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Production of Daphnia for endocrine disruptor sensing

Research Project

Project/Area Number 17H01880
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation and chemicals
Research InstitutionOsaka University

Principal Investigator

Watanabe Hajime  大阪大学, 工学研究科, 教授 (80212322)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywords化学物質影響 / ミジンコ / ゲノム編集 / 核内受容体 / 内分泌かく乱化学物質 / 蛍光タンパク質 / ホルモン / バイオアッセイ / 遺伝子編集 / レポーター遺伝子 / モニタリング / エストロゲン / 生態影響 / レポーター / Gal4 / CRISPR/Cas9 / Gal4
Outline of Final Research Achievements

In order to evaluate the biological effects of chemicals, it is important to develop methods using living organisms in addition to the conventional analytical approaches. Chemicals have a very wide range of mod of actions, one of which is hormone-like chemicals. In this study, we aimed to introduce a human-type chemical response system based on nuclear receptors into Daphnia magna. We improved the genome editing efficiency by modifying the established transgene transfer system, and established a system that efficiently transfers foreign genes without position effects. Using this system, we succeeded to introduce a human-type chemical response system. We confirmed the gene response of this human-type transgenic Daphnia magna by actual exposure of hormone like chemicals.

Academic Significance and Societal Importance of the Research Achievements

従来はヒトにおける化学物質影響を評価するためには培養細胞を用いた解析が必要であり、コストと時間がかかっていた。本研究では、一連の関連遺伝子をミジンコに導入することに成功し、実際に化学物質やホルモンによって導入された遺伝子が応答するこを確認した。これは従来の培養細胞を用いた手法とことなり、飼育が用意で安価に維持できるミジンコを用いてヒトへの化学物質影響評価が可能になることを示している。本研究を展開することにより、安価で簡便に化学物質影響を評価することが可能になることが期待できる。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (85 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (25 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 24 results,  Open Access: 18 results) Presentation (54 results) (of which Int'l Joint Research: 7 results,  Invited: 7 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] Birmingham University(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Katholieke Universiteit Leuven(ベルギー)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Mutation of the Cytochrome P450 CYP360A8 Gene Increases Sensitivity to Paraquat in Daphnia magna2021

    • Author(s)
      Religia Pijar、Nguyen Nhan Duc、Nong Quang Dang、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      Environmental Toxicology and Chemistry

      Volume: 0 Issue: 5 Pages: 0-0

    • DOI

      10.1002/etc.4970

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression2021

    • Author(s)
      Perez Christelle Alexa Garcia、Adachi Shungo、Nong Quang Dang、Adhitama Nikko、Matsuura Tomoaki、Natsume Toru、Wada Tadashi、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      PLOS Genetics

      Volume: 17 Issue: 7 Pages: e1009683-e1009683

    • DOI

      10.1371/journal.pgen.1009683

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNMT3.1 controls trade-offs between growth, reproduction, and life span under starved conditions in Daphnia magna2021

    • Author(s)
      Nguyen Nhan Duc、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 0-0

    • DOI

      10.1038/s41598-021-86578-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oligosaccharides derived from dragon fruit modulate gut microbiota, reduce oxidative stress and stimulate toll-pathway related gene expression in freshwater crustacean Daphnia magna2020

    • Author(s)
      Sangkuanun Thanwarat、Wichienchot Santad、Kato Yasuhiko、Watanabe Hajime、Peerakietkhajorn Saranya
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 103 Pages: 126-134

    • DOI

      10.1016/j.fsi.2020.04.046

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Two Doublesex1 mutants revealed a tunable gene network underlying intersexuality in Daphnia magna2020

    • Author(s)
      Nong Quang Dang、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 8 Pages: e0238256-e0238256

    • DOI

      10.1371/journal.pone.0238256

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna2020

    • Author(s)
      Adhitama Nikko、Kato Yasuhiko、Matsuura Tomoaki、Watanabe Hajime
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 10 Pages: e0239893-e0239893

    • DOI

      10.1371/journal.pone.0239893

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Caloric restriction upregulates the expression ofDNMT3.1, lacking the conserved catalytic domain, inDaphnia magna2020

    • Author(s)
      Nguyen Nhan Duc、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      genesis

      Volume: 58 Issue: 12

    • DOI

      10.1002/dvg.23396

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of genome-edited Daphnia for heavy metal detection by fluorescence2020

    • Author(s)
      Arao Takuto、Kato Yasuhiko、Nong Quang Dang、Yamamoto Hiroshi、Watanabe Haruna、Matsuura Tomoaki、Tatarazako Norihisa、Tani Kazune、Okamoto Akira、Matsumoto Takeru、Watanabe Hajime
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 21490-21490

    • DOI

      10.1038/s41598-020-78572-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reduction of histamine and enhanced spinning behavior of Daphnia magna caused by scarlet mutant2020

    • Author(s)
      Ismail Nur Izzatur Binti、Kato Yasuhiko、Matsuura Tomoaki、G?mez‐Canela Cristian、Barata Carlos、Watanabe Hajime
    • Journal Title

      genesis

      Volume: 59 Issue: 3

    • DOI

      10.1002/dvg.23403

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Variations in effects of ectosymbiotic microbes on the growth rates among different species and genotypes of Daphnia fed different algal diets2020

    • Author(s)
      Zhang Xuan、Ohtsuki Hajime、Makino Wataru、Kato Yasuhiko、Watanabe Hajime、Urabe Jotaro
    • Journal Title

      Ecological Research

      Volume: 36 Issue: 2 Pages: 303-312

    • DOI

      10.1111/1440-1703.12194

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vitro synthesis of the human calcium transporter Letm1 within cell-sized liposomes and investigation of its lipid dependency2019

    • Author(s)
      Okamura Kosuke、Matsushita Shuhei、Kato Yasuhiko、Watanabe Hajime、Matsui Aiko、Oka Toshihiko、Matsuura Tomoaki
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 127 Issue: 5 Pages: 544-548

    • DOI

      10.1016/j.jbiosc.2018.11.003

    • NAID

      40021891579

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Parasite‐mediated selection in a natural metapopulation of Daphnia magna2019

    • Author(s)
      Cabalzar Andrea P.、Fields Peter D.、Kato Yasuhiko、Watanabe Hajime、Ebert Dieter
    • Journal Title

      Molecular Ecology

      Volume: 28 Issue: 21 Pages: 4770-4785

    • DOI

      10.1111/mec.15260

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Class?III Polyphosphate Kinase?2 Enzymes Catalyze the Pyrophosphorylation of Adenosine‐5′‐Monophosphate2019

    • Author(s)
      Ogawa Marin、Uyeda Atsuko、Harada Kazuo、Sato Yu、Kato Yasuhiko、Watanabe Hajime、Honda Kohsuke、Matsuura Tomoaki
    • Journal Title

      ChemBioChem

      Volume: 20 Issue: 23 Pages: 2961-2967

    • DOI

      10.1002/cbic.201900303

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 甲殻類ミジンコの性決定2019

    • Author(s)
      加藤泰彦、渡邉肇
    • Journal Title

      昆虫と科学

      Volume: 1 Pages: 4-7

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna2019

    • Author(s)
      Religia Pijar、Kato Yasuhiko、Fukushima Ery Odette、Matsuura Tomoaki、Muranaka Toshiya、Watanabe Hajime
    • Journal Title

      Marine Environmental Research

      Volume: 145 Pages: 177-183

    • DOI

      10.1016/j.marenvres.2019.02.007

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tryptophan hydroxylase (TRH) loss of function mutations induce growth and behavioral defects in Daphnia magna2018

    • Author(s)
      Rivetti Claudia、Campos Bruno、Pina Benjamin、Raldua Demetrio、Kato Yasuhiko、Watanabe Hajime、Barata Carlos
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1518-1518

    • DOI

      10.1038/s41598-018-19778-0

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microbiota inoculum composition affects holobiont assembly and host growth in Daphnia2018

    • Author(s)
      Martijn Callens、Hajime Watanabe、Yasuhiko Kato、Jun Miura、Ellen Decaestecker
    • Journal Title

      Microbiome

      Volume: 6 Issue: 1 Pages: 56-56

    • DOI

      10.1186/s40168-018-0444-1

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna2018

    • Author(s)
      Toerner Kerstin、Nakanishi Tsuyoshi、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 6 Pages: e0198023-e0198023

    • DOI

      10.1371/journal.pone.0198023

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Generation of white-eyed Daphnia magna mutants lacking scarlet function2018

    • Author(s)
      Ismail Nur Izzatur Binti、Kato Yasuhiko、Matsuura Tomoaki、Watanabe Hajime
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 11 Pages: e0205609-e0205609

    • DOI

      10.1371/journal.pone.0205609

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A 5′ UTR-Overlapping LncRNA Activates the Male-Determining Gene doublesex1 in the Crustacean Daphnia magna2018

    • Author(s)
      Kato Yasuhiko、Perez Christelle Alexa G.、Mohamad Ishak Nur Syafiqah、Nong Quang D.、Sudo Yuumi、Matsuura Tomoaki、Wada Tadashi、Watanabe Hajime
    • Journal Title

      Current Biology

      Volume: 28 Issue: 11 Pages: 1811-1817

    • DOI

      10.1016/j.cub.2018.04.029

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monitoring ecdysteroid activities using genetically encoded reporter gene in Daphnia magna2018

    • Author(s)
      Adhitama Nikko、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      Marine Environmental Research

      Volume: 140 Pages: 375-381

    • DOI

      10.1016/j.marenvres.2018.07.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A murine model of atopic dermatitis can be generated by painting the dorsal skin with hapten twice 14 days apart2018

    • Author(s)
      Kitamura Ayaka、Takata Ryohei、Aizawa Shin、Watanabe Hajime、Wada Tadashi
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 5988-5988

    • DOI

      10.1038/s41598-018-24363-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter2017

    • Author(s)
      Nong Quang Dang、Mohamad Ishak Nur Syafiqah、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 13521-13521

    • DOI

      10.1038/s41598-017-13730-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of a bicistronic expression system in the branchiopod crustacean Daphnia magna2017

    • Author(s)
      Kumagai Hitoshi、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      genesis

      Volume: 55 Issue: 12

    • DOI

      10.1002/dvg.23083

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna2017

    • Author(s)
      Kumagai Hitoshi、Nakanishi Takashi、Matsuura Tomoaki、Kato Yasuhiko、Watanabe Hajime
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 10 Pages: e0186112-e0186112

    • DOI

      10.1371/journal.pone.0186112

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Functional analysis of the lncRNA interacting protein CELF2 in regulating the sex-determining gene dsx1 in Daphnia magna2021

    • Author(s)
      Tirta, Y. K., Adachi, S., Perez, C.A.G., Nong, Q.D., Natsume, T., Kato, Y., Watanabe, H.
    • Organizer
      第22回日本RNA学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Sense-overlapping lncRNA as a decoy of translational repressor protein for dimorphic gene expression2021

    • Author(s)
      Perez, C.A.G., Adachi, S., Nong, Q.D., Adhitama, N., Natsume, T., Wada, T., Kato, Y., Watanabe, H.
    • Organizer
      第22回日本RNA学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 遺伝子編集ミジンコを用いた環境水中のホルモン様活性のバイオモニタリング2021

    • Author(s)
      Hsieh, L-C., 野田彩乃、Nikko Adhitama、加藤泰彦、渡邉肇
    • Organizer
      第48回日本毒性学会学術年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 環境水のホルモン様活性可視化ミジンコの作製2020

    • Author(s)
      加藤泰彦、野田彩乃、Nikko Adhitama、渡邉肇
    • Organizer
      第47回日本毒性学会学術年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Functional analyses of Polycomb in Daphnia magna2020

    • Author(s)
      Leim. J., Kato. Y., Watanabe, H.
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] オオミジンコ (Daphnia magna) の脂肪酸代謝における共生菌の機能解析2020

    • Author(s)
      丸山樹生、加藤泰彦、渡邉肇
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] オオミジンコにおける生存戦略を決定する化学コミュニケーションの解明2020

    • Author(s)
      西上旺希、加藤泰彦、渡邉肇
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] オオミジンコへのオーキシンデグロンシステムの導入2020

    • Author(s)
      阪本寛菜、西村浩平、加藤泰彦、渡邉肇
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] DNMT3.1は、ミジンコ類の甲殻類Daphnia magnaの飢餓状態での寿命と、成長と繁殖力の間のトレードオフを制御します。2020

    • Author(s)
      Nguyen. N.、松浦友亮、加藤泰彦、渡邉肇
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 甲殻類ミジンコにおけるメラニン生合成遺伝子の機能解析2020

    • Author(s)
      林笑梨紗、加藤泰彦、渡邉肇
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] オオミジンコにおける非相同末端結合に関わる遺伝子のノックアウト2020

    • Author(s)
      宮地秀幸、Fatimah, R.M., 中西貴士、加藤泰彦、渡邉肇
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Roles of and interaction between ecdysteroid and sesquiterpenoid biosynthesis genes in embryogenesis of Daphnia magna2020

    • Author(s)
      Adhitama. N., Kato. Y., Matsuura, T., Watanabe, H
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Development of transgenic Daphnia magna for visualizing homology directed repair of DNA double strand break2020

    • Author(s)
      Fatimah. R. M., Kato. Y., Matsuura, T., Watanabe, H.
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] GAL4/UASシステムを用いたオオミジンコのエクシステロイド活性検出への応用2020

    • Author(s)
      山本雄大、山_勇人、加藤泰彦、渡邉肇
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular mechanism of adaptive sex ratio adjustment in the water flea Daphnia magna2020

    • Author(s)
      Yasuhiko Kato, Quang D. Nong, Christelle Alexa G. Pelez, Nur Syafiqah Mohamad Ishak, Hajime Watanabe
    • Organizer
      EMBL Symposia "The Organism and its Environment"
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanism of adaptive sex ratio adjustment in the water flea Daphnia magna2020

    • Author(s)
      Yasuhiko Kato, Quang Dang Nong, Christelle Alexa Garcia Perez, Nur Syafiqah Mohamad Ishak, Hajime Watanabe
    • Organizer
      EMBO | EMBL Symposia The Organism and its Environment
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Monitoring of hormonal activities in the water flea Daphnia magna using genome editing tools2020

    • Author(s)
      Yasuhiko Kato, Ayano Noda, Yudai Yamamoto, Yuto Yamazaki, Nikko Adhitama, Hajime Watanabe
    • Organizer
      EMBO | EMBL Symposia The Organism and its Environment
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生殖戦略を決定する化学コミュニケーションの解明2019

    • Author(s)
      西上旺希、加藤泰彦、渡邉肇
    • Organizer
      新学術領域研究「化学コミュニケーションのフロンティア」第5回公開シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] オオミジンコにおける長鎖ノンコーディングRNAによるdoublesex1遺伝子の活性化2019

    • Author(s)
      加藤 泰彦,Perez Christelle Alexa,Nong Quang,Mohamad Ishak Nur Syafiqah,和田 忠志,渡邉 肇
    • Organizer
      日本動物学会 第90回 大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna.2019

    • Author(s)
      Pijar Religia, Yasuhiko Kato, Ery Odette Fukushima, Tomoaki Matsuura, Toshiya Muranaka, Hajime Watanabe
    • Organizer
      日本動物学会 第90回 大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 環境応答により雌雄を産み分けるミジンコの性スペクトラム2019

    • Author(s)
      加藤 泰彦,Perez Christelle Alexa,Nong Quang,渡邉 肇
    • Organizer
      新学術領域研究「性スペクトラム _連続する表現型としての雌雄」第3回領域会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 遺伝子組み換え技術を応用したオオミジンコにおける幼若ホルモン活性の可視化2019

    • Author(s)
      野田彩乃、加藤泰彦、松浦友亮、渡邉肇
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ビテロジェニン輸送経路を利用した組換えタンパク質のミジンコ卵への蓄積系2019

    • Author(s)
      辻 勇祐, 加藤 泰彦, 渡邉 肇
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Development of a reporter system to visualize juvenile hormone activity in Daphnia magna2019

    • Author(s)
      野田彩乃、加藤泰彦、松浦友亮、渡邉肇
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ミジンコにおける生存戦略を決定する化学コミュニケーションの解明2019

    • Author(s)
      西上旺希、加藤泰彦、渡邉肇
    • Organizer
      「化学コミュニケーションのフロンティア」 第 6 回公開シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ミジンコにおけるオス決定因子Dsx1発現細胞のトランスクリプトーム解析2019

    • Author(s)
      加藤泰彦, Quang Dang Nong, 渡邉肇
    • Organizer
      「先進ゲノム支援」2019年度拡大班会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] ミジンコにおける性特異的な長鎖ノンコーディングRNAの探索2019

    • Author(s)
      加藤泰彦, Christelle Alexa Gracia Perez, 渡邉肇
    • Organizer
      「先進ゲノム支援」2019年度拡大班会議
    • Related Report
      2019 Annual Research Report
  • [Presentation] 生殖戦略を決定する化学コミュニケーションの解明2018

    • Author(s)
      渡邉肇
    • Organizer
      新学術領域研究「化学コミュニケーションのフロンティア」第3回公開シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] A 5´ UTR-overlapping LncRNA Activates the Male-determining Gene doublesex1 in the Crustacean Daphnia magna2018

    • Author(s)
      Yasuhiko Kato, Christelle Alexa G. Pelez, Nur Syafiqah Mohamad Ishak, Quang D. Nong, Tomoaki Matsuura, Tadashi Wada, Hajime Watanabe
    • Organizer
      第 20 回 日本 RNA 学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] オオミジンコにおける毒性影響可視化のための遺伝子工学的手法の開発2018

    • Author(s)
      渡邉肇
    • Organizer
      第45回日本毒性学会学術年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Monitoring of chemical and metal contaminants using engineered Daphnia magna2018

    • Author(s)
      Yasuhiko Kato, Hajime Watanabe
    • Organizer
      Second Interdisciplinary and Research Alumni Symposium iJaDe2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オオミジンコにおける内分泌かく乱物質検出のためのGAL4/UASシステムの確立2018

    • Author(s)
      加藤泰彦、山崎勇人、松浦友亮、渡邉肇
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Visualization of ecdysteroid activities using genetically encoded reporter gene in Daphnia magna2018

    • Author(s)
      Nikko Adhitama, Yasuhiko Kato, Tomoaki Matsuura, Hajime Watanabe
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 気液界面培養下の気道上皮細胞への排ガス曝露影響評価法の検討-(1)送気流量の検討-2018

    • Author(s)
      伊藤剛、村木直美、田村久美子、利根川義男、石井幸雄、酒井康行、渡邉肇、高野裕久
    • Organizer
      第59回大気環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 気液界面培養下の気道上皮細胞への排ガス曝露影響評価法の検討-(2)線維芽細胞共培養の有効性の検討-2018

    • Author(s)
      村木直美、伊藤剛、田村久美子、利根川義男、石井幸雄、酒井康行、渡邉肇、高野裕久
    • Organizer
      第59回大気環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 再構成型無細胞翻訳系を用いたGタンパク質共役型受容体の安定化変異体の取得2018

    • Author(s)
      仲井宏紀、一色衣香、渡邉肇、村田武士、松浦友亮
    • Organizer
      「細胞を創る」研究会11.0
    • Related Report
      2018 Annual Research Report
  • [Presentation] In vitro evolution of E. coli multidrug efflux transporter EmrE by using liposome display2018

    • Author(s)
      内田紗衣、植田淳子、渡邊肇、松浦友亮
    • Organizer
      「細胞を創る」研究会11.0
    • Related Report
      2018 Annual Research Report
  • [Presentation] オオミジンコの遺伝子操作法の開発と応用2018

    • Author(s)
      加藤泰彦、志賀靖弘、時下進一、山形秀夫、渡邉肇
    • Organizer
      東京薬科大学生命科学部25周年記念シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 環境応答により雌雄を産み分けるミジンコの性スペクトラム2018

    • Author(s)
      Yasuhiko Kato、Christelle Alexa G. Perez、Quang D. Nong、Hajime Watanabe
    • Organizer
      新学術領域研究 性スペクトラム 第二回 領域班会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] 多剤排出トランスポーターEmrEを検出素子とした細胞毒性化合物検出技術の開発2018

    • Author(s)
      服部将人, 渡邉肇, 松浦友亮
    • Organizer
      第13回無細胞生命科学研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A 5' UTR-Overlapping LncRNA Activates the Male- Determining Gene doublesex1 in the Crustacean Daphnia magna2018

    • Author(s)
      Yasuhiko Kato、Christelle Alexa G. Perez、Quang D. Nong、Hajime Watanabe
    • Organizer
      JAJ RNA 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] オオミジンコにおけるHSP70プロモーターを利用した熱誘導型遺伝子発現系の開発2018

    • Author(s)
      加藤泰彦、清水里奈、渡邉肇
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 環境応答により雌雄を産み分けるミジンコの性スペクトラム2018

    • Author(s)
      加藤泰彦、Quang Dang Nong、渡邉肇
    • Organizer
      「先進ゲノム支援」2018年度拡大班会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] Alterations in the activity of the sex determination factor dsx1 resulted in different intersex phenotypes in Daphnia magna2018

    • Author(s)
      Quang Dang Nong , Yasuhiko Kato , Hajime Watanabe
    • Organizer
      「先進ゲノム支援」2018年度拡大班会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] Possibility of chemical communication among Daphnia individuals for reproductive strategy2018

    • Author(s)
      Yasuhiko Kato, Hajime Watanabe
    • Organizer
      Frontier Research on Chemical Communications (ISCC2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生態学と分子生物学の接点としてのミジンコ2018

    • Author(s)
      渡邉肇
    • Organizer
      日本生態学会 第66回大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] オオミジンコの周期性単為生殖における環境依存的な性決定メカニスズム2017

    • Author(s)
      加藤泰彦、Quang Dang Nong、Nur Syafiqah Mohamad Ishak、渡邉肇
    • Organizer
      日本動物学会第88回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ミジンコの環境依存的な性決定を制御する長鎖非コードRNA2017

    • Author(s)
      加藤泰彦、Christelle Alexa Pelez、Nong Dang Quang、渡邉肇
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] オオミジンコにおけるCas9タンパク質を用いた高効率な遺伝子破壊法と遺伝子導入法の開発2017

    • Author(s)
      熊谷仁志、中西貴士、松浦友亮、加藤泰彦、渡邉肇
    • Organizer
      第2回ゲノム編集学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] オオミジンコにおける眼の色素遺伝子 scarletの遺伝子組み換えマーカーとしての検討2017

    • Author(s)
      Nur Izzatur Binti Ismail、加藤泰彦、松浦友亮、渡邉肇
    • Organizer
      第2回ゲノム編集学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] TALEN を利用した非相同末端結合によるオオミジンコゲノムへのエストロゲンバイオセンサーの導入2017

    • Author(s)
      Torner Kerstin、加藤泰彦、渡邉肇
    • Organizer
      第2回ゲノム編集学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 重金属曝露を可視化したミジンコの作製2017

    • Author(s)
      荒尾拓斗、加藤泰彦、渡邉肇
    • Organizer
      第44回日本毒性学会学術年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲノム編集技術によるミジンコの性決定機構の解析2017

    • Author(s)
      加藤泰彦、渡邉肇
    • Organizer
      日本動物学会 第88回 富山大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Genome editing for visualization of water pollutants using water flea2017

    • Author(s)
      Yasuhiko Kato, Hajime Watanabe
    • Organizer
      19th International Symposium on Pollutant Responses in Marine Organisms
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] The Chemical Biology of Long Noncoding RNAs2020

    • Author(s)
      Stefan JurgaJan Barciszewski (Yasuhiko Kato, Hajime Watanabe)
    • Total Pages
      521
    • Publisher
      Springer Nature
    • ISBN
      9783030447427
    • Related Report
      2020 Annual Research Report
  • [Remarks] 大阪大学工学研究科生命先端工学専攻渡邉研究室

    • URL

      http://www.bio.eng.osaka-u.ac.jp/ez/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 大阪大学工学研究科生命先端工学専攻 渡邉研究室

    • URL

      http://www.bio.eng.osaka-u.ac.jp/ez/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 生命環境システム工学

    • URL

      http://www.bio.eng.osaka-u.ac.jp/ez/index.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

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