• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Femtosecond laser processing of functional fluoropolymer and microscopic observation method of 3D cultured cells on chip

Research Project

Project/Area Number 17H02794
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Optical engineering, Photon science
Research InstitutionHirosaki University

Principal Investigator

Hanada Yasutaka  弘前大学, 理工学研究科, 教授 (20435671)

Co-Investigator(Kenkyū-buntansha) 山田 勝也  弘前大学, 医学研究科, 准教授 (40241666)
澤田 英夫  弘前大学, 理工学研究科, 教授 (50259909)
武安 伸幸  岡山大学, 自然科学研究科, 准教授 (90373323)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsフェムト秒レーザー / 微細加工 / フッ素ポリマー / ナノファイバー / ナノ粒子 / バイオチップ / 回折格子 / 空間光位相変調器 / ナノ加工 / 低屈折率フッ素ポリマー / 細胞培養 / 変調fsレーザー / 低屈折率ポリマー / 細胞集塊 / バイオチップデバイス / 波面制御 / 濡れ性 / CYTOP / ダブルパルス
Outline of Final Research Achievements

We investigated the fundamental characteristics of CYTOP nanoparticle (NP) deposition by holographic femtosecond (fs) laser processing. The fs laser deposition revealed that interweaving fibrous network structures composed of NP aggregates and a monolayer structure of NPs can be easily formed on the glass slide. The wettability measurement revealed that the surface of the network structures behaves superhydrophobic. Consequently, the holographic fs laser processing of CYTOP NPs enabled the selective culturing of HeLa cells on the glass biochip.
In the meanwhile, laser ablation with a fs oscillator and a megahertz-order repetition rate enabled the high-quality microfabrication of CYTOP due to a heat accumulation effect at the laser irradiated areas. Fabrication of a diffraction grating was also performed by the fabrication of periodic microgrooves on the CYTOP substrate using the oscillator.

Academic Significance and Societal Importance of the Research Achievements

空間光位相変調器を用いて波面整形したfsレーザーのCYTOP加工により、ナノ粒子が凝集した網目構造やナノ粒子を単層分布させた表面形状をガラス基板上に堆積することに成功した。また、超撥水性を示すナノ粒子の網目構造を選択的に堆積させることで、所望する箇所に接着細胞(HeLa)が培養可能なガラスバイオチップ基板の作製にも成功している。更に、CYTOPのfsオシレーター加工の実現や未解明であったfsレーザー加工のメカニズム解明、各種レーザー加工の基本特性調査や汎用レーザーを用いたCYTOPの新規加工技術開発も行っており、高次機能バイオチップ開発を目指した微小光学デバイスの作製にも成功している。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (36 results)

All 2021 2020 2019 2018 2017

All Journal Article (13 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 13 results,  Open Access: 8 results) Presentation (21 results) (of which Int'l Joint Research: 9 results,  Invited: 11 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Nanoparticle deposition of fluoropolymer CYTOP via holographic femtosecond laser processing and its biochip application.2020

    • Author(s)
      Ozaki R, Ishida K, Morita E, Hanada Y
    • Journal Title

      Applied Sciences

      Volume: 10(20) Issue: 20 Pages: 7243-7243

    • DOI

      10.3390/app10207243

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microfabrication of UV-transparent fluoropolymer using high-repetition femtosecond oscillator2020

    • Author(s)
      Ozaki Ryo、Hanada Yasutaka
    • Journal Title

      Applied Physics A

      Volume: 126 Issue: 2 Pages: 116-120

    • DOI

      10.1007/s00339-019-3252-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single cell analysis of sinking diatoms studied using a homemade ‘tumbled’ optical microscope system2020

    • Author(s)
      Shoumura Shingo、Hamano Ryo、Hanada Yasutaka、Mayama Shigeki、Umemura Kazuo
    • Journal Title

      Journal of Microbiological Methods

      Volume: 168 Pages: 105804-105804

    • DOI

      10.1016/j.mimet.2019.105804

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid, high-quality microfabrication of the thermoset polymer PDMS using laser-induced bubbles2019

    • Author(s)
      Tomoya Naruse and Yasutaka Hanada
    • Journal Title

      Optics Express

      Volume: 27 Issue: 7 Pages: 9429-9438

    • DOI

      10.1364/oe.27.009429

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preparation of Amphiphobically Modified Poly(vinyl alcohol) Film by Fluoroalkyl End-Capped Vinyltrimethoxysilane Oligomer2019

    • Author(s)
      Y. Aomi and H. Sawada
    • Journal Title

      J. Coat. Technol. Res.

      Volume: 印刷中 Issue: 3 Pages: 651-660

    • DOI

      10.1007/s11998-018-0148-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of Fluoroalkyl End-Capped Oligomers/Hexagonal Boron Nitride Nanocomposites Possessing No Weight Loss Behavior in Nanocomposites Even after Calcination at 800℃2019

    • Author(s)
      Saengkaew Jittraporn、Ogasawara Takahumi、Yamashita Katsumi、Kongparakul Suwadee、Sawada Hideo
    • Journal Title

      Open Journal of Composite Materials

      Volume: 09 Issue: 02 Pages: 72-98

    • DOI

      10.4236/ojcm.2019.92004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of a platform for activatable fluorescent substrates of glucose transporters (GLUTs)2019

    • Author(s)
      Takasugi Tomohiro、Hanaoka Kenjiro、Sasaki Ayako、Ikeno Takayuki、Komatsu Toru、Ueno Tasuku、Yamada Katsuya、Urano Yasuteru
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 27 Issue: 10 Pages: 2122-2126

    • DOI

      10.1016/j.bmc.2019.02.055

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aberrant Uptake of a Fluorescent L-Glucose Analogue (fLG) into Tumor Cells Expressing Malignant Phenotypes2018

    • Author(s)
      Yamada Katsuya
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 41 Issue: 10 Pages: 1508-1516

    • DOI

      10.1248/bpb.b18-00089

    • NAID

      130007493314

    • ISSN
      0918-6158, 1347-5215
    • Year and Date
      2018-10-01
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Etching-Assisted Ablation of the UV-Transparent Fluoropolymer CYTOP Using Various Laser Pulse Widths and Subsequent Microfluidic Applications2018

    • Author(s)
      Nemoto Keisuke、Hanada Yasutaka
    • Journal Title

      Micromachines

      Volume: 9 Issue: 12 Pages: 662-662

    • DOI

      10.3390/mi9120662

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sub-50-as isolated extreme ultraviolet continua generated by 1.6-cycle near-infrared pulse combined with double optical gating scheme2018

    • Author(s)
      Katsuya Oguri, Hiroki Mashiko, Tatsuya Ogawa, Yasutaka Hanada, Hidetoshi Nakano, and Hideki Gotoh
    • Journal Title

      Applied Physics Letters

      Volume: 112 Issue: 18 Pages: 181105-181105

    • DOI

      10.1063/1.5027135

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of morphology-controlled fluoroalkyl end-capped vinyltrimethoxysilane oligomeric silica/magnesium oxide nanocomposite particles: Development of magnesium oxide nanocomposite particles possessing a water-resistance ability2018

    • Author(s)
      Y. Oikawa, Y. Goto, M. Nishida, and H. Sawada
    • Journal Title

      J. Sol-Gel Sci. Technol.

      Volume: 印刷中 Issue: 1 Pages: 318-329

    • DOI

      10.1007/s10971-018-4655-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surface Microfabrication of Conventional Glass Using Femtosecond Laser for Microfluidic Applications2017

    • Author(s)
      Takuma Niioka, Yasutaka Hanada
    • Journal Title

      International Journal of Automation Technology

      Volume: 11 Issue: 6 Pages: 878-882

    • DOI

      10.20965/ijat.2017.p0878

    • NAID

      130007505986

    • ISSN
      1881-7629, 1883-8022
    • Year and Date
      2017-11-05
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unknown biological effects of L-glucose, ALA, and PUFA2017

    • Author(s)
      Yamada, K., Sato, D., Nakamura, T., Amano, H., and Morimoto, Y.
    • Journal Title

      J. Physiol. Sci.

      Volume: 67 Issue: 5 Pages: 539-548

    • DOI

      10.1007/s12576-017-0544-x

    • NAID

      40021301945

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ホログラフィックフェムト秒レーザー加工によるフッ素ポリマーCYTOPのナノ粒子生成および細胞培養応用2021

    • Author(s)
      中平 敬太, 尾崎 諒,花田修賢
    • Organizer
      第68回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 紫外透過性フッ素樹脂のホログラフィックフェムト秒レーザー加工によるナノ粒子生成およびそのバイオチップ応用2020

    • Author(s)
      尾崎諒,花田修賢
    • Organizer
      2020年度精密工学会東北支部学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Microfabrication of UV-transparent polymer CYTOP using various lasers for biochip applications2019

    • Author(s)
      Yasutaka Hanada
    • Organizer
      Microfabrication of UV-transparent polymer CYTOP using various lasers for biochip applications” International Symposium on SSS Laser Processing (3S-LP)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高繰り返しフェムト秒レーザーを用いた紫外透過性ポリマーへの回折格子作製2019

    • Author(s)
      尾崎諒,花田修賢
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Brain area-dependent astrocyte heterogeneity detected in mice by dopamine receptor expressions2019

    • Author(s)
      長友克広、小林和人、山本欣郎、山田勝也
    • Organizer
      FAOPS2019・第96回日本生理学会大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低屈折率フッ素ポリマーを用いた超短パルスレーザーによる3次元バイオチップデバイス作製およびその応用2018

    • Author(s)
      花田修賢
    • Organizer
      第41回フッ素化学討論会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] フェムト秒レーザー3次元加工を用いたバイオチップ作製およびその応用2018

    • Author(s)
      花田修賢
    • Organizer
      2018年度精密工学会秋季大会シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 3D biochip fabrication of fluoric polymer CYTOP for clear microscopic observation at the fluid boundary2018

    • Author(s)
      Yasutaka Hanada
    • Organizer
      Progress on Ultrafast Laser Modifications of Materials (PULMM)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 超短パルスレーザを用いた機能性ポリマーの3次元バイオチップデバイス作製2018

    • Author(s)
      花田修賢
    • Organizer
      第89回レーザ加工学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 細胞分析を目的とした超短パルスレーザーによる低屈折率ポリマーの3次元加工技術2018

    • Author(s)
      竹内萌, 花田修賢
    • Organizer
      第62回光波センシング研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] フッ素系ポリマーのプラズモン誘起化学反応2018

    • Author(s)
      木内泰治, 武安 伸幸, 花田修賢
    • Organizer
      第79回応用物理学会学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 波面制御フェムト秒レーザー照射法による3次元微細構造体を用いた超撥水表面加工2018

    • Author(s)
      富田 麗菜, 花田修賢
    • Organizer
      2018年度精密工学会秋季大会学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Preparation and Property of Fluoroalkyl End-Capped Oligomer/Magnesium Oxide Nanocomposite2018

    • Author(s)
      H. Sawada
    • Organizer
      The Fifth International Conference of the Cis Countries (Sol-Gel Synthesis and Research of Inorganic Compounds, Hybrid Functional Materials and Disperse Systems - (SOL-GEL 2018))
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] luorescent L-glucose, a new tracer for imaging tumor cells having malignant potential.2018

    • Author(s)
      Yamada, K.
    • Organizer
      15th Meeting of the Asian-Pacific Society for Neurochemistry (APSN2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 瘍細胞が立体的に集積しスフェロイドを自発形成する新しい培養法2018

    • Author(s)
      小野幸輝、山田勝也
    • Organizer
      第50回東北生理談話会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光グルコースを用いたユーイング肉腫細胞の糖代謝イメージングGlucose metabolism imaging in cultured Ewing sarcoma cells using fluorescent glucose.2018

    • Author(s)
      小川哲也、大鹿周佐、佐々木綾子、小野浩弥、石橋恭之、山田勝也
    • Organizer
      第33回日本整形外科学会基礎学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 3D microfluidic fabrication using a low refractive index polymer for clear microscopic observation at the fluid boundary2018

    • Author(s)
      Y. Hanada
    • Organizer
      SPIE Photonics WEST 2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of glass-based biochip utilizing ultrashort pulse laser and its application2017

    • Author(s)
      Yasutaka Hanada
    • Organizer
      International Symposium on Micro-Nano Mechatronics and Human Science (MHS2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] フェムト秒レーザーによるバイオ応用マイクロチップの作製2017

    • Author(s)
      花田 修賢
    • Organizer
      第10回 バイオナノシステムズ研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Preparation of fluoroalkyl end-capped oligomeric composites possessing the superoleophilic/superhydrophobic and superoleophobic/superhydrophilic2017

    • Author(s)
      H. Sawada
    • Organizer
      International Conference on Advanced Materials and Building Materials (ICAMBM2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescent L-glucose (fLG) as a Potential Contrast Agent for Cancer Detection.2017

    • Author(s)
      Yamada, K.
    • Organizer
      International Drug Discovery Science and Technology - Japan 2017 (IDDST-Japan 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Patent(Industrial Property Rights)] 透明材料の加工方法2018

    • Inventor(s)
      花田修賢
    • Industrial Property Rights Holder
      弘前大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-000508
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 透明材料の加工方法2018

    • Inventor(s)
      花田 修賢
    • Industrial Property Rights Holder
      弘前大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2023-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi