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Determination of dependence of Japanese macaques on farmland using gut micro bacteria and establishment of a method to judge the level of damage

Research Project

Project/Area Number 17H01911
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental and ecological symbiosis
Research InstitutionKobe University

Principal Investigator

Kiyono Mieko  神戸大学, 人間発達環境学研究科, 准教授 (40570966)

Co-Investigator(Kenkyū-buntansha) 山端 直人  兵庫県立大学, 自然・環境科学研究所, 教授 (00503856)
早川 卓志  北海道大学, 地球環境科学研究院, 助教 (00758493)
森光 由樹  兵庫県立大学, 自然・環境科学研究所, 准教授 (20453160)
半谷 吾郎  京都大学, 霊長類研究所, 准教授 (40444492)
福田 伊津子  神戸大学, 農学研究科, 助教 (50418943)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywordsニホンザル / 加害レベル / 保全管理 / 腸内細菌 / 野生動物管理 / 腸内細菌叢 / 自然共生システム / 生態系保全・管理 / 生態系管理・保全 / 獣害 / 悪質化 / 獣害対策
Outline of Final Research Achievements

The Japanese macaque is endemic to Japan and has been recognized as harmful animals. In this study, we investigated the effects of the anthropogenic environment on the gut microbiota. The results showed that the intestinal microflora of the crop-raiding and captive troops differed from that of the natural group. Further studies revealed that the differences in the intestinal microflora were not only related to the anthropogenic environment but also to regional differences. In this study, we examined the possibility of using the gut microbiota as an index for determining the level of aggression for conservation management of Japanese macaques. Although the gut microbiota is effective in determining the level of damage, it also suggests that even crop-raiding troops have characteristics similar to those of natural groups. The gut microbiota is useful as a method to reconsider the Japanese macaque population as a gradation that does not depend on whether it is a crop-raiding troop or not.

Academic Significance and Societal Importance of the Research Achievements

ニホンザル等の哺乳類は生物多様性保全において重要な種として位置付けられるだけでなく、国際的にも貴重な種である。こうした種が人間との軋轢によって絶滅へと転じる問題は世界的にも散見される。従って適切な保全管理が重要であり、ニホンザルの場合は群ごとに加害レベルを判定し、レベルに応じて対応する方針になっている。しかしながら、その加害レベル判定は人間活動との接点が基準であり、ニホンザルの生態学的な特徴を加味したものとは言い難い。本研究は、飼育群・獣害群・自然群といった人為的環境との接点の違いを評価する腸内細菌叢の有用性を検討したものである。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (26 results)

All 2021 2020 2019 2018 2017

All Journal Article (15 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 14 results,  Open Access: 3 results) Presentation (11 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Stomach and colonic microbiome of wild Japanese macaques2021

    • Author(s)
      Lee W, Hayakawa T, Kurihara Y, Hanzawa M, Sawada A, Kaneko A, Morimitsu Y, Natsume T, Aisu S, Ito T, Honda T, Hanya G
    • Journal Title

      Am J Primatol

      Volume: 83 Issue: 5

    • DOI

      10.1002/ajp.23242

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fermentation Ability of Gut Microbiota of Wild Japanese Macaques in the Highland and Lowland Yakushima: In Vitro Fermentation Assay and Genetic Analyses2020

    • Author(s)
      Hanya Goro、Tackmann Janko、Sawada Akiko、Lee Wanyi、Pokharel Sanjeeta Sharma、de Castro Maciel Valdevino Gisele、Toge Akito、Kuroki Kota、Otsuka Ryoma、Mabuchi Ryoma、Liu Jie、Hatakeyama Masaomi、Yamasaki Eri、von Mering Christian、Shimizu-Inatsugi Rie、Hayakawa Takashi、Shimizu Kentaro K.、Ushida Kazunari
    • Journal Title

      Microbial Ecology

      Volume: 80 Issue: 2 Pages: 459-474

    • DOI

      10.1007/s00248-020-01515-8

    • NAID

      120006876578

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Island-wide Distribution of Japanese Macaques in Yakushima Revealed by Census Targeting Feces on Roads in 2017-20182020

    • Author(s)
      半谷吾郎、好廣眞一、YANG Danhe、WONG Christopher Chai Thiam、岡桃子、楊木萌、佐藤侑太郎、大坪卓、櫻井貴之、川田美風、F. FAHRI、SIWAN Elangkumaran Sagtia、HAVERCAMP Kristin、余田修助、GU Ningxin、LOKHANDWALA Seema Sheesh、中野勝光、瀧雄渡、七五三木環、本郷峻、澤田晶子、本田剛章、栗原洋介
    • Journal Title

      Primate Research

      Volume: 36 Issue: 2 Pages: 23-31

    • DOI

      10.2354/psj.36.014

    • NAID

      130007959149

    • ISSN
      0912-4047, 1880-2117
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gut microbiota composition of Japanese macaques associates with extent of human encroachment2019

    • Author(s)
      Lee Wanyi、Hayakawa Takashi、Kiyono Mieko、Yamabata Naoto、Hanya Goro
    • Journal Title

      American Journal of Primatology

      Volume: - Issue: 12

    • DOI

      10.1002/ajp.23072

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 柿の利活用を通じた多様な主体による獣害対策の可能性ー兵庫県丹波篠山市の事例からー2019

    • Author(s)
      清野未恵子
    • Journal Title

      Wildlife Forum

      Volume: 24 Pages: 3-6

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Within-Population Variations in Home Range Use and Food Patch Use of Japanese Macaques: A Perspective of Intergroup Hostility2018

    • Author(s)
      Kurihara Yosuke、Hanya Goro
    • Journal Title

      Folia Primatologica

      Volume: 89 Issue: 6 Pages: 397-414

    • DOI

      10.1159/000493574

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal variation of energy expenditure in Japanese macaques (Macaca fuscata)2018

    • Author(s)
      Sha John Chih Mun、Kurihara Yosuke、Tsuji Yamato、Take Makiko、He Tianmeng、Kaneko Akihisa、Suda-Hashimoto Naoko、Morimoto Mayumi、Natsume Takayoshi、Zahariev Alexandre、Blanc St?phane、Hanya Goro
    • Journal Title

      Journal of Thermal Biology

      Volume: 76 Pages: 139-146

    • DOI

      10.1016/j.jtherbio.2018.07.009

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Improving the standards for gut microbiome analysis of fecal samples: insights from the field biology of Japanese macaques on Yakushima Island2018

    • Author(s)
      Hayakawa Takashi、Sawada Akiko、Tanabe Akifumi S.、Fukuda Shinji、Kishida Takushi、Kurihara Yosuke、Matsushima Kei、Liu Jie、Akomo-Okoue Etienne-Francois、Gravena Waleska、Kashima Makoto、Suzuki Mariko、Kadowaki Kohmei、Suzumura Takafumi、Inoue Eiji、Sugiura Hideki、Hanya Goro、Agata Kiyokazu
    • Journal Title

      Primates

      Volume: 59 Issue: 5 Pages: 423-436

    • DOI

      10.1007/s10329-018-0671-x

    • NAID

      40021661058

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ethical issues concerning wildlife research in Japan and its perspective: preface2018

    • Author(s)
      森光 由樹、梶 光一
    • Journal Title

      Honyurui Kagaku (Mammalian Science)

      Volume: 58 Issue: 2 Pages: 253-255

    • DOI

      10.11238/mammalianscience.58.253

    • NAID

      130007583789

    • ISSN
      0385-437X, 1881-526X
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ethical issues and prospects of primate field research—Guidelines of the international primate society and the problems of Japanese primate studies—2018

    • Author(s)
      森光 由樹
    • Journal Title

      Honyurui Kagaku (Mammalian Science)

      Volume: 58 Issue: 2 Pages: 265-273

    • DOI

      10.11238/mammalianscience.58.265

    • NAID

      130007584292

    • ISSN
      0385-437X, 1881-526X
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Next-generation sequencing-based miRNA expression analysis in Parp1-deficient embryonic stem cell-derived exosomes.2018

    • Author(s)
      Nozaki Tadashige、Sasaki Yuka、Fukuda Itsuko、Isumi Mayu、Nakamoto Keitaro、Onodera Takae、Masutani Mitsuko
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 499 Issue: 3 Pages: 410-415

    • DOI

      10.1016/j.bbrc.2018.03.073

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure2018

    • Author(s)
      Sasaki Daisuke、Sasaki Kengo、Ikuta Naoko、Yasuda Takahiro、Fukuda Itsuko、Kondo Akihiko、Osawa Ro
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 435-435

    • DOI

      10.1038/s41598-017-18877-8

    • NAID

      120006394631

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Damage Control Strategies Affecting Crop-Raiding Japanese MacaqueBehaviors in a Farming Community2018

    • Author(s)
      Yosuke Ueda, Mieko Kiyono, Takanori Nagano, Shota Mochizuki, Takuhiko Murakami
    • Journal Title

      Human Ecology

      Volume: 46 Issue: 2 Pages: 259-268

    • DOI

      10.1007/s10745-018-9994-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Morphometric and Genetic Determination of Age Class and Sex for Fecal Pellets of Sika Deer (Cervus nippon)2017

    • Author(s)
      Hanya Goro、Naito Senri、Namioka Erina、Ueda Yosuke、Sato Yo、Pastrana Josue Alejandro、He Tianmeng、Yan Xiaochan、Saito Miho、Costa Raquel Filomena Pereira、Allanic Morgane、Honda Takeaki、Kurihara Yosuke、Yumoto Takakazu、Hayakawa Takashi
    • Journal Title

      Mammal Study

      Volume: 42 Issue: 4 Pages: 1-8

    • DOI

      10.3106/041.042.0406

    • NAID

      210000182462

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparison of energy balance between two different-sized groups of Japanese macaques (Macaca fuscata yakui)2017

    • Author(s)
      Kurihara Yosuke、Hanya Goro
    • Journal Title

      Primates

      Volume: 58 Issue: 3 Pages: 413-422

    • DOI

      10.1007/s10329-017-0607-x

    • NAID

      40021236811

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] ニホンザル保全管理を担う人材育成の実践2020

    • Author(s)
      清野未恵子
    • Organizer
      第36回日本霊長類学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Fermentation ability of Japanese macaques’ gut microbiome fluctuates seasonally with diet2020

    • Author(s)
      LEE Wanyi , HE Tianmeng, KURIHARA Yosuke, HANYA Goro
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 屋久島のニホンザルの腸内細菌の発酵能力:上部域と海岸部の比較2019

    • Author(s)
      *半谷 吾郎, Tackmann Janko, 澤田 晶子, Pokharel Sanjeeta Sharma, Valdevino Gisele de Castro Maciel, 大塚 亮真, 黒木 康太, 峠 明杜, 馬渕 諒真, Liu Jie, 畠山 剛臣, 山崎 美紗子, 山崎 絵理, 伊津野 彩子, Christian von Mering, 清水-稲継 理恵, 早川 卓志, 清水 健太郎, 牛田 一成
    • Organizer
      第35回日本霊長類学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 飼育下チンパンジーにおける腸内細菌叢の多様性2019

    • Author(s)
      早川 卓志, 平田 聡
    • Organizer
      第35回日本霊長類学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Gut microbiome shift of Japanese macaques as a result of human encroachment2019

    • Author(s)
      W. Lee, T. Hayakawa, N. Yamabata, M. Kiyono, G. Hanya
    • Organizer
      Symposium of Integrative Biology: Biodiversity in Asia
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ニホンザル加害群による農地とその周辺での採食行動と現在の被害管理との関係2018

    • Author(s)
      清野 未恵子
    • Organizer
      第34回日本霊長類学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Gut Microbiome Shift of Japanese Macaques as a Result of Human Encroachment2018

    • Author(s)
      Wanyi LEE, 早川 卓志, 山端 直人, 清野 未恵子, 半谷 吾郎
    • Organizer
      第34回日本霊長類学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] TEMPORAL PATTERNS AND CROPRAIDING BEHAVIOR OF A TROOP OF JAPANESE MONKEYS LIVING IN FOREST-AGRICULTURE ECOTONES IN HYOGO, JAPAN2018

    • Author(s)
      Mieko KIYONO, Tomoki YAIDA, Daichi KUDO
    • Organizer
      27th Congress of the International Primatological Society
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gut microbiome shift of Japanese macaques as a result of human encroachment.2018

    • Author(s)
      W. Lee, T. Hayakawa, N. Yamabata, M. Kiyono, G. Hanya
    • Organizer
      The 6th Asia Primates Symposium & 5th Asian (Indochinese) Primates Conservation
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 兵庫県篠山市ニホンザル群のミクロスケールでの生息地利用2017

    • Author(s)
      清野 未恵子
    • Organizer
      第23回「野生生物と社会」学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Gut Microbe Shift of Japanese Macaques as a Result of Human Encroachment.2017

    • Author(s)
      Wanyi Lee, Takashi Hayakawa, Naoto Yamabata, Mieko Kiyono, Goro Hanya
    • Organizer
      第62回プリマーテス研究会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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