Development of in vitro and in vivo test methods for evaluation of environmental pharmaceuticals on the aquatic species
| Project/Area Number |
17H01907
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental risk control and evaluation
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| Research Institution | Kyoto University |
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Principal Investigator |
Ihara Masaru 京都大学, 工学研究科, 特定助教 (70450202)
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| Co-Investigator(Kenkyū-buntansha) |
長江 真樹 長崎大学, 水産・環境科学総合研究科(環境), 教授 (00315227)
田中 宏明 京都大学, 工学研究科, 教授 (70344017)
征矢野 清 長崎大学, 海洋未来イノベーション機構, 教授 (80260735)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Keywords | GPCR阻害薬 / 抗うつ薬 / in vitroアッセイ / in vivo曝露試験 / 行動異常 / 下水 / 河川水 / 魚 / セロトニントランスポーター / 魚曝露試験 / 環境医薬品 / 魚類 / 行動影響 / 培養細胞試験 / 薬理活性 / 医薬品生理活性 / 細胞培養試験 / 行動試験 / ＧＰＣＲ作用薬 / 魚行動試験 / ジフェンヒドラミン / 環境 / 生物影響評価 / 行動解析 / 下水道 |
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| Outline of Final Research Achievements |
Pharmaceuticals taken by human flow into the sewage treatment plant, and after sewage treatment process, are released into receiving waters such as rivers. Since many kinds of pharmaceuticals act on nerve cells, it is essential to evaluate from the viewpoint of behavioral abnormalities when fish are exposed to them in river. The purpose of this study was to establish the evaluation methods and to clarify the degree of pharmaceutical contamination of river water and sewage. Specifically, we comprehensively measured and clarified the pharmacological activities of GPCR inhibitors and antidepressants against human and fish receptors by in vitro tests. Furthermore, the degree of pharmacological activity in sewage treatment plants in Japan and the United Kingdom was clarified. In addition, we were able to establish a in vivo testing system that can track the behavior of fish with a video camera and establish the basis for analysis of behavioral abnormalities.
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| Academic Significance and Societal Importance of the Research Achievements |
GPCR阻害薬と抗うつ薬の活性を測定できるin vitro試験系を世界で初めて下水へ適用し、その実態を明らかにした。さらにヒトの受容体と魚の受容体の医薬品に対する感受性の違いも世界で初めてデータを示すことができた。生物毒性研究においてこれまで体系的に調べられてこなかった神経系医薬品の研究の必要性を示す重要な成果である。また、これまで報告されている医薬品曝露による水生生物の行動異常の作用機序の説明につながる重要な成果である。
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Report
(4 results)
Research Products
(40 results)
