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Basic study of gene therapy for severe pain by using virus vector

Research Project

Project/Area Number 17591612
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionUniversity of Tsukuba

Principal Investigator

INOMATA Shinichi  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Associate Professor (10282352)

Co-Investigator(Kenkyū-buntansha) MIYOSHI Hiroyuki  RIKEN Bio Resource Center, Subteam for Manipulation of Cell Fate, Team leader (70219830)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥120,000)
Fiscal Year 2007: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordssevere pain / enkephalin / virus vector / gene therapy / ウイルスベクター / ペインクリニック / 疼痛
Research Abstract

Total RNA of a human adrenal gland was obtained from patients, received surgical operation, with their informed consents. The cDNA was synthesized by reverse transcriptase using a primer. The pre-pro-enkephalin (PPE) gene was then amplified by polymerase chain reaction with specific upstream and downstream oligoneucleotides. The polymerase chain reaction product contained the full-length PPE coding cDNA. The amplified fragment was cloned into a vector (pDON-AI-2-Neo or pMEI-5 Neo [Not I-BamHI]). The plasmid DNA was purified and digested with restriction enzymes and electrophoresed on agarose gel. The higher efficacy of infection was observed in pDON-AI-2-Neo.

Report

(4 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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