| Budget Amount *help |
¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2018: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2017: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Outline of Final Research Achievements |
A hypersensitive mutation detection system was established from the mouse genome by original complete outbreeding mating and whole genome sequencing. To further develop into a system that can be easily analyzed in parallel for multiple groups, brother-sister mating method and the expanded trio method were compared. At the same time, the effects of the next generation on different genetic backgrounds and long-term exposure to low-dose gamma rays were also tested. As a result, it was found that the natural mutation rate was reduced by 20% in inbred strains derived from Japanese wild mice, which have low carcinogenicity. In addition, low doses of 6 micro-Gy/hour have no effect on fertility even after exposure for 2 years or more, and multiple groups can be easily continuously exposed to long-term constant mating without homozygosity of newly induced mutations. An expanded outbreeding mating method was finally established for basic as well as applied life sciences.
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