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A role of mutated H-ferritin gene in famial gastric cancer -Analysis of mechanism using mutated H-ferritin transgenic mouse-

Research Project

Project/Area Number 17590663
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionSapporo Medical University

Principal Investigator

TAKAYAMA Tetsuji  Sapporo Medical University, School of Medicine, Associate professor, 医学部, 講師 (10284994)

Co-Investigator(Kenkyū-buntansha) KATO Junji  Sapporo Medical University, School of Medicine, Associate professor, 医学部, 助教授 (20244345)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsgastric cancer / ferriton
Research Abstract

We previously reported a family of gastric cancer, who had a germline mutation of A49T in H-ferritin gene (Am J Hum Genet. 69, 191-197, 2001). Therefore, in this study, we investigated the role of A49T mutation in H-ferritin gene in gastric carcinogenesis by in vitro transfection and analysis of transgenic mouse of the gene. When A49T mutated H-ferritin gene was transfected into COS-1 cells by lipofection method, promutagenic bases such as 8-hydroxy-deoxyguanosine (8-OHdG) were increased in the cells (particularly in nucleus), suggesting spontaneous DNA mutagenesis and carcinogenesis. Next, we constructed a gene targeting vector of A49T mutated H-ferritin gene, which included en-2 (splice acceptor), internal ribosome-entry site (IRES) of encephalomyeocarditis virus (EMCV), beta-galactosidase, beta-geo and SV 40 poly A signal. It was transfected into murine ES cells by electroporation, and 68 G418 resistant clones were selected. Of these, 15 clones with appropriate homologous recombination were obtained by PCR and Southern blotting. The correct sequences were confirmed in 9 clones. They were injected into murine blastcysts and they were transplanted into foster parent. Some chimerical mice were born, and heteromice (transgenic mice) were obtained by mating them with wild type mouse. Currently, we carefully observe transgenic mice and examine if they have a gastric cancer or cancers of the other organs or not.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (17 results)

All 2007 2006 2005

All Journal Article (17 results)

  • [Journal Article] Essential role of protein kinase C {zeta} in transducing a motility signal induced by superoxide and a chemotactic peptide, fMLP.2007

    • Author(s)
      Kuribayashi K, Takayama T, Kato J, Niitsu Y, et al.
    • Journal Title

      J Cell Biol 176

      Pages: 1049-1060

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Essential role of protein kinase C {zeta} in transducing a motility signal induced by2007

    • Author(s)
      Kuribayashi K, Takayama T, Kato J, Niitsu Y, et al.
    • Journal Title

      J Cell Biol 176

      Pages: 1049-1060

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Essential role of protein kinase C {zeta} in transducing a motility signal induced by superoxide and a chemotactic peptide, fMLP.2007

    • Author(s)
      Kuribayashi K, Takayama T, Niitsu, et al.
    • Journal Title

      Journal of cell Biology 176

      Pages: 1049-1060

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A phase I/II study of nedaplatin and 5-fluorouracil with concurrent radiotherapy in patients with esophageal cancer.2006

    • Author(s)
      Sato Y, Takayama T, Kato J, Niitsu Y, et al.
    • Journal Title

      Cancer Chemother Pharmacol 58

      Pages: 570-576

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Hepatitis C virus core protein promotes proliferation of human hepatoma cells through enhancement of transforming growth factor-{alpha} expression via activation of NF-{kappa}B.2006

    • Author(s)
      Sato Y, Kato J, Takayama T, Niitsu Y, et al.
    • Journal Title

      GUT 55

      Pages: 1801-1808

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ex vivo large scale generation of human platelets from cord blood CD34+ cells.2006

    • Author(s)
      Matsunaga T, Takayama T, Kato J, Niitsu Y, et al.
    • Journal Title

      Stemm Cells 24

      Pages: 2877-2887

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Amelioration of murine dextran sulfate sodium-induced colitis by ex vivo extracellular superoxide dismutase gene transfer.2006

    • Author(s)
      Oku T, Takayama T, Kato J, Niitsu Y, et al.
    • Journal Title

      Inflamm Bowel Dis 12

      Pages: 630-640

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A phase I/II study of nedaplatin and 5-fluorouracil with concurrent radiotherapy in patients with esophageal cancer.2006

    • Author(s)
      Sato Y, Takayama T, Kato J, Niitsu Y, et al.
    • Journal Title

      Cancer Chemother Pharmacol. 58

      Pages: 570-576

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Hepatitis C virus core protein promotes proliferation of human hepatoma cells through enhancement of transforming growth factor- {alpha} expression via activation of NF-{kappa}B.2006

    • Author(s)
      Sato Y, Kato J, Takayama T, Niitsu Y, et al.
    • Journal Title

      GUT 55

      Pages: 1801-1808

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ex vivo large scale generation of human platelets from cord blood CD34+ cells.2006

    • Author(s)
      Matsunaga.T, Takayama T, Kato J, Niitsu Y, et al.
    • Journal Title

      Stemm Cells 24

      Pages: 2877-2887

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Colorectal cancer : genetics of development and metastasis.2006

    • Author(s)
      Takayama T, Miyanishi K, Hayashi T, Sato Y, Niitsu Y
    • Journal Title

      Journal Of Gastroenterology 40

      Pages: 185-192

    • NAID

      10017477036

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Amelioration of murine dextran sulfate sodium-induced colitis by exvivo extracellular superoxide dismutase gene transfer.2006

    • Author(s)
      Oku T, Takayama T, Niitsu Y, et al.
    • Journal Title

      Inflammatory Bowel Disease 12

      Pages: 630-640

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Human mesenchymal stem cells xenografted directly to rat liver differentiated into human hepatocytes without fusion.2005

    • Author(s)
      Sato Y, Kato J, Takayama T, Niitsu Y, et al.
    • Journal Title

      Blood 106

      Pages: 756-763

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Augmentation of Antitumor Effects of p53 Gene Therapy by Combination with HDAC Inhibitor.2005

    • Author(s)
      Takimoto R, Kato J, Takayama T, Niitsu Y, et al.
    • Journal Title

      Cancer Biol Ther 4

      Pages: 421-428

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Augmentation of Antitumor Effects of p53 Gene Therapy by Combination with HDAC Inhibitor.2005

    • Author(s)
      Takimoto R, Kato J, Takayama T, Niitsu Y, et al.
    • Journal Title

      Cancer Biol Ther. 4

      Pages: 421-428

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Aberrant crypt foci : detection, gene abnormalities, and clinical usefulness.2005

    • Author(s)
      Takayama T, et al.
    • Journal Title

      Clin Gastroenterol Hepatol. 3

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Augmentation of antitumor effects of p53 gene therapy by combination with HDAC inhibitor.2005

    • Author(s)
      Takimoto R, Kato J, Takayama T et al.
    • Journal Title

      Cancer Biol Ther 4

      Pages: 421-428

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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