Establishment of myotube contractile force and the molecular mechanisms underlying muscle atrophy
| Project/Area Number |
17H02159
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
Manabe Yasuko 東京都立大学, 人間健康科学研究科, 准教授 (60467412)
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| Co-Investigator(Kenkyū-buntansha) |
出口 真次 大阪大学, 基礎工学研究科, 教授 (30379713)
古市 泰郎 東京都立大学, 人間健康科学研究科, 助教 (40733035)
松井 翼 大阪大学, 基礎工学研究科, 講師 (50638707)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | 骨格筋 / 筋収縮 / 張力 / 初代培養細胞 / 細胞・組織 / 収縮 |
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| Outline of Final Research Achievements |
The purpose of this study is to establish the evaluation system for myotube contractility using locally formed micro-wrinkles on the silicon substrate. Mouse skeletal muscle primary myoblasts were seeded on the silicon substrate with the appropriate hardness adjusted for skeletal muscle cells and differentiated to the myotubes. When the myotubes were stimulated by electric pulse, the wrinkles formed on the silicon substrate in response to myotube contraction. The force index as an indicator of muscle contractile force was calculated by the total wrinkle length of the first three contractions. In this system, the contractile force of atrophy model myotubes was significantly reduced, while that of hypertrophy myotubes was significantly increased. These results show that our proposed myotube contractility assay system is suitable to assess muscle quality as an index of contractile force.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、シリコーン上に蒔いた筋細胞を分化させ、電気刺激により細胞が収縮する時にできるシワの長さを計測することで、細胞の収縮力を評価する系を構築した。これまで、骨格筋細胞で収縮力を測定する方法はなく、本研究で細胞の収縮力を簡便かつ客観的に測定できる系を構築できたことの学術的意義は大きい。さらに本研究で構築した系を用いると、筋萎縮を抑制したり、筋の発揮張力を増強する食品成分・薬剤のスクリーニングにも応用することでき、社会的な意義は大きい。
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Report
(5 results)
Research Products
(57 results)
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[Journal Article] Effect of antioxidant supplementation on skeletal muscle and metabolic profile in aging mice.2021
Author(s)
Tsukamoto-Sen S, Kawakami S, Maruki-Uchida H, Ito R, Matsui, Komiya Y, Mita Y, Morisasa M, Goto-Inoue N, Furuichi, Manabe Y, Morita M, Fujii NL.
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Journal Title
Food Funct.
Volume: 12
Issue: 2
Pages: 825-833
DOI
Related Report
Peer Reviewed
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[Journal Article] Monitoring and mathematical modeling of mitochondrial ATP in myotubes at single-cell level reveals two distinct population with different kinetics2020
Author(s)
N. Matsuda, K. Hironaka, M. Fujii, T. Wada, K. Kunida, H. Inoue, M. Eto, D. Hoshino, Y. Furuichi, Y. Manabe, N. L. Fujii, H. Noji, H. Imamura, S. Kuroda
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Journal Title
Quantitative Biology
Volume: 8
Issue: 3
Pages: 228-237
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Single-Cell Information Analysis Reveals That Skeletal Muscles Incorporate Cell-to-Cell Variability as Information Not Noise.2020
Author(s)
Wada T, Hironaka KI, Wataya M, Fujii M, Eto M, Uda S, Hoshino D, Kunida K, Inoue H, Kubota H, Takizawa T, Karasawa Y, Nakatomi H, Saito N, Hamaguchi H, Furuichi Y, Manabe Y, Fujii NL, Kuroda S.
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Journal Title
Cell Rep.
Volume: 32
Pages: 108051-108051
Related Report
Peer Reviewed / Open Access
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[Journal Article] R3hdml regulates satellite cell proliferation and differentiation2019
Author(s)
Sakamoto Kenichi、Furuichi Yasuro、Yamamoto Masashi、Takahashi Megumi、Akimoto Yoshihiro、Ishikawa Takahiro、Shimizu Takahiko、Fujimoto Masanori、Takada‐Watanabe Aki、Hayashi Aiko、Mita Yoshitaka、Manabe Yasuko、Fujii Nobuharu L、Ishibashi Ryoichi、Maezawa Yoshiro、Betsholtz Christer、Yokote Koutaro、Takemoto Minoru
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Journal Title
EMBO reports
Volume: 20
Issue: 11
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] 新規遺伝子R3hdmlは筋衛星細胞の増殖能を制御し、骨格筋の分化再生を促進する2018
Author(s)
坂本 憲一, 竹本 稔, 古市 泰郎, 高橋 恵, 秋元 義弘, 山本 雅, 石川 崇広, 前澤 善朗, 清水 孝彦, 眞鍋 康子, 藤井 宣晴, 横手 幸太郎
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Journal Title
日本内分泌学会雑誌
Volume: 94
Pages: 292-292
Related Report
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[Presentation] Single-cell information analysis reveals small intracellular and large intercellular variations increase cellular information capacity2019
Author(s)
Takumi Wada, Mitsutaka Wataya, Masashi Fujii, Ken-ichi Hironaka, Miki Eto, Shinsuke Uda, Daisuke Hoshino, Katsuyuki Kunida, Haruki Inoue, Hiroyuki Kubota Hiroki, Hamaguchi, Yasuro Furuichi , Yasuko Manabe, Nobuharu L. Fujii, Shinya Kuroda
Organizer
The 20th International Conference on System Biology (ICSB2019I)
Related Report
Int'l Joint Research
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[Presentation] 新規筋衛星細胞発現遺伝子R3hdmlは筋衛星細胞の増殖能を制御し、骨格筋の分化再生を促進する2018
Author(s)
坂本憲一, 竹本稔, 古市泰郎, 高橋恵, 秋元義弘, 山本雅, 石川崇広, 前澤善朗, 清水孝彦, 眞鍋康子, 藤井宣晴, 横手幸太郎
Organizer
第61回日本糖尿病学会
Related Report
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[Presentation] R3h domain containing-like (R3hdml) has crucial roles in skeletal muscle development and regeneration through the regulation of satellite cell proliferation.2017
Author(s)
Kenichi Sakamoto, Minoru Takemoto*, Yasuro Furuichi, Megumi Takahashi, Yoshihiro Akimoto, Masashi Yamamoto, Takahiro Ishikawa, Takahiko Shimizu, Yasuko Manabe, Nobuharu Fujii, Yoshiro Maezawa, and Koutaro Yokote
Organizer
Keystone Symposia (Aging and Mechanisms of Aging-Related Disease), Yokohama
Related Report
Int'l Joint Research
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