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Study of gene expression by DNA chip in peripheral blood and salivary epithelial cells from patients with Sjogren's syndrome

Research Project

Project/Area Number 17591059
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionHamamatsu University School of Medicine (2006-2007)
Kanazawa Medical University (2005)

Principal Investigator

OGAWA Noriyoshi  Hamamatsu University School of Medicine, Unirersit Hospital, Assistant Professor (80308618)

Co-Investigator(Kenkyū-buntansha) UMEHARA Hisanori  Kanazawa Medical University, Dept of Hematol&Immunol, Professor (70247881)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,730,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsSiogren's syndrome / peripheral blood / salivary island / gene expression / DNA microarray / 悪性リンパ腫 / 遺伝子 / DNA array / ribosomal protein / 唾液腺上皮細胞 / インターフェロン / IL-1β
Research Abstract

Purpose: To identify gene expression pattern of peripheral blood and salivary epithelial cells from patients with Sjogren's syndrome (SS), DNA microarray analysis was performed.
Methods: 14 primary SS (14 females, 59.3± 13.8 year-old), 5 secondary SS patients (5 females, 60.6 ±5.0 year-old) were subjected for the study. All patients fulfilled the 1999 revised Japanese criteria for SS. Total RNA was isolated with PAX gene Blood RNA system immediately after blood collection. DNA microarray analysis was done using a low density cDNA microarray system with 778 genes (Japan Genome Solutions). Reference RNA was from ten healthy donors (5 males and 5 females, mean age 39.3 year-old). Ten patients with rheumatoid arthritis (RA) were served as disease control. The relationship of gene signature with various clinical parameters, such as disease duration, symptoms and signs, complications, immunological findings, salivary and lacrimal functions was analyzed. Cultured salivary epithelial cells from … More three SS patients were subjected for DNA microarray analysis, and compared with normal salivary epithclial cells. Results: I)IFN-inducible genes were upregulated in primary and secondary SS. 2) The gene expression pattern of primary and secondary SS was similar, but was different from RA. There were several genes that could distinguish primary SS from secondary SS, some of which were IFN-inducible genes. IFN gene signature was more prominent in primary SS compared to secondary SS. 3) The expression level of interferon alpha-inducible protein 27 (IFI27) showed significantly positive correlation with serum IgG level in SS (r=0.600, p<0.01). 4) Many ribosomal protein genes were upregulated in SS patients with maltoma. When the gene-expression was compared before and after chemotherapy, the expression level of ribosomal protein S27 and S29 was decreased in an SS patient with maltoma. 5) There was not significant gene expression pattern in SS salivary epithelial cells compared with normal salivary epithelial cells. Conclusions: SS peripheral blood has unique gene signature compared to RA. IFN-inducible gene signature may be related to greater immunological abnormality. By using such molecules as ribosomal protein S27 and S29, molecular prediction of lymphoma development might be possible in SS. Less

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005

All Journal Article (7 results) (of which Peer Reviewed: 1 results) Presentation (11 results)

  • [Journal Article] Peripheral blood from Sjogren's syndrome patients with malignant lymp homa has unique gene expression signatures2007

    • Author(s)
      Noriyoshi Ogawa, et. al.
    • Journal Title

      Ann Rheum Dis 66

      Pages: 312-312

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Peripheral blood from Sjogren's syndrome patients with malignant lymphoma has unique gene expression signatures2007

    • Author(s)
      Noriyoshi, Ogawa, et. al.
    • Journal Title

      Ann Rheum Dis 66

      Pages: 312-312

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Peripheral blood from Sjogren's syndrome patients with malignant lymphoma has unique gene expression signatures2007

    • Author(s)
      Noriyoshi Ogawa, et. al.
    • Journal Title

      Ann Rheum Dis 66

      Pages: 312-312

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA Microarray Analysis Of Gene Expression Of Peripheral Blood From Patient s With Sjogren's Syndrome2006

    • Author(s)
      Noriyoshi Ogawa, et. al.
    • Journal Title

      Arthritis & Rheumatism 54

      Pages: 707-708

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] DNA microarray analysis of gene expression of peripheral blood from patients with Sjogren's syndrome2006

    • Author(s)
      Noriyoshi, Ogawa, et. al.
    • Journal Title

      Arthritis & Rheumatism 54

      Pages: 707-708

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] DNA Microarray Analysis Of Expression Of Peripheral Blood From Patients With Sjogren's Syndrome2006

    • Author(s)
      Noriyoshi Ogawa et al.
    • Journal Title

      Arthritis & Rheumatism 54・9

      Pages: 707-708

    • Related Report
      2006 Annual Research Report
  • [Journal Article] DNA microarray analysis of gene signature of peripheral blood from patients with Sjogren's syndrome2005

    • Author(s)
      Ogawa N, et al.
    • Journal Title

      日本免疫学会学術集会記録 35

      Pages: 39-39

    • Related Report
      2005 Annual Research Report
  • [Presentation] Peripheral blood from Sjogren's syndrome patients with malignant lymphoma has unique gene expression signatures2007

    • Author(s)
      Noriyoshi Ogawa, et. al.
    • Organizer
      2007年ヨーロッパリウマチ学会
    • Place of Presentation
      Barcelona, Spain
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Peripheral blood from Sjogren's syndrome patients with malignant lymphoma has unique gene expression pattern2007

    • Author(s)
      小川法良、他
    • Organizer
      第37回日本免疫学会総会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Peripheral blood from Sjogren's syndrome patients with malignant lymphoma has unique gene expression signatures2007

    • Author(s)
      Noriyoshi, Ogawa, et. al.
    • Organizer
      European League Association of Rheumatology Meeting
    • Place of Presentation
      Barcelona, Spain
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Peripheral blood from Sjogren's syndrome patients with malignant lymphoma has unique gene expression pattern2007

    • Author(s)
      Noriyoshi, Ogawa, et. al.
    • Organizer
      37th Japan Society for Immunology Meeting
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Peripheral blood from Sjogre's syndrome patients with mahgnant lymp homa has unique gene expression pattern2007

    • Author(s)
      Noriyoshi Ogawa, et. al.
    • Organizer
      第37回日本免疫学会総会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] DNA Microarray Analysis Of Gene Expression Of Peripheral Blood From Patients With Sjogren's Syndrome2006

    • Author(s)
      Noriyoshi Ogawa, et. al.
    • Organizer
      2006年アメリカリウマチ学会
    • Place of Presentation
      Washington DC, USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA microarray analysis of gene expression of peripheral blood from patients with Sjogren's syndrome2006

    • Author(s)
      Noriyoshi, Ogawa, et. al.
    • Organizer
      American College of Rheumatology Meeting
    • Place of Presentation
      Washington DC, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] シェーグレン症候群末梢血および唾液腺上皮細胞における遺伝子発現のDNA array解析2005

    • Author(s)
      小川法良、他
    • Organizer
      第49回日本リウマチ学会総会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA microarray analysis of gene signature of peripheral blood from patients wit hSjogren's syndrome2005

    • Author(s)
      小川法良、他
    • Organizer
      第35回日本免疫学会総会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA microarray analysis of gene expression of peripheral blood and salivary epithelial cells from patients with Sjogren's syndrome2005

    • Author(s)
      Noriyoshi, Ogawa, et. al.
    • Organizer
      49th Japan Rheumatism Association Meeting
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] DNA microarray analysis of gene signature of peripheral blood from patients with Sjogren's syndrome2005

    • Author(s)
      Noriyoshi, Ogawa, et. al.
    • Organizer
      35th Japan Society for Immunology Meeting
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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