Light-mediated regulation of flagellar and ciliary motility by a novel photoreceptor protein

• Project/Area Number: 17H01440
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: University of Tsukuba
• Principal Investigator: Inaba Kazuo 筑波大学, 生命環境系, 教授 (80221779)
• Co-Investigator(Kenkyū-buntansha): 竹田 扇 山梨大学, 大学院総合研究部, 教授 (20272429) ; 昆 隆英 大阪大学, 理学研究科, 教授 (30332620) ; 若林 憲一 東京工業大学, 科学技術創成研究院, 准教授 (80420248) ; 広瀬 恵子 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (90357872)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥33,930,000 (Direct Cost: ¥26,100,000、Indirect Cost: ¥7,830,000); Fiscal Year 2019: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2018: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2017: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: ダイニン / 鞭毛運動 / 繊毛運動 / DYBLUP / 光受容タンパク質 / クラミドモナス / 精子 / 走光性 / 精子鞭毛 / 光受容 / BLUF / cilia / flagella / axoneme / sperm / light / 線毛運動

Outline of Final Research Achievements

This research project was aimed to clarify the structure and regulatory function of DYBLUP, which is a novel axonemal protein containing a blue-light using FAD (BLUF) domain and is known to be associated with an axonemal dynein. During three years research periods, our group have shown that DYBLUP is associated with both the motor domain of beta heavy chain of an inner arm dynein f/I1 and the tether structure on the A-tubule of a doublet microtubule. Using a Chlamydomonas mutant, we have further shown that DYPLUP plays an essential role in avoidance of the acclimation to strong blue-light by retaining negative phototaxis.

Academic Significance and Societal Importance of the Research Achievements

生物の光応答は、代謝や繁殖行動、生態など、幅広い生命活動に重要である。多くの生物において、過剰な青色光は細胞に毒性を示すことが知られている。本研究は、光に反応し鞭毛繊毛運動を制御する新規タンパク質DYBLUPの構造と機能の一端を明らかにした。鞭毛や繊毛は、単細胞生物からヒトまで進化的に高く保存されてきた構造である。本研究の成果は、生態系からヒト疾患まで、鞭毛繊毛が司どる多様な生命現象の光制御に重要な知見をもたらすと考えられる。また、光による分子モーターの直接制御という医学、工学分野の応用研究を開拓する新たな道を開くきっかけとなることが期待される。

Research Products

• [Int'l Joint Research] スイス連邦工科大学(スイス)
• [Int'l Joint Research] CNRS(フランス)
• [Journal Article] Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice (2020)
  • Author(s): Morohoshi A., Miyata H., Shimada K., Nozawa K., Matsumura T., Yanase R., Shiba K., Inaba K., Ikawa M.
  • Journal Title: PLoS Genetics Volume: 16 Pages: 1008585-1008585
  • DOI: 10.1371/journal.pgen.1008585
  • Peer Reviewed / Open Access
• [Journal Article] Tree of motility A proposed history of motility systems in the tree of life (2020)
  • Author(s): M Miyata, R C Robinson, T QP Uyeda, Y Fukumori, S Fukushima, S Haruta, M Homma, K Inaba, M Ito, C Kaito, K Kato, TKenri, Y Kinosita, S Kojima, T Minamino, H Mori, S Nakamura, D Nakane, K Nakayama, M Nishiyama, S Shibata, K Shimabukuro, M Tamakoshi, A Taoka, Y Tashiro, ITulum, HiWada, K Wakabayashi
  • Journal Title: Genes Cells Volume: 25 Pages: 6-21
  • DOI: 10.1111/gtc.12737
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CTENO64 Is Required for Coordinated Paddling of Ciliary Comb Plate in Ctenophores (2019)
  • Author(s): Jokura Kei、Shibata Daisuke、Yamaguchi Katsushi、Shiba Kogiku、Makino Yumiko、Shigenobu Shuji、Inaba Kazuo
  • Journal Title: Curr Biol Volume: 29 Pages: 3510-3516
  • DOI: 10.1016/j.cub.2019.08.059
  • Peer Reviewed / Open Access
• [Journal Article] Calaxin is required for cilia-driven determination of vertebrate laterality (2019)
  • Author(s): Sasaki Keita、Shiba Kogiku、Nakamura Akihiro、Kawano Natsuko、Satouh Yuhkoh、Yamaguchi Hiroshi、Morikawa Motohiro、Shibata Daisuke、Yanase Ryuji、Jokura Kei、Nomura Mami、Miyado Mami、Takada Shuji、Ueno Hironori、Nonaka Shigenori、Baba Tadashi、Ikawa Masahito、Kikkawa Masahide、Miyado Kenji、Inaba Kazuo
  • Journal Title: Commun Biol Volume: 2 Pages: 1-11
  • DOI: 10.1038/s42003-019-0462-y
  • Peer Reviewed / Open Access
• [Journal Article] Single-cell genomics unveiled a cryptic cyanobacterial lineage with a worldwide distribution hidden by a dinoflagellate host (2019)
  • Author(s): Nakayama T., Nomura M., Takano Y., Tanifuji G., Shiba K., Inaba K., Inagaki Y., Kawata M.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 116 Pages: 15973-15978
  • DOI: 10.1073/pnas.1902538116
  • Peer Reviewed / Open Access
• [Journal Article] A brown algal sex pheromone reverses the sign of phototaxis by cAMP/Ca2+-dependent signaling in the male gametes of Mutimo cylindricus (Cutleriaceae). (2019)
  • Author(s): Nana Kinoshita-Terauchi, Kogiku Shiba, Taiki Umezawa, Fuyuhiko Matsuda, Taizo Motomura, Kazuo Inaba
  • Journal Title: J Photochem Photobiol B. Volume: 192 Pages: 113-123
  • DOI: 10.1016/j.jphotobiol.2019.01.010
  • Peer Reviewed
• [Journal Article] Microtubule stabilizer reveals requirement of Ca2+-dependent conformational changes of microtubules for rapid coiling of haptonema in haptophyte algae. (2019)
  • Author(s): Nomura Mami、Atsuji Kohei、Hirose Keiko、Shiba Kogiku、Yanase Ryuji、Nakayama Takeshi、Ishida Ken-ichiro、Inaba Kazuo
  • Journal Title: Biol Open Volume: 8 Pages: 036590-036590
  • DOI: 10.1242/bio.036590
  • NAID: 120006632794
  • Peer Reviewed / Open Access
• [Journal Article] Ca2+ efflux via plasma membrane Ca2+-ATPase mediates chemotaxis in ascidian sperm. (2018)
  • Author(s): Yoshida Kaoru, Shiba Kogiku, Nakamoto Ayako, Ikenaga Jumpei, Matsunaga Shigeru, Inaba Kazuo, Yoshida Manabu
  • Journal Title: Sci Rep Volume: 8 Pages: 16622-16622
  • DOI: 10.1038/s41598-018-35013-2
  • NAID: 120006714285
  • Peer Reviewed / Open Access
• [Journal Article] Release of sticky glycoproteins from Chlamydomonas flagella during microsphere translocation on the surface membrane. (2018)
  • Author(s): Kamiya Ritsu, Shiba Kogiku, Inaba Kazuo, Kato-Minoura Takako
  • Journal Title: Zoolog Sci Volume: 35 Pages: 299-305
  • DOI: 10.2108/zs180025
  • NAID: 40021626738
  • Peer Reviewed
• [Journal Article] Microscopic analysis of sperm movement : links to mechanisms and protein components (2018)
  • Author(s): Inaba K., Shiba K.
  • Journal Title: Microscopy (Oxf) Volume: 67 Pages: 144-155
  • DOI: 10.1093/jmicro/dfy021
  • Peer Reviewed
• [Journal Article] Inverse relationship of Ca2+-dependent flagellar response between animal sperm and prasinophyte algae. (2017)
  • Author(s): Shiba Kogiku、Inaba Kazuo
  • Journal Title: J Plant Res Volume: 130 Pages: 465-473
  • DOI: 10.1007/s10265-017-0931-7
  • Peer Reviewed
• [Journal Article] Mizuno K, Shiba K, Yaguchi J, Shibata D, Yaguchi S, Pruliere G, Chenevert J, Inaba K. (2017)
  • Author(s): Mizuno Katsutoshi、Shiba Kogiku、Yaguchi Junko、Shibata Daisuke、Yaguchi Shunsuke、Pruliere Gerard、Chenevert Janet、Inaba Kazuo
  • Journal Title: Scientific Reports Volume: 7 Pages: 10751-10751
  • DOI: 10.1038/s41598-017-10822-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chlamydomonas DYX1C1/PF23 is essential for axonemal assembly and proper morphology of inner dynein arms. (2017)
  • Author(s): Yamamoto, R., Obbineni, J.M., Alford, L.M., Ide, T., Owa, M., Hwang, J., Kon, T., Inaba, K., James, N., King, S.M., Ishikawa, T., Sale, W.S., Dutcher, S.K.
  • Journal Title: PLOS Genetics Volume: 13
  • DOI: 10.1371/journal.pgen.1006996
  • NAID: 120006355802
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Microtubule dynamics in the haptonema of haptophyte algae (2019)
  • Author(s): Ryuji Yanase, Mami Nomura, Kogiku Shiba, Kazuo Inaba
  • Organizer: VIII European Congress of Protistology & ISOP Joint meeting
  • Int'l Joint Research
• [Presentation] カブトクラゲ櫛板を構成するCTENO189の同定 (2019)
  • Author(s): 城倉 圭，柴 小菊，稲葉 一男
  • Organizer: 日本動物学会第90回大阪大会
• [Presentation] Structural/functional analyses on MOT7, a novel light chain of ciliary dynein f/I1 (2019)
  • Author(s): 山本 遼介, 中桐 侑平, 久冨 理, 今井 洋, 宋 致, 村田 和義, 若林 憲一, 石川 尚, 稲葉 一男, 昆 隆英
  • Organizer: 第57回日本物理学会年会
• [Presentation] Synchrotron radiation X-ray diffractionv reveals the highly ordered structure of axonemes in the comb plate of ctenophores (2019)
  • Author(s): 岩本 裕之, 城倉 圭, 稲葉 一男
  • Organizer: 第57回日本物理学会年会
• [Presentation] cAMPとカルシウムイオンによる微小管滑り運動の協調的制御 (2019)
  • Author(s): 山口 さやか，柴 小菊，稲葉 一男
  • Organizer: 日本動物学会第90回大阪大会
• [Presentation] cAMP modulates Ca2+-mediated regulation of sperm flagellar waveform (2018)
  • Author(s): Kogiku Shiba, Taichi Sakano, Toshiaki Furuta, Eiji Fujiwara, Shoji A Baba, Kazuo Inaba
  • Organizer: XIII International Symposium on Spermatology
  • Int'l Joint Research
• [Presentation] Calaxin is essential for ciliary formation in nodal monocilia but not in sperm flagella or epithelial multicilia (2018)
  • Author(s): Keita Sasaki, Kogiku Shiba, Akihiro Nakamura, Natsuko Kawano, Yuhkoh Satouh, Daisuke Shibata, Mami Miyado, Shuji Takada, Hironori Ueno, Shigenori Nonaka, Masahito Ikawa, Tadashi Baba, Kenji Miyado, Kazuo Inaba
  • Organizer: XIII International Symposium on Spermatology
  • Int'l Joint Research
• [Presentation] cAMP modulates Ca2+-mediated regulation of sperm flagellar waveform (2018)
  • Author(s): Kogiku Shiba, Taichi Sakano, Toshiaki Furuta, Eiji Fujiwara, Shoji A. Baba, Kazuo Inaba
  • Organizer: 第56回日本生物物理学会年会
• [Presentation] Identification of a novel protein CTENO64 in giant compound cilia in the ctenophore and its role in the coordinated ciliary movement (2018)
  • Author(s): Shibata, Katsushi Yamaguchi, Shuji Shigenobu, Kogiku Shiba, Kazuo Inaba
  • Organizer: 第56回日本生物物理学会年会
• [Presentation] The comb plate of ctenophore is a nearly perfect giant single protein crystal that moves (2018)
  • Author(s): Hiroyuki Iwamoto, Kei Jokura, Kazuo Inaba
  • Organizer: 第56回日本生物物理学会年会
• [Presentation] Chemical genetics reveal the role of 14-3-3 epsilon a in Ciona notochord tubulogenesis (2017)
  • Author(s): Yuji Mizotani, Mayu Suzuki, Hidenori Watanabe, Kogiku Shiba, Kazuo Inaba, Kohji Hotta, Kotaro Oka, Etsu Tashiro, Masaya Imoto
  • Organizer: International Tunicate Meeting
  • Int'l Joint Research
• [Presentation] Effect of sex pheromone on the sign of phototaxis in brown algal male gametes (2017)
  • Author(s): Kinoshita, N., Shiba, K., Inaba, K., Umezawa, T., Motomura, T.
  • Organizer: 11th International Phycological Congress
  • Int'l Joint Research
• [Presentation] カルボニックアンヒドラーゼを介したカレイ類精子運動のCO2 阻害効果：種間比較解析 (2017)
  • Author(s): 佐野 マリコ，柴 小菊，稲葉 一男
  • Organizer: 日本動物学会第88回大会
• [Presentation] カルシウムイオンを介した精子鞭毛波形制御におけるcAMP の調整作用 (2017)
  • Author(s): 柴 小菊，坂野 太一，古田 寿昭，藤原 英史，馬場 昭次，稲葉 一男
  • Organizer: 日本動物学会第88回大会
• [Presentation] Microtubule dynamics for rapid coiling movement of haptonema in the haptophyte algae. ハプト藻に存在するハプトネマの急速コイリング運動における微小管ダイナミクス (2017)
  • Author(s): 野村 真未, 阿閉 耕平, 広瀬 恵子, 柴 小菊, 稲葉 一男
  • Organizer: “メカニカルコミュニケーションが生み出す生体運動の多様性”, 第55回日本生物物理学会年会
  • Invited
• [Presentation] カブトクラゲ櫛板を構成する新規タンパク質の同定 (2017)
  • Author(s): 城倉圭, 柴田大輔, 山口勝司, 柴小菊, 重信秀治, 稲葉一男
  • Organizer: 第8回繊毛研究会
• [Presentation] Light-responsive regulation of ciliary motility by a novel subunit of axonemal dynein with a photoreceptor protein (2017)
  • Author(s): Kazuo Inaba
  • Organizer: International Workshop Dynein 2017
  • Int'l Joint Research / Invited
• [Presentation] cAMP modulates Ca2+-mediated regulation of sperm flagellar waveform (2017)
  • Author(s): Kogiku Shiba, Taichi Sakano, Toshiaki Furuta, Eiji Fujiwara, Shoji A Baba, Kazuo Inaba
  • Organizer: International Workshop Dynein 2017
  • Int'l Joint Research
• [Presentation] CTENO64 is a SMC-domain containing protein that connects multiple axonemes in the comb plates of ctenophore (2017)
  • Author(s): Kei Jokura, Daisuke Shibata, Katsushi Yamaguchi, Shuji Shigenobu, Yumiko Makino, Kogiku Shiba, Kazuo Inaba
  • Organizer: International Workshop Dynein 2017
  • Int'l Joint Research
• [Book] Handbook of Dynein (2019)
  • Author(s): Kazuo Inaba (Editor, K Hirose)
  • Publisher: CRC Press
  • ISBN: 9789814800013
• [Remarks] 筑波大学下田臨海実験センター稲葉研究室
  • URL: http://www.shimoda.tsukuba.ac.jp/~inaba/