Elucidation of the regulatory mechanism of amino acid metabolism in response to stress

• Project/Area Number: 17H01503
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kyushu University
• Principal Investigator: Furuse Mitsuhiro 九州大学, 農学研究院, 教授 (30209176)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000); Fiscal Year 2019: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2017: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
• Keywords: ストレス / アミノ酸 / ニューロペプチドY / 乳汁 / ヒナ / 体温 / 脳 / 代謝 / アルギニン / シトルリン / オルニチン / 催眠作用 / 神経ペプチド / 消化管 / 筋肉 / 暑熱ストレス / ゴナドトロピン抑制ホルモン / 酸化ストレス / ポリフェノール / ストレッサー / 行動 / 拘束ストレス / D-アルギニン / L-ロイシン / タウリン / アンセリン / ミルク / 脳・神経 / 栄養学 / 畜産学 / 生体機能利用

Outline of Final Research Achievements

The effect of suppressing the increase in body temperature was confirmed by oral administration of the live bacterial culture medium containing L-citrulline or the watermelon rind rich in L-citrulline. Central administration of neuropeptide Y increased plasma taurine and anserine concentrations under high environmental temperature. In ovo injection of L-leucine into broiler eggs imparted heat resistance to male chicks after hatching, and attenuated elevation of arginine in diencephalon and liver due to high environmental temperature.
Restraint stress on lactating mother mice promoted the transfer of taurine into milk. Dietary L-serine increased free L-serine levels in milk. Oral administration of D-arginine rapidly transferred to the cerebral cortex, hypothalamus, and milk. L-Ornithine was rapidly metabolized to L-citrulline, increasing the levels of L-proline and glycine, which are important components of collagen, in the skin.

Academic Significance and Societal Importance of the Research Achievements

優秀な遺伝子を選抜し、栄養的に優れた飼料を与えたとしても家畜・家禽は十分にその能力を発揮できないことがある。その一因がストレスであり、軽減のために飼育環境の改善が求められている。特に幼い動物はストレス負荷の影響を受けやすく、施設などの改善に留まらず、動物の内面からのストレス軽減方法を見出すことは学術的に意義がある。そこでアミノ酸を用いて検討した。
社会に及ぼす影響としては、暑熱ストレスによる負荷が増大する中、化石燃料を用いて施設の環境温度を下げるのではなくアミノ酸を用いることにより体温上昇を軽減できることを示した。また、乳汁の遊離アミノ酸の濃度の制御が可能であることも認めた。

Research Products

• [Journal Article] Oral Administration of Watermelon Rind Extract to Induce Hypothermia in Chicks (2020)
  • Author(s): Nguyen Linh T.N.、Eltahan Hatem M.、Pham Cuong V.、Han Guofeng、Chowdhury Vishwajit S.、Furuse Mitsuhiro
  • Journal Title: J. Poult. Sci. Volume: 57 Issue: 1 Pages: 37-44
  • DOI: 10.2141/jpsa.0190054
  • NAID: 130007792223
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Intracerebroventricular Injection of L-Pipecolic Acid Exerts Hypnotic Effects Without Activating NMDA Receptors in Neonatal Chicks under Social Isolation-induced Stress (2020)
  • Author(s): Shigemura, A., Chowdhury, V.S. and Furuse, M.
  • Journal Title: J. Poult. Sci. Volume: 57 Issue: 1 Pages: 84-87
  • DOI: 10.2141/jpsa.0190067
  • NAID: 130007792193
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Open Access
• [Journal Article] Orally administrated D-arginine exhibits higher enrichment in the brain and milk than L-arginine in ICR mice (2020)
  • Author(s): Aso, K., Nishigawa, T., Nagamachi, S., Takakura, M. and Furuse, M.
  • Journal Title: J. Vet. Med. Sci. Volume: 82 Issue: 3 Pages: 307-313
  • DOI: 10.1292/jvms.19-0630
  • NAID: 130007805850
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Nutritional Characteristics and Functions of D-Amino Acids in the Chicken (2020)
  • Author(s): Tomonaga, S. and Furuse, M.
  • Journal Title: J. Poult. Sci. Volume: 57 Issue: 1 Pages: 18-27
  • DOI: 10.2141/jpsa.0190062
  • NAID: 130007792232
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Open Access
• [Journal Article] Oral administration of L-ornithine increases the content of both collagen constituting amino acids and polyamines in mouse skin (2019)
  • Author(s): Harada, D., Nagamachi, S., Aso, K., Ikeda, K., Takahasi, Y. and Furuse, M.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 81 Pages: 1-11
  • DOI: 10.1016/j.bbrc.2019.03.147
  • Peer Reviewed
• [Journal Article] Decreased stress-induced depression-like behavior in lactating rats is associated with changes in the hypothalamic-pituitary-adrenal axis, brain monoamines, and brain amino acid metabolism (2019)
  • Author(s): Hamada, M., Nishigawa, T., Maesono, S., Aso, K., Ikeda, H. and Furuse, M.
  • Journal Title: Stress Volume: 22 Pages: 482-491
  • DOI: 10.1080/10253890.2019.1584179
  • Peer Reviewed
• [Journal Article] Differences in free amino acid concentrations in milk between Wistar and Wistar Kyoto rats (2019)
  • Author(s): Hamada, M., Ihara, T. and Furuse, M.
  • Journal Title: J. Vet. Med. Sci. Volume: 81 Issue: 6 Pages: 838-845
  • DOI: 10.1292/jvms.19-0064
  • NAID: 130007660449
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Effects of oral administration of yoghurt whey on free amino acid concentration in rat brains (2019)
  • Author(s): Kawase, T. and Furuse, M.
  • Journal Title: Journal of Pet Animal Nutrition Volume: 22 Issue: 2 Pages: 84-94
  • DOI: 10.11266/jpan.22.2_84
  • NAID: 130007739736
  • ISSN: 1344-3763, 2185-7601
  • Peer Reviewed / Open Access
• [Journal Article] Oral Administration of a Medium Containing L-Citrulline-producing Live Bacteria Reduces Body Temperature in Chicks (2019)
  • Author(s): Tran Phuong V.、Do Phong H.、Han Guofeng、Bahry Mohammad A.、Yang Hui、Chowdhury Vishwajit S.、Furuse Mitsuhiro
  • Journal Title: J. Poult. Sci. Volume: 56 Issue: 4 Pages: 285-289
  • DOI: 10.2141/jpsa.0180136
  • NAID: 130007733393
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed / Open Access
• [Journal Article] Central regulation of feeding behavior through neuropeptides and amino acids in neonatal chicks (2019)
  • Author(s): Tran, P.V., Chowdhury, V.S. and Furuse, M.
  • Journal Title: Amino Acids Volume: 51 Pages: 1129-1152
  • DOI: 10.1007/s00726-019-02762-x
  • Peer Reviewed
• [Journal Article] Effects of in ovo feeding of L-leucine on amino acids metabolism and heat-shock protein-70, and -90 mRNA expression in heat-exposed chicks. (2019)
  • Author(s): Han, G., Yang, H., Ikeda, H., Wang, Y., Zhang, R., Tashiro, K., Bungo, T., Furuse, M. and Chowdhury, V.S.
  • Journal Title: Poultry Science Volume: 98 Pages: 1243-1253
  • DOI: 10.3382/ps/pey444
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Flavangenol regulates gene expression of HSPs, anti-apoptotic and anti-oxidative factors to protect primary chick brain cells exposed to high temperature (2019)
  • Author(s): Yang H, Chowdhury VS, Han G, Zhang R, Furuse M.
  • Journal Title: Journal of Thermal Biology Volume: 81 Pages: 1-11
  • DOI: 10.1016/j.jtherbio.2019.02.010
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reduction in voluntary food intake, but not fasting, stimulates hypothalamic gonadotropin-inhibitory hormone precursor mRNA expression in chicks under heat stress (2018)
  • Author(s): Bahry MA, Yang H, Tran PV, Do PH, Han G, Eltahan HM, Chowdhury VS, Furuse M.
  • Journal Title: Neuropeptides Volume: 71 Pages: 90-96
  • DOI: 10.1016/j.npep.2018.09.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Taurine and β-alanine intraperitoneal injection in lactating mice modifies the growth and behavior of offspring (2018)
  • Author(s): Nishigawa Takuma、Nagamachi Satsuki、Chowdhury Vishwajit S.、Yasuo Shinobu、Furuse Mitsuhiro
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Pages: 2024-2029
  • DOI: 10.1016/j.bbrc.2017.12.063
  • Peer Reviewed
• [Journal Article] In ovo L -leucine administration stimulates lipid metabolisms in heat-exposed male, but not female, chicks to afford thermotolerance (2018)
  • Author(s): Han Guofeng、Yang Hui、Bungo Takashi、Ikeda Hiromi、Wang Yunhao、Nguyen Linh T.N.、Eltahan Hatem M.、Furuse Mitsuhiro、Chowdhury Vishwajit S.
  • Journal Title: Journal of Thermal Biology Volume: 71 Pages: 74-82
  • DOI: 10.1016/j.jtherbio.2017.10.020
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dietary L-serine modifies free amino acid composition of maternal milk and lowers the body weight of the offspring in mice (2018)
  • Author(s): NAGAMACHI Satsuki、NISHIGAWA Takuma、TAKAKURA Mayumi、IKEDA Hiromi、KODAIRA Momoko、YAMAGUCHI Takeshi、CHOWDHURY Vishwajit Sur、YASUO Shinobu、FURUSE Mitsuhiro
  • Journal Title: J. Vet. Med. Sci. Volume: 80 Issue: 2 Pages: 235-241
  • DOI: 10.1292/jvms.17-0577
  • NAID: 130006339622
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Restraint stress in lactating mice alters the levels of sulfur-containing amino acids in milk (2018)
  • Author(s): NISHIGAWA Takuma、NAGAMACHI Satsuki、IKEDA Hiromi、CHOWDHURY Vishwajit S.、FURUSE Mitsuhiro
  • Journal Title: J. Vet. Med. Sci. Volume: 80 Issue: 3 Pages: 503-509
  • DOI: 10.1292/jvms.17-0661
  • NAID: 130006588561
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Central NPY-Y5 sub-receptor partially functions as a mediator of NPY-induced hypothermia and affords thermotolerance in heat-exposed fasted chicks (2017)
  • Author(s): Eltahan Hatem M.、Bahry Mohammad A.、Yang Hui、Han Guofeng、Nguyen Linh T. N.、Ikeda Hiromi、Ali Mohamed N.、Amber Khairy A.、Furuse Mitsuhiro、Chowdhury Vishwajit S.
  • Journal Title: Physiological Reports Volume: 5
  • DOI: 10.14814/phy2.13511
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 母マウスへの D-アルギニンまたは D-セリン投与が仔マウスの成長および行動に及ぼす影響 (2019)
  • Author(s): 麻生 賢太，池田 和輝，原田 大暉，古瀬 充宏
  • Organizer: 日本アミノ酸学会第13回学術大会
• [Presentation] 急性ストレスが皮膚のアミノ酸代謝に及ぼす影響 (2019)
  • Author(s): 早川 佳歩，麻生 賢太，池田 和輝，原田 大暉，古瀬 充宏
  • Organizer: 日本アミノ酸学会第13回学術大会
• [Presentation] 骨格筋のアミノ酸代謝に及ぼす β-ヒドロキシβ-メチルブチレート（HMB）の影響 (2019)
  • Author(s): 池田 和輝，原田 大暉，早川 佳歩，古瀬 充宏
  • Organizer: 日本アミノ酸学会第13回学術大会
• [Presentation] 輸送ストレスが黒毛和牛の血漿遊離アミノ酸濃度に及ぼす影響について (2019)
  • Author(s): 尾川寅太，稲田淳，深水大，渡邉美佐江，池田和輝，原田大暉，浜田瑞貴，古瀬充宏
  • Organizer: 第126回日本畜産学会
• [Presentation] L-Leucine stimulates growth in heat-exposed broilers (2019)
  • Author(s): Chowdhury VS, Han G, Bungo T, Tashiro K, Furuse M,
  • Organizer: 第125回日本畜産学会
• [Presentation] Neuropeptide Y plays an anti-stress role in heat-exposed chicks (2018)
  • Author(s): Chowdhury VS, Cockrem JF, Furuse M.
  • Organizer: 8th Intercongress Symposium of the Asia and Oceania Society for Comparative Endcrinology
  • Int'l Joint Research
• [Presentation] Oral administration of L-citrulline affords thermotolerance in heat-exposed broilers (2018)
  • Author(s): Chowdhury VS, Han G, Shiraishi J, Sugino T, Furuse M, Bungo, T.
  • Organizer: 18th AAAP Animal Science Congress
  • Int'l Joint Research
• [Presentation] スイカ外皮抽出物の経口投与はヒナに低体温症をもたらす (2018)
  • Author(s): リン T. N. グェン・ハテム M. エルタハン・クオン V. ファム・韓 国鋒・ スルチードリ ビシュワジット・古瀬 充宏
  • Organizer: 日本家禽学会
• [Presentation] タウリンの中枢投与はヒナに低体温を引き起こすが、経口投与では逆に体温上昇を誘発する (2018)
  • Author(s): ハテム M. エルタハン・韓 国鋒・楊 輝・リン T. N. グェン・ クオン・ヴァン・ファム・古瀬 充宏・スルチードリ ビシュワジット
  • Organizer: 日本家禽学会
• [Presentation] 卵内へのロイシン投与はブロイラーのアミノ酸代謝及び熱ショックタンパク質遺伝子発現量に影響する (2018)
  • Author(s): 韓 国鋒・楊 輝・田代 康介・豊後 貴嗣・古瀬 充宏・スルチードリ ビシュワジット
  • Organizer: 日本家禽学会
• [Presentation] In ovo administration of L-leucine, but not oral or central, improves thermotolerance in chicks (2018)
  • Author(s): Han G, Yang H, Tashiro K, Bungo T, Furuse M, Chowdhury VS
  • Organizer: 日本家禽学会
• [Presentation] Effects of pine bark extract (flavangenol) on the expression of heat-shock proteins and related other genes in heat-exposed chick brain cells (2018)
  • Author(s): Yang H, Han G, Zhang R, Chowdhury VS, Furuse M
  • Organizer: 日本家禽学会
• [Presentation] マウスの肝臓と皮膚におけるＬ-オルニチンの単回経口投与による遊離アミノ酸およびポリアミンの変化 (2018)
  • Author(s): 原田 大暉・長町 さつ・麻生 健太・池田 和輝・高橋 芳弘・安尾 しのぶ・古瀬 充宏
  • Organizer: 日本アミノ酸学会
• [Presentation] 2系統ラット間の妊娠前後の行動および授乳期の血漿・母乳中遊離アミノ酸濃度 (2018)
  • Author(s): 浜田 瑞貴・井原 翼・安尾 しのぶ・古瀬 充宏
  • Organizer: 日本アミノ酸学会
• [Presentation] L-ロイシンの卵内投与は暑熱ストレスによる雄ブロイラー雛のアミノ酸代謝を変化させる (2018)
  • Author(s): 韓 国鋒・楊 輝・田代 康介・豊後 貴司・古瀬 充宏・スルチョードリビシュワジット
  • Organizer: 日本アミノ酸学会
• [Presentation] D-およびL-アルギニンの経口投与が母マウスの乳汁遊離アミノ酸組成に及ぼす影響 (2018)
  • Author(s): 麻生 賢太・長町 さつき・ 安尾 しのぶ・古瀬 充宏
  • Organizer: 日本アミノ酸学会
• [Presentation] 卵内へのロイシン投与は出荷日齢ブロイラーの脂質代謝に影響を与えず熱耐性を誘導する (2018)
  • Author(s): 韓 国鋒・楊 輝・豊後 貴嗣・古瀬 充宏・スルチードリ ビシュワジット
  • Organizer: 日本家禽学会
• [Presentation] Different modes of L-leucine in ovo feeding on embryonic growth and rectal temperature in chicks (2018)
  • Author(s): 韓国鋒・楊輝・池田裕美・豊後貴嗣・古瀬充宏・Sur Chowdhury Vishwajit
  • Organizer: 第124回日本畜産学会
• [Presentation] 授乳期間中の母マウスへのタウリン及びβ‐アラニン投与が仔マウスの初期成長と行動に及ぼす影響 (2017)
  • Author(s): 西川琢磨・長町さつき・浜田瑞貴・池田裕美・スルチョードリ ビシュワジット・安尾 しのぶ・古瀬充宏
  • Organizer: 日本アミノ酸学会
• [Presentation] 妊娠・出産およびストレスが母体の行動やアミノ酸代謝に及ぼす影響 (2017)
  • Author(s): 浜田瑞貴・西川琢磨・前園沙織・麻生賢太・池田裕美・スルチョードリ ビシュワジット・安尾 しのぶ・古瀬 充宏
  • Organizer: 日本アミノ酸学会
• [Presentation] 経口投与によるL-アルギニンおよびD-アルギニンの脳への取り込みについて (2017)
  • Author(s): 麻生賢太・池田裕美・西川琢磨・黒木優美・山本恵理・浅見幸夫・安尾 しのぶ・古瀬 充宏
  • Organizer: 日本アミノ酸学会
• [Presentation] L-ロイシンの卵内投与はブロイラー雛の脂質代謝および耐熱性を変化させる (2017)
  • Author(s): 韓 国鋒・楊 輝・豊後 貴嗣・池田裕美・古瀬 充宏・スルチードリ ビシュワジット
  • Organizer: 日本アミノ酸学会
• [Presentation] L-Leucine in ovo injection stimulates lipid metabolisms and affords thermotolerance in male broiler chicks (2017)
  • Author(s): Han, G., Yang, H., Ikeda, H., Bungo, T., Furuse, M., Chowdhury, V.S.
  • Organizer: 第123回日本畜産学会
• [Presentation] Chronic administration of pine bark extract (flavangenol) attenuates brain HSPs mRNA expressions in in vivo and in vitro (2017)
  • Author(s): Yang, H., Chowdhury, V.S., Han, G., Furuse, M.
  • Organizer: 第123回日本畜産学会
• [Presentation] Maternal stress influences the sulfur amino acid levels in milk and the growth of offspring (2017)
  • Author(s): Nishigawa, T., Nagamachi, S., Ikeda, H., Hamada, M., Chowdhury, V.S., Shinobu Yasuo, Furuse, M.
  • Organizer: 第123回日本畜産学会
• [Book] The Neuroscience of Aging (2020)
  • Author(s): Furuse, M.
  • Publisher: Elsevier
• [Remarks] 研究者情報
  • URL: https://hyoka.ofc.kyushu-u.ac.jp/search/details/K000452/