• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Highly sensitive bio-sniffer devices for body volatile chemicals related to diseases and metabolism

Research Project

Project/Area Number 17H01759
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Perceptual information processing
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Mitsubayashi Kohji  東京医科歯科大学, 生体材料工学研究所, 教授 (40307236)

Co-Investigator(Kenkyū-buntansha) 荒川 貴博  東京医科歯科大学, 生体材料工学研究所, 講師 (50409637)
當麻 浩司  東京医科歯科大学, 生体材料工学研究所, 助教 (40732269)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords皮膚ガス / ガスセンサ / 疾病 / 代謝 / 高感度 / 生体臭成分 / 生化学式ガスセンサ / 薬物代謝酵素 / 光計測 / 疾病・代謝 / 生化学式ガスセ ンサ
Outline of Final Research Achievements

Human volatile components have been reported new biomarker candidates related to some diseases and metabolic conditions. In this project, a fluorometric bio-sniffer device was constructed by incorporating an optical probe into a gas/liquid cell with enzyme diaphragm for detecting a target volatile chemical. Secondary alcohol dehydrogenase (S-ADH) was immobilized into a porous membrane by an enzyme entrapping technique with a biocompatible polymer and applied to bio-sniffer device for a gaseous acetone measurement. The sensor was able to measure acetone vapor (sub-ppb level) with good selectivity based on the specificity of the enzyme. As the physiological application, the sensor also succeeded to be applied for measuring skin acetone vapor involved in lipid metabolism such as aerobic exercise.

Academic Significance and Societal Importance of the Research Achievements

開発したセンサでは、ガス認識素子として酵素を用い、また光学的な検出素子と組み合わせることで、高いガス選択性と高感度なガス計測を実現した。特に、疾病や代謝に基づき呼気や皮膚ガスとして放出される生体ガスの高感度計測に適しており、非侵襲にて血液由来のガス成分を経皮計測を可能とした。また本センサは認識素子である酵素を交換することで、多様なガス成分の計測も可能で今後、早期の疾病スクリーニングや非侵襲での代謝評価などへの応用が考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (24 results)

All 2020 2019 2018 2017

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 9 results,  Invited: 10 results) Book (3 results)

  • [Journal Article] Transcutaneous Blood VOC Imaging System (Skin-Gas Cam) with Real-Time Bio-Fluorometric Device on Rounded Skin Surface2020

    • Author(s)
      Iitani Kenta、Toma Koji、Arakawa Takahiro、Mitsubayashi Kohji
    • Journal Title

      ACS Sensors

      Volume: 5 Issue: 2 Pages: 338-345

    • DOI

      10.1021/acssensors.9b01658

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical and Electrochemical Sensors and Biosensors for the Detection of Quinolones2019

    • Author(s)
      Majdinasab Marjan、Mitsubayashi Kohji、Marty Jean Louis
    • Journal Title

      Trends in Biotechnology

      Volume: 37 Issue: 8 Pages: 898-915

    • DOI

      10.1016/j.tibtech.2019.01.004

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ultrasensitive Sniff-Cam for Biofluorometric-Imaging of Breath Ethanol Caused by Metabolism of Intestinal Flora2019

    • Author(s)
      Iitani Kenta、Toma Koji、Arakawa Takahiro、Mitsubayashi Kohji
    • Journal Title

      Analytical Chemistry

      Volume: 91 Issue: 15 Pages: 9458-9465

    • DOI

      10.1021/acs.analchem.8b05840

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of oral health instructions on glycemic control and oral health status of periodontitis patients with type 2 diabetes mellitus: A preliminary observation2019

    • Author(s)
      Toda Kanako、Mizutani Koji、Minami Isao、Ye Ming、Arakawa Takahiro、Mitsubayashi Kohji、Ogawa Yoshihiro、Araki Kouji、Shinada Kayoko
    • Journal Title

      Journal of Dental Sciences

      Volume: 14 Issue: 2 Pages: 171-177

    • DOI

      10.1016/j.jds.2019.01.009

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Switchable sniff-cam (gas-imaging system) based on redox reactions of alcohol dehydrogenase for ethanol and acetaldehyde in exhaled breath2019

    • Author(s)
      Iitani Kenta、Hayakawa Yuuki、Toma Koji、Arakawa Takahiro、Mitsubayashi Kohji
    • Journal Title

      Talanta

      Volume: 197 Pages: 249-256

    • DOI

      10.1016/j.talanta.2018.12.070

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time monitoring of skin ethanol gas by a high-sensitivity gas phase biosensor (bio-sniffer) for the non-invasive evaluation of volatile blood compounds.2019

    • Author(s)
      Arakawa T, Suzuki T, Tsujii M, Iitani K, Chien PJ, Ye M, Toma K, Iwasaki Y, Mitsubayashi K
    • Journal Title

      Biosens Bioelectron

      Volume: 129 Pages: 245-253

    • DOI

      10.1016/j.bios.2018.09.070

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] バイオセンサ技術を用いた非侵襲生体計測2019

    • Author(s)
      三林浩二
    • Journal Title

      化学工学

      Volume: 83 Pages: 171-174

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Cavitas Biosensors: Noninvasive Approaches to Blood Glucose Monitoring for Diabetes Mellitus2018

    • Author(s)
      Mitsubayashi Kohji
    • Journal Title

      Sensors and Materials

      Volume: 30 Issue: 10 Pages: 2313

    • DOI

      10.18494/SAM.2018.2011

    • ISSN
      0914-4935, 2435-0869
    • Year and Date
      2018-10-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 5.バイオ蛍光式「バイオスニファ」「探嗅カメラ」による高感度な生体ガス計測と可視化応用2018

    • Author(s)
      三林浩二
    • Journal Title

      Denki Kagaku

      Volume: 86 Issue: Summer Pages: 118-122

    • DOI

      10.5796/denkikagaku.18-FE0014

    • NAID

      130007384556

    • ISSN
      2433-3255, 2433-3263
    • Year and Date
      2018-06-05
    • Related Report
      2018 Annual Research Report
  • [Presentation] Bio-fluorometric gas sensing and imaging of human volatiles (Bio-sniffer & Sniff-cam)2020

    • Author(s)
      Kohji Mitsubayashi
    • Organizer
      ICONN 2020
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 気相用バイオセンサとガス濃縮装置を融合したエタノールガス用高感度計測システム2019

    • Author(s)
      石川力、青田 崇志、 當麻 浩司、荒川 貴博、三林 浩二
    • Organizer
      2019SASシンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Gas-phase biosensors (Bio-sniffer and Sniff-cam) for volatile chemicals2019

    • Author(s)
      Kohji Mitsubayashi
    • Organizer
      SPIE 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Gas-phase biosensors for volatile chemicals2019

    • Author(s)
      Kohji Mitsubayashi
    • Organizer
      Huawei Healthcare Workshop
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Principle of Gas phase biosensors: Biosniffer & Sniff-cam for medical and healthcare applications2019

    • Author(s)
      Kohji Mitsubayashi, Takahiro Arakawa
    • Organizer
      ISOEN 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Gas-Phase Biosensors (Bio-Sniffer & Sniff-Cam) for Human Volatile Chemicals2019

    • Author(s)
      Kohji Mitsubayashi
    • Organizer
      ICBME2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Non-invasive Biosensing Devices for Preemptive Medicine2019

    • Author(s)
      Kohji Mitsubayashi
    • Organizer
      Bio4Apps2018/2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 疾病の超早期診断のための呼気・皮膚ガス成分の高感度バイオ計測&イメージング2018

    • Author(s)
      三林浩二
    • Organizer
      CSJ化学フェスタ(日本化学会)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Non-invasive biomedical devices for the future of medicine2018

    • Author(s)
      Kohji Mitsubayashi
    • Organizer
      SDM-18 (Sustainable Design and Manufacturing 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cavitas biosensors for IoT health care & preventive medicine2018

    • Author(s)
      Kohji Mitsubayashi
    • Organizer
      A Joint Taiwan-Japan Research Grant 2018, “Secure IoT-Based Information Platform with Privacy-Preserving Data Mining on Big Data for M-Healthcare”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sniff-cam for real-time imaging of volatile chemicals2018

    • Author(s)
      Kohji Mitsubayashi
    • Organizer
      DOS annual meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 二級アルコール脱水素酵素を用いたアセトン用バイオセンサ2017

    • Author(s)
      青田 崇志、簡 伯任、叶 明、當麻 浩司、荒川 貴博、三林 浩二
    • Organizer
      '17 SAS Symposium
    • Related Report
      2017 Annual Research Report
  • [Book] 酵素トランスデューサーと酵素技術展開2020

    • Author(s)
      三林浩二
    • Total Pages
      300
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314884
    • Related Report
      2019 Annual Research Report
  • [Book] Chemical, Gas, and Biosensos for Internet of Things and Related Applications2019

    • Author(s)
      Kohji MITSUBAYASHI, Osamu NIWA, Yuko UENO
    • Total Pages
      406
    • Publisher
      Elsevier
    • Related Report
      2019 Annual Research Report
  • [Book] 代謝センシング -健康、食、美容、薬、そして脳の代謝を知る-2018

    • Author(s)
      三林浩二
    • Total Pages
      249
    • Publisher
      (株)シーエムシー出版
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi