Basic study for the improvement of melanoma radiotherapy by suppression of TRP-2 gene expression
| Project/Area Number |
17591301
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Kobe Gakuin University |
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Principal Investigator |
KONDOH Hirofumi Kobe Gakuin University, The Faculty of Pharmaceutical Sciences, Researcher, 薬学部, 研究員 (20399132)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUMORI Yoshinobu Kobe Gakuin University, The Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (60102927)
HIRATUKA Junichi Kawasaki Medical School, Department of Radiation Oncology, Associate Professor, 放射線医学(治療), 助教授 (30192298)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Radiation / X-ray / Particle-beam / Gene / Cancer / Control of gene expression / Resistance / Therapy |
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| Research Abstract |
TRP-2 gene is specifically expressed in melanoma cells, and is thought to related to the radio-resistance of melanoma. The aim of this project is to establish the method of improvement of radiotherapy for melanoma by suppression of TRP-2 gene expression, cultured melanoma cell level experiments were performed as the first step.
The results obtained through this research project are summarized as follows :
1. TRP-2 sh-RNA expression plasmids were transfected to tyrosinase-ecpression deficient amelanotic melanoma cell to construct TRP-2 expression deficient melanoma cell lines.
2. Evaluation systems for the killing effect of X-ray, UV, and cisplatin were established to the effect of TRP-2 expression in melanoma cells.
3. Evaluation system for measuring the content of melanin monomer DHICA, which is the product of TRP-2, was established.
4. Inversely, to evaluate the effect of TRP-2 expression in cells, non-melanoma cultured cell, HeLa cells were transfected with TRP-2 expression plasmid. Using this cell line we can clarify whether the radiation-resistance of melanoma is related to the expression and production of TRP-2 protein or melanogenesis related to TRP-2. We evaluated the properties of these cell lines concerning melanogenesis, cell biology, and molecular biology.
Comparing the these properties of established cell lines, we could not obtained clear results that TRP-2 gene suppression improve the killing effect of X-ray radiation, UV, and cisplatin. Currently, we improve the evaluation systems and methods, and continue to the evaluation of the effects.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Intracelluar composition of gatty acid affects the processing and function of tyrosinase through the ubiquitin-proteasome pathway.2006
Author(s)
Ando H, Wen ZM, Kim HY, Valencia JC, Costin GE, Watabe H, Yasumot K, Niki Y, Kondoh H, Ichihahi M, Hearing VJ.
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Journal Title
Biochem J. 394
Pages: 43-50
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Impact of intra-arterial administration of boron compounds on dose-volume histograms in boron neutron capture therapy for recurrent head-and-neck tumors.2006
Author(s)
Suzuki M, Sakurai Y, Nagata K, Kinashi Y, Masunaga S, Ono K, Maruhashi A, Kato I, Fuwa N, Hiratsuka J, Imahori Y.
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Journal Title
Int J Radiat Oncol Biol Phys. 66
Pages: 1523-1527
Description
「研究成果報告書概要(欧文)」より
Related Report
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