s)
      Sakano, K., Mizutani, M., Murata, M., Oikawa, S., Hiraku, Y., Kawanishi, S.
    • Journal Title

      Free Radic. Biol. Med. 39

      Pages: 1041-1049

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitrative and oxidative DNA damage in intrahepatic cholangiocarcinoma patients in relation to tumor invasion.2005

    • Author(s)
      Pinlaor, S., Sripa, B., Ma, N., Hiraku, Y., Yongvanit, P., Wongkham, S., Pairojkul, C., Bhudhisawasdi, V., Oikawa, S., Murata, M., Semba, R., Kawanishi, S.
    • Journal Title

      World J. Gastroenterol. 11

      Pages: 4644-4649

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oxidative DNA damage in relation to neurotoxicity in the brain of mice exposed to arsenic at environmentally relevant levels.2005

    • Author(s)
      Piao, F., Ma, N., Hiraku, Y., Murata, M., Oikawa, S., Cheng, F., Zhong, L., Yamauchi, T., Kawanishi, S., Yokoyama, K.
    • Journal Title

      J. Occup. Health 47

      Pages: 445-449

    • NAID

      110004018352

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Histone peptide AKRHRK enhances H2O2-induced DNA damage and alters its site specificity.2005

    • Author(s)
      Midorikawa, K., Murata, M., Kawanishi, S.
    • Journal Title

      Biochem. Biophys. Res. Commun. 333

      Pages: 1073-1077

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evaluation for safety of antioxidant chemopreventive agents.2005

    • Author(s)
      Kawanishi, S., Oikawa, S., Murata, M.
    • Journal Title

      Antioxid. Redox Signal. 7

      Pages: 1728-1739

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Accumulation of 8-nitroguanine in the liver of patients with chronic hepatitis C.2005

    • Author(s)
      Horiike, S., Kawanishi, S., Kaito, M., Ma, N., Tanaka, H., Fujita, N., Iwasa, M., Kobayashi, Y., Hiraku, Y., Oikawa, S., Murata, M., Wang, J., Semba, R., Watanabe, S., Adachi, Y.
    • Journal Title

      J. Hepatol. 43

      Pages: 403-410

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitrative and oxidative DNA damage in oral lichen planus in relation to human oral carcinogenesis.2005

    • Author(s)
      Chaiyarit, P., Ma, N., Hiraku, Y., Pinlaor, S., Yongvanit, P., Jintakanon, D., Murata, M., Oikawa, S., Kawanishi, S.
    • Journal Title

      Cancer Sci. 96

      Pages: 553-559

    • NAID

      10017132692

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Biomarker of DNA damage (Japanese)2005

    • Author(s)
      Murata, M., Kawanishi, S.
    • Journal Title

      Rioshi-kensa 49

      Pages: 153-161

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Evaluation for safety of antioxidant chemopreventive agents.2005

    • Author(s)
      Kawanishi S, Oikawa S, Murata M.
    • Journal Title

      Antioxid.Redox Signal. 7・11-12

      Pages: 1728-1739

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Oxidative DNA damage in relation to neurotoxicity in the brain of mice exposed to arsenic at environmentally relevant levels.2005

    • Author(s)
      Piao F, Ma N, Hiraku Y, Murata M, et al.
    • Journal Title

      J.Occup.Health. 47・5

      Pages: 445-449

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Procyanidin B2 has anti-and pro-oxidant effects on metal-mediated DNA damage.2005

    • Author(s)
      Sakano K, Mizutani M, Murata M, Oikawa S, Hiraku Y, Kawanishi S.
    • Journal Title

      Free Radio.Biol.Med. 39・8

      Pages: 1041-1049

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Histone peptide AKRHRK enhances H_2O_2-induced DNA damage and alters its site specificity.2005

    • Author(s)
      Midorikawa K, Murata M, Kawanishi S.
    • Journal Title

      Biochem.Biophys.Res.Commun. 333・4

      Pages: 1073-1077

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Accumulation of 8-nitroguanine in the liver of patients with chronic hepatitis C.2005

    • Author(s)
      Horiike S, Kawanishi S, et al.Murata M, Wang J, Semba R, Watanabe S, Adachi Y.
    • Journal Title

      J.Hepatol. 43・3

      Pages: 403-410

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi