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Development of Cell Transplantation System Using A Closed Culture for Heart Diseases

Research Project

Project/Area Number 17591486
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionSaitama Medical University

Principal Investigator

KYO Shunei  Saitama Medical University, Faculty of Medicine, Professor, 医学部, 教授 (30153232)

Co-Investigator(Kenkyū-buntansha) GOJO Satoshi  Saitama Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90316745)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsMesenchymal Stem Cell / Cell Culture / Cell Transplantation / Ischemic Heart Disease
Research Abstract

In regenerative medicine using cell processing, a cell culture system is one of the highest obstacles. Most clinical trials in cultivated cell therapy needed huge cell processing center, which ensure the quality of manufacturing products. Our study examined the closed cell culture bottle, and resulted in good feasibility and safety in the first year. The differentiation capability and proliferation ability in this culture system were the same as those cultivated in open culture systems.
Initially, we planned the clinical trial for peripheral arterial disease using the close cell culture system in the second year. However, the regulation for the cell processing would be stricter in the start of the second year, and we could not perform the clinical trial. We have focused in the safety of long-term cultivated cells in vivo environment, which is one to be examined in vitro environment in this study. Especially, carcinogenesis following the long-term culture is recognized in vitro. For cell therapy, the risk of carcinogenesis is carefully considered. In beagle dogs, autologous bone marrow-derived mesenchymal cell transplantation to the heart was performed. The cells had been cultivated for 5 to 10 passages before grafting. After 1 to 6 months, the histopathological analyses of the hearts were carried out. Neither carcinoma nor dysplasia was recognized. Neither heteroplasia nor inflammation was recognized.
Mid-term cell culture could not induce carcinogenic changes. Our results suggest that cells passaged under 10 times would be not significant carcinogenic. At present, a company produced a robotic culture system for completely closed cell culture system in a trial version. Robotics strategy would reduce the cost and labor in this process.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005

All Journal Article (18 results)

  • [Journal Article] Cardiac Resurrection following Bone Marrow-derived Mononuclear Cell Transplantation during Left Ventricular Assist Device Support2007

    • Author(s)
      Satoshi Gojo
    • Journal Title

      Ann. Thorac. Surg 83(2)

      Pages: 661-662

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells.2007

    • Author(s)
      Y Yamada
    • Journal Title

      Exp.Cell.Res. 313(4)

      Pages: 698-706

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 再生医療と心疾患2007

    • Author(s)
      五條理志
    • Journal Title

      ホルモンと臨床 5・55

      Pages: 451-457

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 細胞移植による心臓疾患への再生療法 -骨髄間葉系細胞移植・骨格筋芽細胞移植・骨髄単核球細胞-2007

    • Author(s)
      五條理志
    • Journal Title

      Organ Biology 14・1

      Pages: 41-51

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Cardiac Resurrection following Bone Marrow-derived Mononuclear Cell Transplantation during Left Ventricular Assist Device Support2007

    • Author(s)
      Satoshi Gojo, Shunei Kyo, Shigeyuki Nishimura, Nobuyuki Komiyama, Nobutaka Kawai, Masami Bessho, Hiroshige Sato, Toshihisa Asakura, Motonobu Nishimura, Kenji Ikebuchi
    • Journal Title

      Ann. Thorac. Surg 83

      Pages: 661-2

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells2007

    • Author(s)
      Yoji Yamada, Kazuhiro Sakurada, Yukiji Takeda, Satoshi Gojo, Akihiro Umezawa
    • Journal Title

      Exp.Cell.Res. 313(4)

      Pages: 698-706

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 再生医療と心疾患2007

    • Author(s)
      五條理志
    • Journal Title

      ホルモンと臨床 55(5)

      Pages: 451-457

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 細胞移植による心臓疾患への再生療法 -骨髄間葉系細胞移植・骨格筋芽細胞移植・骨髄単核球細胞-2007

    • Author(s)
      五條理志
    • Journal Title

      Organ Biology 14(1)

      Pages: 41-51

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Increased mobilization of c-kit(+) Sca-1(+) Lin(-) (KSL) cells and colony-forming units in spleen (CFU-S) following de novo formation of a stem cell niche depends on dynamic, but not stable, membranous ossification.2006

    • Author(s)
      Nagayoshi K
    • Journal Title

      J Cell Physiol. 208

      Pages: 188-194

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Transthoracic direct current shock facilitates intramyocardial transfection of naked plasmid DNA infused via coronary vessels in canines.2006

    • Author(s)
      Iida Y
    • Journal Title

      Gene Ther. 13

      Pages: 909-916

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart.2006

    • Author(s)
      Tsunoda S
    • Journal Title

      Biochem Biophys Res Commun. 336(1)

      Pages: 118-127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 骨髄細胞移植による心臓再生-左室補助人工心臓離脱を目指して-2006

    • Author(s)
      五條理志
    • Journal Title

      循環制御 27(3)

      Pages: 203-208

    • NAID

      10020334883

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 心臓再生医療2006

    • Author(s)
      五條理志
    • Journal Title

      Clinical Engineering 17(8)

      Pages: 875-880

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] 心不全の再生治療2006

    • Author(s)
      五條理志
    • Journal Title

      呼吸と循環 54(10)

      Pages: 1123-1128

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Increased mobilization of c-kit(+) Sca-1(+) Lin(-) (KSL) cells and colony-forming units in spleen (CFU-S) following de novo formation of a stem cell niche depends on dynamic, but not stable, membranous ossification2006

    • Author(s)
      Nagayoshi K, Ohkawa H, Yorozu K, Higuchi M, Higashi S, Kubota N, Fukui H, Imai N, Gojo S, Hata JI, Kobayashi Y, Umezawa A
    • Journal Title

      J Cell Physiol. 208

      Pages: 188-194

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Transthoracic direct current shock facilitates intramyocardial transfection of naked plasmid DNA infused via coronary vessels in canines2006

    • Author(s)
      Iida Y, Oda Y, Nakamori S, Tsunoda S, Kishida T, Gojo S, Shin-Ya M, Asada H, Imanishi J, Yoshikawa T, Matsubara H, Mazda 0
    • Journal Title

      Gene Ther. 13

      Pages: 906-916

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 骨髄細胞移植による心臓再生-左室補助人工心臓離脱を目指して2006

    • Author(s)
      五條理志
    • Journal Title

      循環制御 27(3)

      Pages: 203-208

    • NAID

      10020334883

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Sonoporation using microbubble BR14 promotes pDNA/siRNA transduction to murine heart2005

    • Author(s)
      Tsunoda S, Mazda 0, Oda Y, Iida Y, Akabame S, Kishida T, Shin-Ya M, Asada H, Gojo S, Imanishi J, Matsubara H, Yoshikawa T
    • Journal Title

      Biochem Biophys Res Commun. 336(1)

      Pages: 118-127

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2005-04-01   Modified: 2016-04-21  

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