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Diversity of polysaccharide-excreting bacteria as carriers of human noroviruses in water environments

Research Project

Project/Area Number 17H01299
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionTohoku University

Principal Investigator

Sano Daisuke  東北大学, 環境科学研究科, 准教授 (80550368)

Co-Investigator(Kenkyū-buntansha) 北島 正章  北海道大学, 工学研究院, 助教 (30777967)
片山 和彦  北里大学, 感染制御科学府, 教授 (60342903)
大村 達夫  東北大学, 工学研究科, 教授 (30111248)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2020: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2019: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2018: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2017: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Keywordsノロウイルス / 血液型決定抗原 / HBGA分泌細菌 / 水環境中動態 / 水環境中動態解明 / 組織血液型決定抗原 / ノロウイルス吸着性細菌 / ノロウイルス吸着細菌
Outline of Final Research Achievements

Infectious disease caused by norovirus is occurring all over the world. In order to control norovirus infectious diseases for which there is no vaccine or medicine, a system that properly combines sanitary engineering technologies such as disinfection based on the life cycle of norovirus needs to be established. As a key to understanding the life cycle of norovirus, we have identified norovirus-binding bacteria that produce histo-blood group antigen (HBGA) -like substance. The purpose of this study was to elucidate the existence of HBGA-secreting bacteria in the aquatic environment and to evaluate the effect of norovirus on the aquatic dynamics of norovirus. A total of 63 HBGA-secreting bacteria were isolated from environmental water, and transposon mutagenesis, gene expression analysis, and Keio collection strains were used to identify genes involved in HBGA secretion. As a result, genes related to the metabolism of galactose were identified as HBGA secretion-related genes.

Academic Significance and Societal Importance of the Research Achievements

本研究により、腸内細菌が有する特定の遺伝子がノロウイルス吸着特性を決定する血液型決定抗原分泌に大きく関わっていることが明らかとなった。血液型決定抗原分泌細菌は、ノロウイルスを物理的に捕捉することで太陽光や消毒などの外的な不活化要因からノロウイルスを守っているものと考えられる。本研究で同定したgalU遺伝子等の血液型決定抗原分泌関連遺伝子をマーカーとして用いることで、水環境や水処理過程における血液型決定抗原分泌細菌の量を把握することができれば、水環境中での自然死滅速度や水処理過程における消毒効率を予測することが可能となり、より安全な水利用を実現することが可能であると考えられる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (28 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Open Access: 3 results) Presentation (16 results) (of which Int'l Joint Research: 9 results,  Invited: 3 results) Book (1 results)

  • [Int'l Joint Research] イリノイ大学アーバナシャンペーン校(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] イリノイ大学(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] The effect of GD1a ganglioside-expressing bacterial strains on murine norovirus infectivity2020

    • Author(s)
      Yifan Zhu, Hiroki Kawai, Satoshi Hashiba, Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano
    • Journal Title

      Molecules

      Volume: 25 Issue: 18 Pages: 4084-4084

    • DOI

      10.3390/molecules25184084

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of novel norovirus polymerase genotypes from pediatric fecal samples collected between the year 1997 and 2000 in Japan2020

    • Author(s)
      Mohan Amarasiri, Etsuko Utagawa, Daisuke Sano, Kazuhiko Katayama
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 82 Pages: 104313-104313

    • DOI

      10.1016/j.meegid.2020.104313

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Required chlorination doses to fulfill the credit value for disinfection of enteric viruses in water: A critical review2020

    • Author(s)
      Andri Rachmadi, Masaaki Kitajima, Tsuyoshi Kato, Hiroyuki Kato, Satoshi Okabe, Daisuke Sano
    • Journal Title

      Environmental Science and Technology

      Volume: 54 Issue: 4 Pages: 2068-2077

    • DOI

      10.1021/acs.est.9b01685

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fecal source tracking in a wastewater treatment and reclamation system using membrane bioreactor evidenced by molecular epidemiology of multiple waterborne gastroenteritis viruses2019

    • Author(s)
      Zheng Ji, Xiaochang C. Wang, Limei Xu, Chongmiao Zhang, Cheng Rong, Andri Taruna Rachmadi, Mohan Amarasiri, Satoshi Okabe, Naoyuki Funamizu, Daisuke Sano
    • Journal Title

      Pathogens

      Volume: 8 Issue: 4 Pages: 170-170

    • DOI

      10.3390/pathogens8040170

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Specific interactions between human norovirus and environmental matrices: Effects on the virus ecology2019

    • Author(s)
      Mohan Amarasiri, Daisuke Sano
    • Journal Title

      Viruses

      Volume: 11 Issue: 3 Pages: 224-224

    • DOI

      10.3390/v11030224

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Specific interactions of rotavirus HAL1166 with Enterobacter cloacae SENG-6 and their contribution on rotavirus HAL1166 removal2019

    • Author(s)
      Mohan Amarasiri, Hiroki Kawai, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano
    • Journal Title

      Water Science and Technology

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reverse transcription-quantitative PCR assays for genotype-specific detection of human noroviruses in clinical and environmental samples2018

    • Author(s)
      Mohan Amarasiri, Masaaki Kitajima, Akiho Miyamura, Ricardo Santos, Silvia Nunes Monteiro, Takayuki Miura, Shinobu Kazama, Satoshi Okabe, Daisuke Sano
    • Journal Title

      International Journal of Hygiene and Environmental Health

      Volume: 221 Issue: 3 Pages: 578-585

    • DOI

      10.1016/j.ijheh.2018.02.008

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Virus particle detection by convolutional neural network in transmission electron microscopy images2018

    • Author(s)
      Eisuke Ito, Takaaki Sato, Daisuke Sano, Etsuko Utagawa, Tsuyoshi Kato
    • Journal Title

      Food and Environmental Virology

      Volume: 10 Issue: 2 Pages: 201-208

    • DOI

      10.1007/s12560-018-9335-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacteriophage removal efficiency as a monitoring tool for virus log reduction in the operation of wastewater reclamation: Review2017

    • Author(s)
      Mohan Amarasiri, Masaaki Kitajima, Thanh H. Nguyen, Satoshi Okabe, Daisuke Sano
    • Journal Title

      Water Research

      Volume: 121 Pages: 258-269

    • DOI

      10.1016/j.watres.2017.05.035

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 健康を支える土木2020

    • Author(s)
      佐野大輔
    • Organizer
      土木の日イベントinぐんま記念講演
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Natural inactivation kinetics modeling of a waterborne enteric virus in surface water by sparse regression and hierarchical Bayesian estimation2020

    • Author(s)
      Wakana Oishi, Ikuo Kato, Osamu Nishimura, Daisuke Sano
    • Organizer
      Water Environment and Technology Conference 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gastroenteritis patient prediction based on wastewater-based epidemiology and dynamic modeling2020

    • Author(s)
      Yifan Zhu, Wakana Oishi, Chikako Maruo, Masaaki Kitajima, Daisuke Sano
    • Organizer
      Water Environment and Technology Conference 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Risk management of waterborne infectious diseases in the SDG era2019

    • Author(s)
      Daisuke Sano
    • Organizer
      Tohoku University Environmental Studies Seminar 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 水インフラとノロウイルス進化2019

    • Author(s)
      佐野大輔
    • Organizer
      第18回ウイルス学夏の学校「みちのくウイルス塾」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Impact of Water, Sanitation, and Hygiene (WASH) interventions on thebacterial pathogen load in households in rural Nepal2019

    • Author(s)
      Sital Uprety, Mohan Amarasiri, Bipin Dangol, Daisuke Sano, Thanh H. Nguyen
    • Organizer
      20th IWA Symposium on Health Related Water Microbiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Predictive Water Microbiology: Hierarchical Bayesian Modelling for Forecasting Virus Disinfection Efficiency2019

    • Author(s)
      Syun-suke Kadoya, Osamu Nishimura, Hiroyuki Kato, Daisuke Sano
    • Organizer
      WEF-EESS Conference on Advancement in Water and Wastewater Treatment and Reuse
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Water Science and Technology2018

    • Author(s)
      Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano
    • Organizer
      IWA World Water Congress & Exhibition 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of bacterial genes for producing norovirus-binding glycoconjugate2018

    • Author(s)
      Mohan Amarasiri, Peiyi Yang, Masaaki Kitajima, Yasuhiro Kasahara, Satoshi Okabe, Daisuke Sano
    • Organizer
      6th ISFEV Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 腸内細菌の組織血液型決定抗原様物質分泌関連遺伝子に関する研究2018

    • Author(s)
      小島友祐、モハン・アマラシリ、佐野大輔
    • Organizer
      平成29年度土木学会東北支部技術研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 共存細菌との結合が水系感染症ウイルスの感染効率に与える影響2018

    • Author(s)
      河合大樹、北島正章、岡部聡、佐野大輔
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Electrochemical and colorimetric DNA aptasensor technologies for rapid norovirus detection2017

    • Author(s)
      Masaaki Kitajima, Seiya Hirano, Akihiro Okamoto, Daisuke Sano, Hisashi Satoh, Satoshi Okabe
    • Organizer
      19th International Symposium on Health-Related Water Microbiology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Removal of rotavirus by microfiltration in the presence of HBGA-positive bacteria2017

    • Author(s)
      Mohan Amarasiri, Hiroki Kawai, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano
    • Organizer
      第4回環境水質工学シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Histo-blood group antigen (HBGA) positive bacteria in the mixed liquor reduces the human rotavirus concentration in membrane filtration effluent2017

    • Author(s)
      Mohan Amarasiri, Hiroki Kawai, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano
    • Organizer
      Water and Environment Technology Conference 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Human enteric virus removal from wastewater: Design and operational monitoring of multiplebarrier system and virus removal efficiency improvement using specific interaction2017

    • Author(s)
      Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe, Daisuke Sano
    • Organizer
      第20回日本水環境学会シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] DNAアプタマーを用いた簡易ノロウイルス検出法の開発2017

    • Author(s)
      吉原光、北島正章、佐野大輔、岡部聡、高橋正宏、佐藤久
    • Organizer
      平成29年度全国会議(水道研究発表会)
    • Related Report
      2017 Annual Research Report
  • [Book] Aptamer: The Potential Application to Norovirus Research, Diagnosis, and Therapeutics, Noroviruses: Outbreaks, Control and Prevention Strategies2017

    • Author(s)
      Seiya Hirano, Daisuke Sano, Satoshi Okabe, Masaaki Kitajima
    • Total Pages
      16
    • Publisher
      Nova Science Publisher
    • ISBN
      9781536119596
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-02-28  

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