| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
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| Outline of Final Research Achievements |
The alternation of intracellular metabolites cultured in plasma-activated solutions was observed in brain tumor cells (U251SPs). Bioenergetics and biomass production were taken place through catabolism from glucose uptake in the cell culture medium; (i) bioenergetics were deficient in the plasma-activated culture medium due to reduced activity of glycolytic enzymes due to oxidative stress; (ii) lactate-containing solutions enhanced anabolism from lactate uptake while maintaining a reduced intracellular state; and (iii) plasma-activated organics acted as inhibitors of glutamine assimilation and lipid metabolism in the plasma-activated lactate solution (PAL). In particular, we found that intracellular metabolism was altered by plasma-activated organic matter in PAL, leading to plasma-induced cell death.
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