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Intrinsic stem cell promote neuronal regeneration by oligomerization of A β 1-42monomer

Research Project

Project/Area Number 17591513
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKobe University

Principal Investigator

KONDOH Takeshi  Kobe University, School of Medicine, Associate Professor (50273769)

Co-Investigator(Kenkyū-buntansha) MATSUMOTOQ Akira  Foundation for Biomedical Research and Innovation, 脳疾患病態解析部, Director (80181759)
KOHMURA Eiji  Kobe University, Graduate School of Medicire, Professor (30225388)
三宅 茂  神戸大学, 医学部附属病院, 助手 (30362784)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,680,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsBone marrow stromal cells / organotopic hipocampal slice / 海馬切片培養 / 幹細胞 / ベータアミロイド / 骨髄間質細胞
Research Abstract

Bone marrow stromal cells taken from EGFP transgenic mice were sorted by magnetic beads with surface markers of Sca-1 and Thy-1. The cells were then co-cultured on organotopic hippocampal slice or with neuronal cell feeder in dish. On hippocampus, both Sca-1 and Thy-1 positive cells showed 4- 8 folds higher potential to show neuron-like morphology than negative cells. In dish, negative cells fewly survived but each positive cells survived and showed neuron-like differentiation. In both culture condition, retinoic acid supplement accelerate differentiation. Differentiated Sca-1 and Thy-1 positive cells were immunohistochemically GFAP- and NeuN-negative but nestin-, neurofilament and NSE-positive. Neuron-like differentiation of bone marrow cells can be enhanced by selection using cell surface proteins.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (8 results)

All 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] Effect of VEGF Receptor Antagonist (VGA1155) on Brain Edema in the Rat Cold Injury Model.2007

    • Author(s)
      Koyama J, Miyake S, Sasayama T, Kondoh T, Kohmura e
    • Journal Title

      Kobe J Med Sci. 53

      Pages: 199-207

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of VEGF Receptor Antagonist (VGA1155) on Brain Edema in the Rat Cold Injury2007

    • Author(s)
      Koyama, J, Miyake, S, Sasayama, T, Kondoh, T, Kohmura, E
    • Journal Title

      Kobe J Med Sci 53

      Pages: 199-207

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of VEGF Receptor Antagonist(VGA1155)on Brain Edema in the Rat Cold Injury Model.2007

    • Author(s)
      Koyama J, Miyake S, Sasayama T, Kondoh T, Kohmura E
    • Journal Title

      Kobe J Med Sci. 53

      Pages: 199-207

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sca-1 and Thy-1 accelerate neuron-like differentiation in bone marrow stromal cells2006

    • Author(s)
      Mizobe T, Kidoguchi K, Tamaki M, Sasayama T, Kondoh T, Kohniura E
    • Journal Title

      Kohe J Med Sci. 52(5)

      Pages: 119-125

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Carotid Artery Occlusion and Vasculature Change in C57Black/J Mice Detected by Synchrotron Radiation Microangiography2006

    • Author(s)
      Tamaki M, Kidoguchi K, Mizobe T, Koyama J, Kondoh T, Kohmura E, Sakurai T, Yokono K, Umetani K
    • Journal Title

      Kobe J Med Sci. 52(2)

      Pages: 111-118

    • Related Report
      2006 Annual Research Report
  • [Journal Article] In Vivo X-ray Angiography in the Mouse Brain using Synchrotron Radiation2006

    • Author(s)
      Kidoguchi K, Tamaki M, Mizobe T, Koyama J, Kondoh T, Kohmura E, Sakurai T, Yokono K, Umetani K
    • Journal Title

      Stroke 37(7)

      Pages: 1856-1861

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Impaired response of perforating arteries to hypercapnia in chronic hyperglycemia2006

    • Author(s)
      Oizumi XS, Akisaki T, Kouta Y, Song XZ, Takata T, Kondoh T, Umetani K, Hirano M, Yatnasaki K, Kohmura E, Yokono K, Sakurai T.
    • Journal Title

      Kobe J Med Sci. 52(2)

      Pages: 27-35

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Quantification of distension in rat cerebral perforating arteries2006

    • Author(s)
      Morishita A, Kondoh T, Sakurai T, Ikeda M, Bhattacharjee AK, Nakajima S, Kohmura E, Yokono K, Umetani K.
    • Journal Title

      Neuroreport 17(14)

      Pages: 1549-1553

    • Related Report
      2006 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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