• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of basic metabolism of protozoa responsible for sewage treatment process and elucidation of microbial metabolism network

Research Project

Project/Area Number 17H01901
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Modeling and technologies for environmental conservation and remediation
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Shinzato Naoya  琉球大学, 熱帯生物圏研究センター, 准教授 (00381252)

Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords嫌気環境 / 原生動物 / 細胞内共生 / ゲノム解読 / 脂肪酸合成 / 嫌気 / メタン / バクテリア / 排水処理 / 水処理技術 / メタン生成 / ゲノム
Outline of Final Research Achievements

In the present study, we analyzed in detail the whole genome information of the bacterial symbiont TC1 living in Trimyema ciliate, which plays a major role in a sewage treatment process. As the results, it was shown that TC1 may have obtained amino acids from host ciliate and actively synthesized lipids. In addition, it was revealed that another ciliate closely related to the genus Cyclidium also have symbiotic bacteria of unknown function in addition to methanogens, which was named Hydrogenosomobacter endosymbioticus. Histochemical analysis showed that both methanogens and Hydrogenosomobacter symbiont are in close contact with hydrogen-generating organelle, hydrogenosomes.

Academic Significance and Societal Importance of the Research Achievements

下水処理プロセスで水の浄化に主要な役割を果たす原生動物は、メタン生成菌を始めとした原核微生物を細胞内に共生させていることが多い。メタン生成菌は、宿主原生動物の嫌気代謝の結果生じる水素をメタン化することで、宿主による不溶性有機物の代謝を活性化している。その一方で、それ以外の共生微生物については宿主への寄与が明らかとなっていなかった。本研究は、下水処理プロセスで働く原生動物の共生バクテリアが、脂質合成を行って宿主へ供給するとともに脂質顆粒の形成を促し、宿主の飢餓条件への適応度を高めている可能性を示した。本研究成果は、原生動物の代謝基盤の理解を深め、プロセスの安定化や高度化に寄与すると考えられる。

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (5 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Tripartite Symbiosis of an Anaerobic Scuticociliate with Two Hydrogenosome-Associated Endosymbionts, a Holospora-Related Alphaproteobacterium and a Methanogenic Archaeon2019

    • Author(s)
      Takeshita Kazutaka、Yamada Takanori、Kawahara Yuto、Narihiro Takashi、Ito Michihiro、Kamagata Yoichi、Shinzato Naoya
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 85 Issue: 24 Pages: 24-24

    • DOI

      10.1128/aem.00854-19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 嫌気性繊毛虫GW7株におけるメタン生成アーキア、バクテリアとの共生関係2019

    • Author(s)
      竹下和貴, 山田尊貴, 川原邑斗, 成廣 隆, 伊藤通浩, 鎌形洋一, 山崎 玲, 新里 尚也
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Endosymbiotic interaction in anaerobic ciliates with methanogens and bacteria2018

    • Author(s)
      Kazutaka Takeshita, Takanori Yamada, Yuto Kawahara, Takashi Narihiro, Yoichi Kamagata, Naoya Shinzato
    • Organizer
      32nd Annual Meeting of Japanese Society for Microbial Ecology & 10th Asian Symposium on Microbial Ecology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 培養に成功した嫌気性繊毛虫シクリディウム原虫における3者間共生2017

    • Author(s)
      竹下和貴、山田尊貴、成廣隆、鎌形洋一、新里尚也
    • Organizer
      環境微生物系合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Book] (Endo)symbiotic Methanogenic Archaea (Microbiology Monographs Book 19) 2nd Edition2018

    • Author(s)
      Naoya Shinzato, Kazutaka Takeshita, Yoichi Kamagata
    • Total Pages
      261
    • Publisher
      Springer
    • ISBN
      9783319988351
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi