| Project/Area Number |
17591765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
TSUJI Yoshiyuki Hyogo College of Medicine, Faculty of Medicine, Part-time Assistant Professor (60148658)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMURA Haruki Hyogo College of Medicine, Faculty of Medicine, Professor (60111043)
HASHIMOTO Tomoko (TAMAOKI, Tomoko) Hyogo College of Medicine, Faculty of Medicine, Professor (10172868)
長田 久美子 兵庫医科大学, 医学部, 助教授 (90068502)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,670,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Mycoplasma / Ureaplasma / Abortion / Infertility / immunodeficiency / IL-8 / TLR / IL-18 |
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| Research Abstract |
Ureaplasma urealyticum is a member of the family Mycoplasmataceae and often observed in the vagina of pregnant women. In this study, we examined if ureaplasmal infection is associated with preterm delivery and whether ureaplasmal lipoprotein (LP) has the capacity to stimulate monocytes to produce pro-inflammatory cytokines relevant to preterm delivery in vitro. We selected 100 cases of normal delivery and 45 cases of preterm delivery at random. We generated a monoclonal antibody (mAb) against U.urealyticum urease that selectively and positively stained it in vaginal secretions of infected women but not of uninfected women. The preterm delivery group showed significantly higher incidence for vaginal infection with this bacteria than the normal delivery group. Since LP of Mycoplasma has a potent biological activity, we extracted ureaplasmal LP. THP-1 cells, human monocytic cells, produced interleukin (IL)-8, a potent pro-inflammatory cytokine associated with preterm delivery, and showed apoptotic cell death in response to the LP. These results suggested that U. urealyticum infection might play a causative role in preterm delivery via LP-induced of IL-8 production and apoptosis.
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