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Development of a new high-throughput TCR-antigen pair identification method toward cancer immune therapy

Research Project

Project/Area Number 17F17389
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Genome biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

城口 克之  国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (00454059)

Co-Investigator(Kenkyū-buntansha) JIN JIANSHI  国立研究開発法人理化学研究所, 生命機能科学研究センター, 外国人特別研究員
Project Period (FY) 2017-10-13 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2019: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 2018: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2017: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsイメージング / ピッキング / 細胞相互作用 / T細胞 / TCR / T cell / sequencing / cell-cell interaction / microscopy
Outline of Annual Research Achievements

We have been building up a microscopic platform for scanning the real-time cell behaviors, such as cell movement and T cell-target cell interactions. For high-throughput scanning of these behaviors, we have checked that scanning rate of the current system, and found that the speed of stage movement may be enough for the observation of T cell activities. However, in order to link the microscope system to following steps, we found that real-time calculation based on obtained cell images was required. Then, we investigated specifications of a software based on our actual measurements for T cell-target cell interactions. This helps one to combine imaging and following genomic analyses using our cell picking system.

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (4 results)

All 2019 2018 2017

All Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] Probing Taxa-Specific Bacterial Coordinational Distribution in Intestinal Microbiota by Super-accurate Method BarBIQ2019

    • Author(s)
      J. Jin, R. Yamamoto, T. Takeuchi, E. Miyauchi, H. Ohno, K. Shiroguchi
    • Organizer
      EMBL Symposia
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An applicational study of a novel developed method BarBIQ: analysis of microbiota in different locations of a murine cecum & colon2018

    • Author(s)
      Janshi Jin, Reiko Yamamoto, Tadashi Takeuchi, Eiji Miyauchi, Hiroshi Ohno, Katsuyuki Shiroguchi
    • Organizer
      The 47th Annual Meeting of The Japanese society of Immunology
    • Related Report
      2018 Annual Research Report
  • [Presentation] A novel high-throughput method for quantification of microbiota distribution at the single cell level2017

    • Author(s)
      Jianshi Jin, Tadashi Takeuchi, Eiji Miyauchi, Hiroshi Ohno, Katsuyuki Shiroguchi
    • Organizer
      CSH Asia on “Tumor Immunology & Immunotherapy”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel high-throughput method for bacteria counting and de-novo 16S rRNA sequence identification with single-base resolution2017

    • Author(s)
      Jianshi Jin1, Kaori Fukuhara, Tadashi Takeuchi, Eiji Miyauchi, Hiroshi Ohno, Katsuyuki Shiroguchi
    • Organizer
      The 46th Annual Meeting of The Japanese society of Immunology
    • Related Report
      2017 Annual Research Report

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Published: 2017-10-17   Modified: 2024-03-26  

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