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Study of Rapid Detection Technology for Oil Quality and Toxicological Evaluation of Frying Oil

Research Project

Project/Area Number 17F17751
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Food science
Research InstitutionHokkaido University (2018-2019)
Akita Prefectural University (2017)

Principal Investigator

惠 淑萍 (2018-2019)  北海道大学, 保健科学研究院, 教授 (90337030)

張 函 (2017)  秋田県立大学, 生物資源科学部, 准教授 (10315608)

Co-Investigator(Kenkyū-buntansha) MA JIN-KUI  北海道大学, 保健学研究科, 外国人特別研究員
Project Period (FY) 2017-11-10 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2019: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2018: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2017: ¥500,000 (Direct Cost: ¥500,000)
KeywordsHydroperoxide, / cell toxicity, / fatty acid / triacylglycerol / TGOOH / hydroperoxides / Frying oil / quality evaluation / e-nose / polar compounds / toxicity / in vitro
Outline of Annual Research Achievements

It was established that an effective and rapid detection method based on the odor fingerprint of frying oil for a real-time detection and monitoring of frying oil quality. The potential toxicities of frying oils' polar substances and oxidative products (hydroperoxides) to cells in vivo were investigated. The major primary oxidative products of the linoleic acid, 9 and 13-Hydroxyoctadecadienoic acid (9 and 13-HPODE), were synthesized using soybean lipoxygenase I-B. The 9 and 13-HPODE were also subjected to HepG 2 cells to evaluate its possible adverse effects (cell viability, changes in the enzyme expression, lipidomic analysis) . The research results of 2019 are summarized as follows:
1.The synthesized hydroperoxides have been identified by the Mass spectroscopy and NMR. Results showed that the purity of the synthesized 13-HPODE was around 95% and the yield rate was 70% above which implies the method is successful and was feasible for preparing 9 and 13-HPODE in a regular laboratory.
2.In the cells’ experiments, there were two parallel experiments carried out: one is for the PAHs and another is for the HPODEs using target cell line- HepG 2 cells. Results showed that the mRNA expression of GSTO1, SOD2, and HO-1 have increased when the cells were treated with high HPODEs concentration (100 uM), while the GPX mRNA expression was inhibited after the treatment. Results also indicated that the expression level of CYP1A1 mRNA has been significantly increased after the cells treated with high concentration of HPODEs.

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (4 results)

All 2019 2018

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] Reversal of homocysteine-induced neurotoxicity in rat hippocampal neurons by astaxanthin: evidences for mitochondrial dysfunction and signaling crosstalk2018

    • Author(s)
      Xian-jun Wang, Wang Chen, Xiao-ting Fu, Jin-kui Ma, Mei-hong Wang, Ya-jun Hou, Da-chen Tian, Xiao-yan Fu, Cun-dong Fan
    • Journal Title

      cell death discovery

      Volume: 4 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41420-018-0114-x

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reversal of homocysteine-induced neurotoxicity in rat hippocampal neurons by astaxanthin: evidences for mitochondrial dysfunction and signaling crosstalk2018

    • Author(s)
      Xian-jun Wang; Wang Chen, Xiao-ting Fu, Jin-kui Ma, Mei-hong Wang, Ya-jun Hou, Da-chen Tian, Xiao-yan Fu, Cun-dong Fan
    • Journal Title

      Molecular Medicine Reports

      Volume: 419 Pages: 4753-4760

    • DOI

      10.3892/mmr.2019.10162

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Fried oil-induced changes in xenobiotic metabolizing enzymes in human HepG2 cells2019

    • Author(s)
      Jin-Kui Ma,Wageh Sobhy Darwish, Min-Cheng Gao, Hui Tan, Hitoshi Chiba, Shu-Ping Hui
    • Organizer
      第99回北海道医学大会 臨床検査医学分科会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Quality evaluation of rapeseed oils based on the changes of volatile flavor and unsaturated fatty acids during frying2018

    • Author(s)
      Jin-Kui Ma, Tomomi Kono, Jie-Yu Chen, Han Zhang
    • Organizer
      Japan Society for Food Engineering
    • Related Report
      2018 Annual Research Report

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Published: 2017-11-13   Modified: 2024-03-26  

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