|Budget Amount *help
¥42,120,000 (Direct Cost: ¥32,400,000、Indirect Cost: ¥9,720,000)
Fiscal Year 2020: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2019: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2018: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2017: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
|Outline of Final Research Achievements
We established a culture system to induce primordial germ cell-like cells (cj-PGCLCs) from common marmoset ES cells. The transcriptomic and epigenetic features of cj-PGCLCs were quite similar to those of human PGCLCs. Based on the transcriptome analysis, we identified surface marker antigens that are specifically expressed on cj-PGCLCs. By recognizing these antigens with antibodies, we were able to isolate cj-PGCLCs from a variety of common marmoset ES cell lines. The obtained cj-PGCLCs were differentiated into the late germ cell lineage by co-culture with mouse embryonic ovarian somatic cells, suggesting that they have the ability to differentiate into gametes.