|Budget Amount *help
¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000)
Fiscal Year 2020: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2019: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2018: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2017: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
|Outline of Final Research Achievements
This study aimed to clarify the indirect defence mechanism in the plant rhizosphere and identify the target molecules of the defence compounds produced by symbionts for plant protection. The PI purified soybean signals stimulating okaramine biosynthesis in Penicillium simplicissimum, while uncovering the okaramine biosynthesis genes in the fungus. The PI also discovered co-factors enabling functional expression of insect nicotinic acetylcholine receptors (nAChRs). The Co-Is found that fungal products decalins inhibited the Noppera-bo protein underpinning ecdysteroid biosynthesis in the fruit fly Drosophila melanogaster. Metabolome analysis of soils used to cultivate soybean plants after growing hairy vetch led to the finding of okaramines. Some Streptomyces microorganisms produce allosamidin that inhibits chitinases and chitinase like proteins. The Co-I found that allosamidin had activity to promote plant lateral root formation, providing new insight into the indirect defence.