|Budget Amount *help
¥43,810,000 (Direct Cost: ¥33,700,000、Indirect Cost: ¥10,110,000)
Fiscal Year 2020: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2019: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2018: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2017: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
|Outline of Final Research Achievements
The efficient transfer of intermediates from one enzyme to the other by spatial organization of enzyme molecules, as found in the compartments, is believed to be the key feature for well-organized flow of metabolites. The protein assembly on DNA scaffold is an ideal system to study the reaction of enzymes in various arrangements. We have developed a modular adaptor to locate an enzyme of interest at the specific position of DNA scaffold. By taking advantages of the highly orthogonal reaction of our modular adaptors, enzymes were successfully located in defined spatial arrangements on 2D or 3D DNA nanoscaffolds to construct artificial metabolic pathways. Such artificial systems enabled us to discover a novel mechanism to enhance the catalytic activity of enzyme. The artificial metabolic pathway on DNA nanoscaffold was encapsulated in nanoliposome. such a new system accelerates further applications of spatially well-defined enzyme assemblies in bioenergy systems and diagnostics.