The physical fitness study on the crosstalk between skeletal muscle and gut with a focus on metabolism, myokine, and intestine barrier

• Project/Area Number: 17H02176
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied health science
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Aoi Wataru 京都府立大学, 生命環境科学研究科, 准教授 (60405272)
• Co-Investigator(Kenkyū-buntansha): 内藤 裕二 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00305575) ; 佐久間 邦弘 東京工業大学, リベラルアーツ研究教育院, 教授 (60291176)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2019: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 骨格筋 / 運動 / 腸内環境 / マイオカイン / 代謝 / エネルギー代謝 / 腸バリア / 腸内細菌

Outline of Final Research Achievements

This study examined the interactions between intestine barrier function, microbiota and metabolic/secretory function of skeletal muscle. We found that impairment of intestine barrier function caused glucose intolerance with muscle metabolic dysfunction in experimental mouse models. In addition, in the mice transplanted with microbiota from trained mice, metabolic/secretory factors in skeletal muscle were enhanced. On the other hand, in the mice with impaired muscle metabolism, myokine secretory- and intestinal barrier- functions were suppressed. These results support the concept of organ communication between skeletal muscle and gut.

Academic Significance and Societal Importance of the Research Achievements

本研究により，腸バリア機能，腸内細菌の変化は骨格筋の代謝を調整すること，逆に骨格筋の代謝・分泌機能は腸管免疫機能に影響を与えることが示され，腸内環境と骨格筋は血液を介して互いに協調しあっていることが明らかになった。体力向上，健康づくりにおいて，腸と骨格筋がともに良好な機能を保持することが重要であり，このことを考慮した運動習慣，食習慣を検討することが有意義と考えられる。

Research Products

• [Int'l Joint Research] カロリンスカ研究所(スウェーデン)
• [Int'l Joint Research] カロリンスカ研究所(スウェーデン)
• [Int'l Joint Research] Karolinska Institutet(スウェーデン)
• [Journal Article] Combined intake of astaxanthin, β-carotene, and resveratrol elevates protein synthesis during muscle hypertrophy in mice (2020)
  • Author(s): Kawamura, A., Aoi, W., Abe, R, Kobayashi, Y., Wada, S., Kuwahata, M., Higashi, A.
  • Journal Title: Nutrition Volume: 69 Pages: 110561-110561
  • DOI: 10.1016/j.nut.2019.110561
  • Peer Reviewed
• [Journal Article] Body fluid pH balance in metabolic health and possible benefits of dietary alkaline foods (2020)
  • Author(s): Aoi, W., Zou, X., Xiao, J.B., Marunaka, Y.
  • Journal Title: eFood J. Volume: 1 Pages: 12-23
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Combined treatment of dipeptidyl peptidase-4 inhibitor and exercise training improves lipid profile in KK/Ta mice (2019)
  • Author(s): Tanimura Yuko、Aoi Wataru、Mizushima Katsura、Higashimura Yasuki、Naito Yuji
  • Journal Title: Exp. Physiol. Volume: 104 Pages: 1051-1060
  • DOI: 10.1113/ep087449
  • Peer Reviewed
• [Journal Article] Lower birth weight is associated with higher sprint performance in female university students (2019)
  • Author(s): Abe, R., Aoi, W., Harada, K., Iwasa, M., Saruwatari, A., Odani, K., Yoshii, K., Ito, C., Ohmi, N., Takayama, Y., Nishikawa, K., Wada, S., Higashi, A.
  • Journal Title: JPFSM Volume: 8 Issue: 3 Pages: 117-125
  • DOI: 10.7600/jpfsm.8.117
  • NAID: 130007650941
  • ISSN: 2186-8123, 2186-8131
  • Peer Reviewed / Open Access
• [Journal Article] Secreted protein acidic and rich in cysteine (SPARC) improves glucose tolerance via AMP-activated protein kinase activation (2019)
  • Author(s): Aoi, W., Hirano, N., Lassiter, D.G., Björnholm, M., Chibalin, A.V., Sakuma, K., Tanimura, Y., Mizushima, K., Takagi, T., Naito, Y., Zierath, J.R., Krook, A.
  • Journal Title: The FASEB Journal Volume: 33 Pages: 10551-10562
  • DOI: 10.1096/fj.201900453r
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Aging-associated impairment in metabolic compensation by subcutaneous adipose tissue promotes diet-induced fatty liver disease in mice. (2019)
  • Author(s): Taketani Hiroyoshi、Nishikawa Tichiro、Nakajima Hisakazu、Kodo Kazuki、Sugimoto Satoru、Aoi Wataru、 Horike Shin-Ichi、 Meguro-Horike Makiko、 Ishiba Hiroshi、 Seko Yuya、Umemura Atsushi、 Yamaguchi Kanji、Moriguchi Michihisa、 Yasui Kohichiroh、 Itoh Yoshito.
  • Journal Title: Diabetes Metab Syndr Obes. Volume: 12 Pages: 1473-1492
  • DOI: 10.2147/dmso.s214093
  • Peer Reviewed / Open Access
• [Journal Article] Effect of downhill walking on next-day muscle damage and glucose metabolism in healthy young subjects (2019)
  • Author(s): Nakayama, A., Aoi, W., Takami, M., Hirano, N., Ogaya, Y., Wada, S., Higashi, A.
  • Journal Title: J. Physiol. Sci. Volume: 69 Pages: 31-38
  • DOI: 10.1007/s12576-018-0614-8
  • NAID: 40021804632
  • Peer Reviewed
• [Journal Article] Effect of dietary antioxidant-rich foods combined with aerobic training on energy metabolism in healthy young men (2019)
  • Author(s): Takami, M., Aoi W., Terajima, H., Tanimura, Y., Wada, S., Higashi, A.
  • Journal Title: J. Clin. Biochem. Nutr. Volume: 64 Issue: 1 Pages: 79-85
  • DOI: 10.3164/jcbn.18-40
  • NAID: 130007542253
  • ISSN: 0912-0009, 1880-5086
• [Journal Article] IL6 and LIF mRNA expression in skeletal muscle is regulated by AMPK and the transcription factors NFYC, ZBTB14 and SP1 (2018)
  • Author(s): Nylén, C., Aoi, W., Abdelmoez, A.M., Lassiter, D.G., Lundell, L.S., Wallberg-Henriksson, H., Näslund, E., Pillon, N.J., and Krook, A.
  • Journal Title: Am. J. Physiol. Endcrinol. Metab. Volume: 315
  • DOI: 10.1152/ajpendo.00398.2017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Secreted protein acidic and rich in cysteine functions in colitis via IL17A regulation in mucosal CD4+ T cells (2018)
  • Author(s): Tanaka M, Takagi T, Naito Y, Uchiyama K, Hotta Y, Toyokawa Y, Ushiroda C, Hirai Y, Aoi W, Higashimura Y, Mizushima K, Okayama T, Katada K, Kamada K, Ishikawa T, Handa O, Itoh Y.
  • Journal Title: J. Gastroenterol. Hepatol. Volume: 33 Pages: 671-680
  • DOI: 10.1111/jgh.13842
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of the effect of non-esterified and esterified astaxanthins on endurance performance in mice (2018)
  • Author(s): Aoi, W., Maoka, T., Abe, R., Fujishita, M., Tominaga, K.
  • Journal Title: J. Clin. Biochem. Nutr. Volume: 62 Issue: 2 Pages: 161-166
  • DOI: 10.3164/jcbn.17-89
  • NAID: 130006407479
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Presentation] 酸化チタンの摂取が腸管バリア機能および骨格筋糖代謝に及ぼす影響 (2019)
  • Author(s): 小谷日向子、青井渉、島田秋音、小林ゆき子、桑波田雅士
  • Organizer: 第73回日本栄養・食糧学会大会
• [Presentation] Intestinal microbiota mediates exercise-induced metabolic improvement through bile acids modification in mice (2019)
  • Author(s): Aoi, W.
  • Organizer: Cell Symposia Exercise Metabolism
  • Int'l Joint Research
• [Presentation] マウスにおける腸管バリア機能の破綻は耐糖能を低下させる (2019)
  • Author(s): 小谷日向子、青井渉、島田秋音、小林ゆき子、桑波田雅士
  • Organizer: 第58回日本栄養・食糧学会近畿支部大会
• [Presentation] The effects of milk casein hydrolysate and its derived peptides on glucose metabolism in C2C12 skeletal muscle cells (2019)
  • Author(s): Iwasa, M., Miyazaki, H.., Aoi, W.
  • Organizer: 13th Asian Congress of Nutrition
  • Int'l Joint Research
• [Presentation] Lactococcas lactis subsp. cremoris FC-fermented milk intake modulates energy metabolism in mouse skeletal muscle (2019)
  • Author(s): Aoi, W., Gotoh ,Y., Kosaka, H., Suzuki, T.
  • Organizer: International Conference of Food Factors 2019
  • Int'l Joint Research
• [Presentation] 若年女性における食事タイミングの違い及び運動習慣の有無が運動によるマイオカインの分泌応答に及ぼす影響 (2018)
  • Author(s): 西村佐保，青井渉，奥村なつみ，胡悠莉香，和田小依里，東あかね
  • Organizer: 第72回日本栄養・食糧学会大会
• [Presentation] Dietary antioxidants-contained foods promote skeletal muscle adaptation and reduce fatigue induced by resistance training (2018)
  • Author(s): Kawamura, A., Aoi, W., Wada, S., Higashi, A.
  • Organizer: American College of Sports Medicine 2018 Annual Meeting
  • Int'l Joint Research
• [Presentation] 運動トレーニングによる適応と酸化ストレス・レドックス管理 (2018)
  • Author(s): 青井渉
  • Organizer: 第73回日本体力医学会大会
  • Invited
• [Presentation] 若年女性における食事タイミングの違い及び運動習慣の有無が運動によるマイオカインSPARCの分泌応答に及ぼす影響 (2018)
  • Author(s): 西村佐保，青井渉，奥村なつみ，胡悠莉香，和田小依里，東あかね
  • Organizer: 第72回日本栄養・食糧学会大会
• [Presentation] Dietary antioxidants-contained foods promote skeletal muscle adaptation and reduce fatigue induced by resistance training (2018)
  • Author(s): Aki Kawamura, Wataru Aoi, Sayori Wada, Akane Higashi
  • Organizer: American College of Sports Medicine 2018 Annual Meeting
  • Int'l Joint Research
• [Presentation] 運動トレーニングによる生体適応と酸化ストレス管理 (2017)
  • Author(s): 青井渉
  • Organizer: 第70回日本酸化ストレス学会学術集会
  • Invited
• [Presentation] 複数の抗酸化食品の摂取が10週間のレジスタンス運動による筋力およびエネルギー代謝の適応におよぼす影響 (2017)
  • Author(s): 河村亜希，青井渉，和田小依里，東あかね
  • Organizer: 日本アミノ酸学会第11回学術大会
• [Presentation] 自発運動によるLactobacillus属の増加に対する食餌の影響 (2017)
  • Author(s): 谷村祐子，青井渉，井上亮，水島かつら，内藤裕二
  • Organizer: 第72回日本体力医学会大会
• [Book] Extreme and Rare Sports: Performance Demands, Drivers, Functional Foods, and Nutrition (2019)
  • Author(s): Aoi, W., Naito, Y.
  • Total Pages: 406
  • Publisher: Taylor & Francis Group
  • ISBN: 1138091448
• [Book] Dairy in human health and disease across the lifespan (2017)
  • Author(s): Iwasa, M., Aoi, W.
  • Total Pages: 490
  • Publisher: Elsevier
  • ISBN: 9780128098684