Plasma Biochemistry and Metabolic Profiles of Cells Interacted with Non-thermal Plasmas

• Project/Area Number: 17H02805
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plasma electronics
• Research Institution: Kyushu University (2019); Nagoya University (2017-2018)
• Principal Investigator: Ishikawa Kenji 九州大学, プラズマナノ界面工学センター, 特任教授 (60417384)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2017: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
• Keywords: 低温プラズマ / 非平衡大気圧プラズマ / メタボローム / 細胞内代謝 / 活性酸素窒素種 / グリオーマ / プラズマ活性有機物 / 乳酸 / プラズマ医療 / 代謝 / 生体分子 / プラズマバイオ / 生物化学 / メタボリズム / 核磁気共鳴 / プラズマ / プラズマ医療科学 / プラズマ化学 / メタボローム解析 / ナノ材料 / ストレス

Outline of Final Research Achievements

The alternation of intracellular metabolites cultured in plasma-activated solutions was observed in brain tumor cells (U251SPs). Bioenergetics and biomass production were taken place through catabolism from glucose uptake in the cell culture medium; (i) bioenergetics were deficient in the plasma-activated culture medium due to reduced activity of glycolytic enzymes due to oxidative stress; (ii) lactate-containing solutions enhanced anabolism from lactate uptake while maintaining a reduced intracellular state; and (iii) plasma-activated organics acted as inhibitors of glutamine assimilation and lipid metabolism in the plasma-activated lactate solution (PAL). In particular, we found that intracellular metabolism was altered by plasma-activated organic matter in PAL, leading to plasma-induced cell death.

Academic Significance and Societal Importance of the Research Achievements

大気圧下でガス温度の上昇を伴わない非平衡（低温）プラズマの生成が制御されて可能となり，液体や生体をはじめとする様々な対象にプラズマの照射効果が見いだされていた中，本研究は，プラズマ活性溶液中で培養された細胞内のメタボローム解析を世界で初めて実施した，研究開始当初はプラズマが生成するRONSの影響が生体作用の主要な因子であるといった見解から，溶液中の有機物の変性をプラズマが液体に作用した結果に生じており、その溶液中に産生された成分が細胞の恒常的応答を変動させ、代謝産物における明瞭な違いを世界で初めて明らかにした．

Research Products

• [Journal Article] Electron spin resonance as a tool to monitor the influence of novel processing technologies on food properties (2020)
  • Author(s): Francisco J. Barba, Shahin Roohinejad, Kenji Ishikawa, Sze Ying Leong, Alaa El-Din A Bekhit, Jorge A. Saraiva, and Nikolai Lebovka
  • Journal Title: Trends in Food Science and Technology Volume: 100 Pages: 77-87
  • DOI: 10.1016/j.tifs.2020.03.032
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Oxidative stress-dependent and -independent death of glioblastoma cells induced by non-thermal plasma-exposed solutions (2019)
  • Author(s): Tanaka Hiromasa、Mizuno Masaaki、Katsumata Yuko、Ishikawa Kenji、Kondo Hiroki、Hashizume Hiroshi、Okazaki Yasumasa、Toyokuni Shinya、Nakamura Kae、Yoshikawa Nobuhisa、Kajiyama Hiroaki、Kikkawa Fumitaka、Hori Masaru
  • Journal Title: Sci Rep. Volume: 9 Pages: 13657-13657
  • DOI: 10.1038/s41598-019-50136-w
  • Peer Reviewed / Open Access
• [Journal Article] Simultaneous achievement of antimicrobial property and plant growth promotion using plasma‐activated benzoic compound solution (2019)
  • Author(s): Iwata Naoyuki、Gamaleev Vladislav、Hashizume Hiroshi、Oh Jun‐Seok、Ohta Takayuki、Ishikawa Kenji、Hori Masaru、Ito Masafumi
  • Journal Title: Plasma Processes and Polymers Volume: 16 Pages: 1900023-1900023
  • DOI: 10.1002/ppap.201900023
  • Peer Reviewed
• [Journal Article] Atmospheric Pressure Plasma-Treated Carbon Nanowalls’ Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (CNW-SALDI-MS) (2019)
  • Author(s): Ohta Takayuki、Ito Hironori、Ishikawa Kenji、Kondo Hiroki、Hiramatsu Mineo、Hori Masaru
  • Journal Title: C Journal of Carbon Research Volume: 5 Pages: 40-40
  • DOI: 10.3390/c5030040
  • Peer Reviewed / Open Access
• [Journal Article] Gene Expression of Osteoblast-like Cells on Carbon-Nanowall as Scaffolds during Incubation with Electrical Stimulation (2019)
  • Author(s): Ichikawa Tomonori、Kondo Hiroki、Ishikawa Kenji、Tsutsumi Takayoshi、Tanaka Hiromasa、Sekine Makoto、Hori Masaru
  • Journal Title: ACS Applied Bio Materials Volume: 2 Pages: 2698-2702
  • DOI: 10.1021/acsabm.9b00178
  • Peer Reviewed
• [Journal Article] Plasma-activated solution alters the morphological dynamics of supported lipid bilayers observed by high-speed atomic force microscopy (2019)
  • Author(s): Yamaoka Sotaro、Kondo Hiroki、Hashizume Hiroshi、Ishikawa Kenji、Tanaka Hiromasa、Hori Masaru
  • Journal Title: Appl. Phys. Express Volume: 12 Issue: 6 Pages: 066001-066001
  • DOI: 10.7567/1882-0786/ab1a58
  • NAID: 210000155782
  • ISSN: 1882-0778, 1882-0786
  • Peer Reviewed
• [Journal Article] Non-thermal plasma-activated medium modified metabolomic profiles in the glycolysis of U251SP glioblastoma (2019)
  • Author(s): Kurake Naoyuki、Ishikawa Kenji、Tanaka Hiromasa、Hashizume Hiroshi、Nakamura Kae、Kajiyama Hiroaki、Toyokuni Shinya、Kikkawa Fumitaka、Mizuno Masaaki、Hori Masaru
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 662 Pages: 83-92
  • DOI: 10.1016/j.abb.2018.12.001
  • Peer Reviewed
• [Journal Article] Systematic diagnostics of the electrical, optical, and physicochemical characteristics of low-temperature atmospheric-pressure helium plasma sources (2019)
  • Author(s): Keigo Takeda, Hiromasa Yamada, Kenji Ishikawa, Hajime Sakakita, Jaeho Kim, Masashi Ueda, Jun-ichiro Ikeda, Yoshihiro Akimoto, Yosky Kataoka, Naoaki Yokoyama, Yuzuru Ikehara, and Masaru Hori
  • Journal Title: Journal of Physics D: Applied Physics Volume: 52 Pages: 165202-165202
  • DOI: 10.1088/1361-6463/aaff44
  • Peer Reviewed
• [Journal Article] Effect of electrical stimulation on proliferation and bone-formation by osteoblast-like cells cultured on carbon nanowall scaffolds (2019)
  • Author(s): Tomonori Ichikawa, Suiki Tanaka, Hiroki Kondo, Kenji Ishikawa, Takayoshi Tsutsumi, Makoto Sekine, and Masaru Hori
  • Journal Title: Appl. Phys. Express Volume: 12 Issue: 2 Pages: 025006-025006
  • DOI: 10.7567/1882-0786/aaf469
  • NAID: 210000135543
  • ISSN: 1882-0778, 1882-0786
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of non-thermal plasma-induced effects in cancer cells (2019)
  • Author(s): Tanaka Hiromasa、Mizuno Masaaki、Ishikawa Kenji、Toyokuni Shinya、Kajiyama Hiroaki、Kikkawa Fumitaka、Hori Masaru
  • Journal Title: Biological Chemistry Volume: 400 Pages: 87-91
  • DOI: 10.1515/hsz-2018-0199
  • NAID: 120006577057
  • Peer Reviewed
• [Journal Article] New Hopes for Plasma-Based Cancer Treatment (2018)
  • Author(s): Tanaka Hiromasa、Mizuno Masaaki、Ishikawa Kenji、Toyokuni Shinya、Kajiyama Hiroaki、Kikkawa Fumitaka、Hori Masaru
  • Journal Title: Plasma Volume: 1 Pages: 150-155
  • DOI: 10.3390/plasma1010014
  • Peer Reviewed
• [Journal Article] Cytotoxic effects of plasma-irradiated fullerenol (2018)
  • Author(s): Kanno Daiki、Tanaka Hiromasa、Ishikawa Kenji、Hashizume Hiroshi、Hori Masaru
  • Journal Title: Journal of Physics D: Applied Physics Volume: 51 Pages: 375401-375401
  • DOI: 10.1088/1361-6463/aad510
  • Peer Reviewed
• [Journal Article] Cell Deposition Microchip with Micropipette Control over Liquid Interface Motion, Cell Medicine (2018)
  • Author(s): D. Onoshima, Y. Hattori, H. Yukawa, K. Ishikawa, M. Hori, Y. Baba
  • Journal Title: Cell Medicine Volume: 10 Pages: 1-5
  • DOI: 10.1177/2155179017733152
  • Peer Reviewed
• [Journal Article] Low-autofluorescence fluoropolymer membrane filters for cell filtration (2018)
  • Author(s): Kihara Naoto、Kuboyama Daiki、Onoshima Daisuke、Ishikawa Kenji、Tanaka Hiromasa、Ozawa Naoya、Hase Tetsunari、Koguchi Ryohei、Yukawa Hiroshi、Odaka Hidefumi、Hasegawa Yoshinori、Baba Yoshinobu、Hori Masaru
  • Journal Title: Japanese Journal of Applied Physics Volume: 57 Issue: 6S2 Pages: 06JF03-06JF03
  • DOI: 10.7567/jjap.57.06jf03
  • NAID: 210000149208
  • ISSN: 0021-4922, 1347-4065
  • Peer Reviewed / Open Access
• [Journal Article] Free radical generation by non-equilibrium atmospheric pressure plasma in alcohol-water mixtures. An EPR-spin trapping study (2018)
  • Author(s): Hidefumi Uchiyama, Qing-Li Zhao, Gabor Andocs, Nobuyuki Nojima, Keigo Takeda, Murali Krishna, Yuji Matsuya, Kenji Ishikawa, Masaru Hori, Kyo Noguchi, and Takashi Kondo
  • Journal Title: J. Phys.D: Appl. Phys. Volume: 51 Pages: 026201-026201
  • DOI: 10.1088/1361-6463/aaab09
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Glioblastoma cell lines display different sensitivities to plasma-activated medium (2018)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Hiroaki Kajiyama, Fumitaka Kikkawa, and Masaru Hori
  • Journal Title: IEEE Plasma Radiation and Plasma Medical Sciences Volume: 2 Pages: 99-102
  • DOI: 10.1109/trpms.2017.2721973
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cytotoxicity on cancer HeLa cells sensitively against normal MCF10A cells in cultivations with cell culture medium treated by microwave-excited atmospheric pressure plasmas (2018)
  • Author(s): Yohei Takahashi, Yusuke Taki, Keigo Takeda, Hiroshi Hashizume, Hiromasa Tanaka, Kenji Ishikawa, and Masaru Hori
  • Journal Title: J. Phys.D: Appl. Phys. Volume: 51 Pages: 095202-095202
  • DOI: 10.1088/1361-6463/aaa885
  • Peer Reviewed
• [Journal Article] Reduced HeLa cell viability in methionine-containing cell culture medium irradiated with microwave-excited atmospheric-pressure plasma (2018)
  • Author(s): Yohei Takahashi, Yusuke Taki, Keigo Takeda, Hiroshi Hashizume, Hiromasa Tanaka, Kenji Ishikawa, and Masaru Hori
  • Journal Title: Plasma Processes Polymers Volume: 15 Pages: 1700200-1700200
  • DOI: 10.1002/ppap.201700200
  • Peer Reviewed
• [Journal Article] Selective production of ROS and RNS in the plasma treated water by using nonthermal low- and high-frequency plasma jets (2018)
  • Author(s): Giichiro Uchida, Kosuke Takenaka, Keigo Takeda, Kenji Ishikawa, Masaru Hori, Yuichi Setsuhara
  • Journal Title: Japanese Journal of Applied Physics Volume: 57 Issue: 1 Pages: 0102B4-0102B4
  • DOI: 10.7567/jjap.57.0102b4
  • ISSN: 0021-4922, 1347-4065
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Plasma-Activated Lactated Ringer's Solution on Pancreatic Cancer Cells In Vitro and In Vivo. (2018)
  • Author(s): Sato Y, Yamada S, Takeda S, Hattori N, Nakamura K, Tanaka H, Mizuno M, Hori M, Kodera Y
  • Journal Title: Ann. Surg. Oncol Volume: 25 Pages: 23-30
  • DOI: 10.1245/s10434-017-6239-y
  • Peer Reviewed
• [Journal Article] Intracellular-molecular changes in plasma-irradiated budding yeast cells studied using multiplex coherent anti-Stokes Raman scattering microscopy (2017)
  • Author(s): Ryo Furuta, Naoyuki Kurake, Kenji Ishikawa, Keigo Takeda, Hiroshi Hashizume, Hiroki Kondo, Takayuki Ohta, Masafumi Ito, Makoto Sekine, and Masaru Hori
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 19 Pages: 13438-13442
  • DOI: 10.1039/c7cp00489c
  • NAID: 120006337722
  • Peer Reviewed
• [Journal Article] Intracellular responses to reactive oxygen and nitrogen species, and lipid peroxidation: Induction of apoptotic death in HeLa cells following cultivation in non-equilibrium (cold) atmospheric pressure plasma-activated medium (2017)
  • Author(s): Ryo Furuta, Naoyuki Kurake, Kenji Ishikawa, Keigo Takeda, Hiromasa Tanaka, Hiroshi Hashizume, Hiroki Kondo, Makoto Sekine and Masaru Hori
  • Journal Title: Plasma Process and Polymers Volume: 14
  • DOI: 10.1002/ppap.201700123
  • Peer Reviewed
• [Journal Article] Oxygen-radical pretreatment promotes cellulose degradation by cellulolytic enzymes (2017)
  • Author(s): Sakai Kiyota、Kojiya Saki、Kamijo Junya、Tanaka Yuta、Tanaka Kenta、Maebayashi Masahiro、Oh Jun-Seok、Ito Masafumi、Hori Masaru、Shimizu Motoyuki、Kato Masashi
  • Journal Title: J Appl Phys Volume: 10 Pages: 21330-21330
  • DOI: 10.1186/s13068-017-0979-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] State of the art in medical applications using non-thermal atmospheric pressure plasma (2017)
  • Author(s): Hiromasa Tanaka, Kenji Ishikawa, Masaaki Mizuno, Shinya Toyokuni, Hiroaki Kajiyama, Fumitaka Kikkawa, Hans-Robert Metelmann, and Masaru Hori
  • Journal Title: Rev. Mod. Plasma Phys. Volume: 1 Pages: 3-3
  • DOI: 10.1007/s41614-017-0004-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Spatial distributions of O, N, NO, OH and vacuum ultraviolet light along gas flow direction in an AC-excited atmospheric pressure Ar plasma jet generated in open air (2017)
  • Author(s): Keigo Takeda, Kenji Ishikawa, Hiromasa Tanaka, Makoto Sekine, and Masaru Hori
  • Journal Title: J. Phys. D: Appl. Phys. Volume: 50 Pages: 195202-195202
  • DOI: 10.1088/1361-6463/aa6555
  • Peer Reviewed
• [Journal Article] Cold atmospheric helium plasma causes synergistic enhancement in cell death with hyperthermia and an additive enhancement with radiation (2017)
  • Author(s): Moniruzzaman Rohan, Qing-Li Zhao, Paras Jawaid, Keigo Takeda, Kenji Ishikawa, Masaru Hori, Kei Tomihara, Noguchi Kyo, Takashi Kondo, and Makoto Noguchi
  • Journal Title: Sci Rep Volume: 7 Pages: 11659-11659
  • DOI: 10.1038/s41598-017-11877-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Crystallization of calcium oxalate dihydrate in a buffered calcium-containing glucose solution by irradiation with non-equilibrium atmospheric pressure plasma (2017)
  • Author(s): Naoyuki Kurake, Hiromasa Tanaka, Kenji Ishikawa, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Yuzuru Ikehara, and Masaru Hori
  • Journal Title: J Appl Phys Volume: 122 Pages: 143301-143301
  • DOI: 10.1063/1.5006598
  • Peer Reviewed
• [Presentation] プラズマ生成機能性窒素ラジカル誘起の連鎖反応の統合解析に向けて (2020)
  • Author(s): 石川 健治
  • Organizer: 第３３回プラズマ新領域研究会
  • Invited
• [Presentation] Germination kinetics of plasma-activated seeds of lettuce lactuca sativa (2020)
  • Author(s): Kenji Ishikawa, Ryoya Sato, Kayo Matsuo, Hayate Tanaka, Masaya Hiromatsu, Ryo Arita, Tomoaki Yoshida, Pankaj Attri, Kunihiro Kamataki, Naho Itagaki, Daisuke Yamashita, Kazunori Koga, and Masaharu Shiratani
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Non-Equilibrium Atmospheric Pressure Plasma-Activated Organic Solutions (2020)
  • Author(s): Yang Liu, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Fumitaka Kikkawa, Kae Nakamura, Masaaki Mizuno, Shinya Toyokuni, and Masaru Hori
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Effect of Non-Equilibrium Atmospheric Pressure Plasma on Alcohol Fermentation of Budding Yeast (2020)
  • Author(s): Shogo Matsumura, Hiromasa Tanaka, Hiroshi Hashizume, Kenji Ishikawa, and Masaru Hori
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Ionization Enhancement Using CNWs in Laser Desorption / Ionization Mass Spectrometry (2020)
  • Author(s): Ryusei Sakai, Hiroki Kondo, Kenji Ishikawa, Takayuki Ohta, Mineo Hiramatsu, Makoto Sekine, and Masaru Hori
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Measurement of Spatial Distribution of Absolute Hydrogen Radical Density in Non- Equilibrium Atmospheric Pressure Plasma by Vacuum Ultraviolet Absorption Spectroscopy (2020)
  • Author(s): Kaede Katsuno, Takayoshi Tsutsumi, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Plasma-Activated Medium Induced Oxidative Stress-Dependent Cell Death on Glioblastoma Cells (2020)
  • Author(s): Masaru Hori, Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, Yasumasa Okazaki, Shinya Toyokuni and Fumitaka Kikkawa
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Molecular Structure Analysis on Surface of Glucose Film Using Sum Frequency Generation Spectroscopy (2020)
  • Author(s): Yuta Yoshida, Takayuki Ohta, Kenji Ishikawa and Masaru Hori
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Extracellular Flux Analysis of Mitochondrial Stress on HeLa Cells in Plasma Activated Ringer’s Lactate Solution (PAL) (2020)
  • Author(s): Shogo Maeda, Hiromasa Tanaka, Kae Nakamura, Hiroshi Hashizume, Kenji Ishikawa, Kinji Ohno, Mikako Ito, Masaaki Mizuno, Fumitaka Kikkawa and Masaru Hori
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Enhancement of Intracellular Lipids Accumulation in Plasma-Treated Coccomyxa sp. (2020)
  • Author(s): Takumi Kato, Hiromasa Tanaka, Hiroshi Hashizume, Kenji Ishikawa and Masaru Hori
  • Organizer: ISPlasma2010/IC-PLANTS2020, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] In-liquid plasma formation at low temperature of in situ binding SnO2/graphene (2019)
  • Author(s): Kenji Ishikawa, Rajit R. Borude, Hirotsugu Sugiura, Takayoshi Tsutsumi, Hiroki Kondo, Nobuyuki Ikarashi, and Masaru Hori
  • Organizer: 10th International workshop on microplasma (IWM)
  • Int'l Joint Research / Invited
• [Presentation] Physics and chemistry in low-temperature plasma bioapplications (2019)
  • Author(s): Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Makoto Sekine, Fumitaka Kikkawa, Masaaki Mizuno, and Masaru Hori
  • Organizer: The 7th East Asia Joint Symposium on Plasma and Electrostatics Technologies for Environmental Applications (EAPETEA-7)
  • Int'l Joint Research / Invited
• [Presentation] プラズマ誘起有機酸の前駆体が拓く超バイオ機能 (2019)
  • Author(s): 石川 健治, 橋爪 博司, 田中 宏昌, 吉武 淳, 柴田 貴広, 小鹿一, 伊藤 昌文, 古閑 一憲, 白谷 正治, 豊國 伸哉, 吉川 史隆, 水野 正明, 堀 勝
  • Organizer: 秋季応用物理学会（北海道大学）
  • Invited
• [Presentation] 低温プラズマの大気圧下での発生・発生源から生体への活性種輸送 (2019)
  • Author(s): 石川 健治, 田中 宏昌, 豊國 伸哉, 吉川 史隆, 水野 正明, 堀 勝
  • Organizer: 第７２ 回日本酸化ストレス学会（札幌）
  • Invited
• [Presentation] プラズマ誘起液相ラジカル連鎖反応 (2019)
  • Author(s): 石川 健治, 堀勝，古閑一憲，白谷正治
  • Organizer: 第35回 九州・山口プラズマ研究会（対馬）
  • Invited
• [Presentation] Novel bactericidal method for aquaculture using benzoic-compound solutions treated with oxygen radicals (2019)
  • Author(s): Naoyuki Iwata, Vladislav Gamaleeve, Jun-Seok Oh, Hiroshi Hashizume, Takayuki Ohta, Kenji Ishikawa, Masaru Hori, and Masafumi Ito
  • Organizer: 24th International Symposium on Plasma Chemistry (ISPC24)
  • Int'l Joint Research
• [Presentation] Gene expression dynamics of glioblastoma cells in plasma-activated medium and plasma-activated Ringer’s lactate solution (2019)
  • Author(s): Hiromasa Tanaka, Masaru Hori, Masaaki Mizuno, Kenji Ishikawa, H. Kondo, H. Hashizume, T. Tsutsumi, K. Nakamura, N. Yoshikawa, H. Kajiyama, Shinya Toyokuni, and Fumitaka Kikkawa
  • Organizer: 24th International Symposium on Plasma Chemistry (ISPC24)
  • Int'l Joint Research
• [Presentation] Bactericidal activity in oxygen-radical-activated water (2019)
  • Author(s): Naoyuki Iwata, Vladislav Gamaleeve, Jun-Seok Oh, Kenji Ishikawa, Masaru Hori, and Masafumi Ito
  • Organizer: 24th International Symposium on Plasma Chemistry (ISPC24)
  • Int'l Joint Research
• [Presentation] Surface reaction analysis of plasma-treated bio-molecules using sum frequency generation spectroscopy (2019)
  • Author(s): Takayuki Ohta, Ryoya Katsuya, Kenji Ishikawa, and Masaru Hori
  • Organizer: 24th International Symposium on Plasma Chemistry (ISPC24)
  • Int'l Joint Research
• [Presentation] Enhancement of alcohol production of budding yeasts by treatment of nonequilibrium atmospheric pressure plasma (2019)
  • Author(s): Shogo Matsumura, Kenji Ishikawa, H. Tanaka, H. Hashizume, M. Ito, M. Hori
  • Organizer: XXXIV International Conference on Phenomena in Ionized Gases (XXXIV ICPIG) and the 10th International Conference on Reactive Plasmas (ICRP-10)
  • Int'l Joint Research
• [Presentation] Differences of intracellular molecular mechanisms of cell death between plasmaactivated medium-treated glioblastoma cells and plasma-activated Ringer’s lactate solution-treated glioblastoma cells (2019)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Yuko Katsumata, Kenji Ishikawa, Hiroki Kondo, Hiroshi Hashizume, Shinya Toyokuni, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori
  • Organizer: XXXIV International Conference on Phenomena in Ionized Gases (XXXIV ICPIG) and the 10th International Conference on Reactive Plasmas (ICRP-10)
  • Int'l Joint Research
• [Presentation] Optimized properties of carbon nanowall surface for laser desorption / ionization mass spectrometry (2019)
  • Author(s): Ryusei Sakai, Tomonori Ichikawa, Shun Imai, Hiroki Kondo, Kenji Ishikawa, Takayuki Ohta, Mineo Hiramatsu, Makoto Sekine, Masaru Hori
  • Organizer: XXXIV International Conference on Phenomena in Ionized Gases (XXXIV ICPIG) and the 10th International Conference on Reactive Plasmas (ICRP-10)
  • Int'l Joint Research
• [Presentation] Generation of bactericidal efficacy in neutral ph range using oxygen-radical treatment of organic compound (2019)
  • Author(s): Naoyuki Iwata, Vladislav Gamaleeve, Jun-Seok Oh, Hiroshi Hashizume, Takayuki Ohta, Kenji Ishikawa, Masaru Hori, Masafumi Ito
  • Organizer: XXXIV International Conference on Phenomena in Ionized Gases (XXXIV ICPIG) and the 10th International Conference on Reactive Plasmas (ICRP-10)
  • Int'l Joint Research
• [Presentation] Cell response in treatment of plasma-activated Ringer’s acetate (2019)
  • Author(s): Yuki Suda, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Shinya Toyokuni, Fumitaka Kikkawa, Masaaki Mizuno, Masaru Hori
  • Organizer: XXXIV International Conference on Phenomena in Ionized Gases (XXXIV ICPIG) and the 10th International Conference on Reactive Plasmas (ICRP-10)
  • Int'l Joint Research
• [Presentation] Behavior of metastable Ar atom generated in microwave excited atmospheric pressure Ar gas plasma jet in open air (2019)
  • Author(s): Keigo Takeda, Fumiya Satake, Masaki Kataoka, Mineo Hiramatsu, Kenji Ishikawa, Masaru Hori
  • Organizer: XXXIV International Conference on Phenomena in Ionized Gases (XXXIV ICPIG) and the 10th International Conference on Reactive Plasmas (ICRP-10)
  • Int'l Joint Research
• [Presentation] Intracellular responses of coccomyxa sp. during culture in plasma-treated nutrient solution (2019)
  • Author(s): Takumi Kato, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: Asian-European International Conference on Plasma Surface Engineering (AEPSE)
  • Int'l Joint Research
• [Presentation] Intracellular response of HeLa cells treated by plasma-activated Ringer's lactate solution (2019)
  • Author(s): Shogo Maeda, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Makoto Sekine, Fumitaka Kikkawa, Masaaki Mizuno, and Masaru Hori
  • Organizer: Asian-European International Conference on Plasma Surface Engineering (AEPSE)
  • Int'l Joint Research
• [Presentation] Yield improvement of rice plants with cold plasma treatment in paddy field during cultivation (2019)
  • Author(s): Hiroshi Hashizume, Hidemi Kitano, Genki Yuasa, Satoe Tohno, Hiromasa Tanaka, Kenji Ishikawa, Shogo Matsumoto, Hitoshi Sakakibara, Susumu Nikawa, Masayoshi Maeshima, Masaaki Mizuno, and Masaru Hori
  • Organizer: Asian-European International Conference on Plasma Surface Engineering (AEPSE)
  • Int'l Joint Research
• [Presentation] Lifetime of bactericidal efficacy in oxygen-radical-activated phenylalanine solution (2019)
  • Author(s): Naoyuki Iwata, Gamaleev Vladislav, Jun-Seok Oh, Hiroshi Hashizume, Takayuki Ohta, Kenji Ishikawa, Masaru Hori, and Masafumi Ito
  • Organizer: Asian-European International Conference on Plasma Surface Engineering (AEPSE)
  • Int'l Joint Research
• [Presentation] Similarities and differences of biological networks between PAM- and PAL-treated glioblastoma cells (2019)
  • Author(s): Masaru Hori, Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, and Fumitaka Kikkawa
  • Organizer: Asian-European International Conference on Plasma Surface Engineering (AEPSE)
  • Int'l Joint Research
• [Presentation] Gene expression analyses revealed the differences of intracellular molecular mechanisms of PAM- and PAL-induced cell death (2019)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Yuko Katsumata, Kenji Ishikawa, Hiroki Kondo, Hiroshi Hashizume, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, Fumitaka Kikkawa, and Masaru Hori
  • Organizer: Asian-European International Conference on Plasma Surface Engineering (AEPSE)
  • Int'l Joint Research
• [Presentation] Chemical reaction analysis on surface of glucose-film using sum-frequency generation spectroscopy (2019)
  • Author(s): Yuta Yoshida, Ryoya Katuya, Takayuki Ohta, Kenji Ishikawa, and Masaru Hori
  • Organizer: Asian-European International Conference on Plasma Surface Engineering (AEPSE)
  • Int'l Joint Research
• [Presentation] Spectroscopic diagnostics of microwave excited atmospheric pressure ar plasma jet in open air (2019)
  • Author(s): Keigo Takeda, Mineo Hiramatsu, Kenji Ishikawa, and Masaru Hori
  • Organizer: 72nd Annual Gaseous Electronics Conference (GEC) (Texas, USA)
  • Int'l Joint Research
• [Presentation] Radical activated solutions with bactericidal and plant-growth effects (2019)
  • Author(s): Masafumi Ito, Naoyuki Iwata, Vladislav Gamaleev, Hiroshi Hashizume, Jun-Seok Oh, Takayuki Ohta, Kenji Ishikawa, and Masaru Hori
  • Organizer: 72nd Annual Gaseous Electronics Conference (GEC) (Texas, USA)
  • Int'l Joint Research
• [Presentation] Non-thermal plasma as a powerful tool to synthesize bio-effective materials in solutions (2019)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Hiroki Kondo, Hiroshi Hashizume, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, Fumitaka Kikkawa, and Masaru Hori
  • Organizer: Material Research Meeting (MRM) 2019 (Yokohama)
  • Int'l Joint Research
• [Presentation] Modification of lipid metabolism in Coccomyxa sp. treated by non-equilibrium atmosphere pressure plasma (2019)
  • Author(s): Takumi Kato, Hiroki Kondo, Takayoshi Tsutsumi, Kenji Ishikawa, Makoto Sekine, and Masaru Hori
  • Organizer: Material Research Meeting (MRM) 2019 (Yokohama)
  • Int'l Joint Research
• [Presentation] Growth Promotion Effect of Oxygen-Radical-Treated Phenylalanine Solutions (2019)
  • Author(s): Naoyuki Iwata, Vladislav Gamaleeve, Hiroshi Hashizume, Jun-Seok Oh, Takayuki Ohta, Kenji Ishikawa, Masaru Hori, and Masafumi Ito
  • Organizer: Material Research Meeting (MRM) 2019 (Yokohama)
  • Int'l Joint Research
• [Presentation] Low Temperature Plasma Surface Interactions Among Multiple Phase (2019)
  • Author(s): Kenji Ishikawa, Masaru Hori
  • Organizer: 5th Asian Workshop on Applied Plasma Science and Engineering, Kuala Lumpur, Malaysia
  • Int'l Joint Research / Invited
• [Presentation] Mechanism of Selective Killing of Glioblastoma to Astrocytes in PlasmaActivated Lactate (2019)
  • Author(s): Yugo Hosoi, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Masaaki Mizuno, Fumitaka Kikkawa and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Aqueous Reactions in Non-equilibrium Atmospheric Pressure Plasma-activated Ringer s Lactate Solutions (2019)
  • Author(s): Yang Liu, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Takyoshi Tsutsumi, Fumitaka Kikkawa, Kae Nakamura, Masaaki Mizuno, Shinya Toyokuni, Hiroki Kondo, and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Intracellular Reactive Oxygen Species Responses of HeLa Cells Treated with PlasmaActivated Organics (2019)
  • Author(s): Shogo Maeda, Yugo Hosoi, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Makoto Sekine and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Regulation of Cell Proliferation Changing Duty Ratio of an Electrical Stimulation on Carbon Nanowalls Scaffold (2019)
  • Author(s): Tomonori Ichikawa, Hiroki Kondo, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Kenji Ishikawa, Makoto Sekine and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Morphological Dynamics of Dying Cells Incubated in Plasma-Activated Medium (2019)
  • Author(s): Nanami Ito, Maho Yamada, Yugo Hosoi, Hiromasa Tanaka, Kenji Ishikawa and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Cytotoxicity of Plasma-Activated Glucose and Amino Acids (2019)
  • Author(s): Maho Yamada, Nanami Ito, Yugo Hosoi, Hiromasa Tanaka, Kenji Ishikawa and Masaru Hori
  • Organizer: ISPlasma2019/IC-PLANTS2019, Nagoya Institute of Technology, Nagoya, Japan
  • Int'l Joint Research
• [Presentation] Intracellular responses in apoptotic cells to reactive species in plasma treated liquids (2018)
  • Author(s): K. Ishikawa, H. Tanaka, T. Kondo, S. Toyokuni, F. Kikkawa, M. Mizuno, and M. Hori
  • Organizer: 7th International Conference on Plamsa Medicine (ICPM7), Drexekl University Philadelphia, USA
  • Int'l Joint Research / Invited
• [Presentation] Low temperature plasma surface interactions for their future basic researches and applications (2018)
  • Author(s): K. Ishikawa, M. Itou, and M. Hori
  • Organizer: 22nd International Conference Gas Discharges and Their Applications, Serbia
  • Int'l Joint Research / Invited
• [Presentation] Mechanisms of plasma jets impinging upon liquids (2018)
  • Author(s): T. Brubaker, K. Ishikawa, H. Hashizume, H. Tanaka, T. Tsutsumi, H. Kondo, S. Knecht, S. Bilen, and M. Hori
  • Organizer: 7th International Conference on Plamsa Medicine (ICPM7), Drexekl University Philadelphia, USA
  • Int'l Joint Research
• [Presentation] Similarities and Differences in Gene Transcription in Plasma-activated Medium Treated and Plasma-activated Ringer’s Lactate Solution Treated Glioblastomas (2018)
  • Author(s): H. Tanaka, M. Mizuno, K. Ishikawa, K. Takeda, H. Hashizume, H. Kondo, M. Sekine, K. Nakamura, H. Kajiyama, S. Akiyama, S. Maruyama, S. Toyokuni, F. Kikkawa, M. Hori
  • Organizer: 7th International Conference on Plamsa Medicine (ICPM7), Drexekl University Philadelphia, USA
  • Int'l Joint Research
• [Presentation] Biological Effects of Plasma-Irradiated Oraganic Molecules in Plasma-Treated Liquids (2018)
  • Author(s): Kenji Ishikawa, Y. Hosoi, D.Kanno, Y. Kurokawa, H. Tanaka, M. Mizuno, F.Kikkawa, M.Hori
  • Organizer: AVS 65th INTERNATIONAL SYMPOSIUM & EXHIBITION 2018, Long Beach, California, USA
  • Int'l Joint Research
• [Presentation] Nonequilibrium atmospheric pressure plasma activates organic substances for antitumor effects (2018)
  • Author(s): Kenji Ishikawa, Yugo Hosoi, Hiromasa Tanaka, Hiroshi Hashizume, Shinya Toyokuni, Fumitaka Kikkawa, Masaaki Mizuno, and Masaru Hori
  • Organizer: 40th International Symposium on Dry Process (DPS2018), Toyoda Auditorium, Nagoya University, Nagoya, Aichi, Japan
  • Int'l Joint Research
• [Presentation] Anti-proliferative effect of glyoxylate generated in plasma-activated lactate solution (2018)
  • Author(s): Y. Hosoi, K. Ishikawa, H. Tanaka, H. Hashizume, F. Kikkawa, M. Mizuno and M. Hori
  • Organizer: 28th Materials Research Society of Japan (MRS-J) , Kitakyusyu International Conference Center, Fukuoka, Japan
  • Int'l Joint Research
• [Presentation] Cell response mechanisms of HeLa cells cultured with plasma-activated lactate Ringer’s solution (PAL) (2018)
  • Author(s): S. Maeda, Y. Hosoi, K. Ishikawa, H. Hashizume, H. Tanaka, T. Takayoshi, H. Kondo, M. Sekine, and M.Hori
  • Organizer: 28th Materials Research Society of Japan (MRS-J) , Kitakyusyu International Conference Center, Fukuoka, Japan
  • Int'l Joint Research
• [Presentation] Plasma-activated medium and plasma-activated Ringer’s lactate solution exhibited anti-tumor effects through different mechanisms (2018)
  • Author(s): Yugo Hosoi, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: ISPLasma - 10th International Symposium on Advanced Plasma Science and its Applications for Nitrides and Nanomaterials March 04-08, 2018
  • Int'l Joint Research
• [Presentation] Time evolution of reactive oxygen and nitrogen species in plasma-activated media (2018)
  • Author(s): Timothy Brubaker, Kenji Ishikawa, Keigo Takeda, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: ISPLasma - 10th International Symposium on Advanced Plasma Science and its Applications for Nitrides and Nanomaterials March 04-08, 2018
  • Int'l Joint Research
• [Presentation] Inhomogeneity of nitrite anion concentrations in depth and time by convection during and after plasma-liquid interactions (2018)
  • Author(s): Timothy R. Brubaker, Kenji Ishikawa, Keigo Takeda, Jun-Seok Oh, Masafumi Ito, Hiroki Kondo, Hiroshi Hashizume, Hiromasa Tanaka, Sean D. Knecht, Sven G. Bil?n, and Masaru Hori
  • Organizer: 2nd International Workshop on Plasma Agriculture, March 9-12, 2018, Takayama, Japan
  • Int'l Joint Research
• [Presentation] プラズマ活性乳酸の物理化学解析 (2018)
  • Author(s): 細井 祐吾, 石川 健治, 橋爪 博司, 田中 宏昌, 吉川 史隆, 水野 正明, 堀 勝
  • Organizer: 第65回 応用物理学会 春季学術講演会
• [Presentation] プラズマ活性ラクテックの抗腫瘍効果物質の解明 (2018)
  • Author(s): 細井 祐吾, 石川 健治, 橋爪 博司, 田中 宏昌, 吉川 史隆, 水野 正明, 堀 勝
  • Organizer: 第65回 応用物理学会 春季学術講演会
• [Presentation] プラズマ活性乳酸処理グリオーマのメタボロミクス解析 (2018)
  • Author(s): 細井 祐吾, 石川 健治, 田中 宏昌, 橋爪 博司, 豊國 伸哉, 吉川 史隆, 水野 正明, 堀 勝
  • Organizer: 第65回 応用物理学会 春季学術講演会
• [Presentation] プラズマ活性ラクテック（PAL）で培養処理によるしたヒーラ(HeLa)細胞応答メカニズム (2018)
  • Author(s): 前田 昌吾, 細井 祐吾, 石川 健治, 橋爪 博司, 田中 宏昌, 堀 勝
  • Organizer: 第65回 応用物理学会 春季学術講演会
• [Presentation] プラズマ活性ラクテック中脂質二重膜形状の高速原子間力顕微鏡観察 (2018)
  • Author(s): 山岡 壮太郎, 戸波 卓也, 橋爪 博司, 近藤 博基, 石川 健治, 田中 宏昌, 堀 勝
  • Organizer: 第65回 応用物理学会 春季学術講演会
• [Presentation] AC励起非平衡大気圧Arプラズマ生成活性種の時空間分布計測 (2018)
  • Author(s): 倉増 廉, 竹田 圭吾, 石川 健治, 堤 隆嘉, 橋爪 博司, 田中 宏昌, 近藤 博基, 関根 誠, 堀 勝
  • Organizer: 第65回 応用物理学会 春季学術講演会
• [Presentation] プラズマ活性培養液処理した細胞内酸化窒素活性種(RONS)の解析 (2017)
  • Author(s): 石川 健治, 田中 宏昌, 橋爪 博司, 竹田 圭吾, 太田 貴之, 伊藤 昌文, 中村 香江, 梶山 広明, 吉川 史隆, 豊國 伸哉, 水野 正明, 堀 勝
  • Organizer: 第７０回日本酸化ストレス学会学術集会 於：つくば国際会議場
• [Presentation] Programmed process for synthesis of calcium oxalate crystals in a bufferred glucose solution by irradiation with non-equilibrium atmospheric-pressure plasma (2017)
  • Author(s): Kenji Ishikawa, Naoyuki Kurake, Hiromasa Tanaka, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Yoko Yamanishi, Yuzuru Ikehara, and Masaru Hori
  • Organizer: iPlasmaNano-VIII July 2-6, 2017. the University of Antwerp, Antwerp, Belgium.
  • Int'l Joint Research
• [Presentation] Observation of intracellular-molecular changes in plasma-irradiated cells using multiplex coherent anti-Stokes Raman scattering microscopy (2017)
  • Author(s): Takayuki Ohta, Ryo Furuta, Naoyuki Kurake, Kenji Ishikawa, Keigo Takeda, Hiroshi Hashizume, Hiroki Kondo, Masafumi Ito, and Masaru Hori
  • Organizer: AEPSE 2017, Jeju International Convention Center, Jeju Island, Republic of Korea, September 11-15, 2017
  • Int'l Joint Research
• [Presentation] Ambient control during preparations of the plasma-activated medium (PAM) for antitumor effects (2017)
  • Author(s): Yugo Hosoi, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: AEPSE 2017, Jeju International Convention Center, Jeju Island, Republic of Korea, September 11-15, 2017
  • Int'l Joint Research
• [Presentation] Measurements of reactive species and VUV radiators generated by AC excited atmospheric pressure Ar plasma jet in open air (2017)
  • Author(s): Keigo Takeda, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: AEPSE 2017, Jeju International Convention Center, Jeju Island, Republic of Korea, September 11-15, 2017
  • Int'l Joint Research
• [Presentation] Analyses of cellular responses to Plasma-activated medium (PAM) and plasma-activated Ringer’s lactate solution (PAL) (2017)
  • Author(s): Hiromasa Tanaka, Masaru Hori
  • Organizer: AEPSE 2017, Jeju International Convention Center, Jeju Island, Republic of Korea, September 11-15, 2017
  • Int'l Joint Research
• [Presentation] 非平衡大気圧プラズマ源の構造検討 (2017)
  • Author(s): 勝野 楓、石川 健治、堤 隆嘉、竹田 圭吾、橋爪 博司、田中 宏昌、近藤 博基、関根 誠、堀 勝
  • Organizer: 第78回応用物理学会秋季学術講演会 於：福岡国際会議場
• [Presentation] プラズマ照射有機溶液を用いたシュウ酸カルシウム結晶の生成機構 (2017)
  • Author(s): 岡部 萌、倉家 尚之、石川 健治、田中 宏昌、橋爪 博司、堤 隆嘉、近藤 博基、関根 誠、堀 勝
  • Organizer: 第78回応用物理学会秋季学術講演会 於：福岡国際会議場
• [Presentation] カーボンナノウォールを用いた表面支援レーザー脱離/イオン化質量分析法によるペプチドの測定 (2017)
  • Author(s): 伊藤 寛納、太田 貴之、石川 健治、近藤 博基、平松 美根男、堀 勝
  • Organizer: 第78回応用物理学会秋季学術講演会 於：福岡国際会議場
• [Presentation] Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Observations of HeLa Cells Cultured in Non-equilibrium Atmospheric Pressure-Plasma-Activated Medium (PAM) (2017)
  • Author(s): Kenji Ishikawa, Ryo Furuta, Keigo Takeda, Takayuki Ohta, Masafumi Ito, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, Masaru Hori
  • Organizer: AVS - 4th International Symposium and Exhibition, October 29-November 03, 2017. Tampa, Florida, USA
  • Int'l Joint Research
• [Presentation] 未来の健康長寿社会を実現するプラズマ医療技術への挑戦 (2017)
  • Author(s): 石川健治，田中宏昌，豊國伸哉, 水野正明，吉川史隆，堀勝
  • Organizer: プラズマカンファレンス 於：姫路
  • Int'l Joint Research / Invited
• [Presentation] プラズマ活性ラクテック （ＰＡＬ）内成分の物理化学解析と抗腫瘍効果の解明 (2017)
  • Author(s): 細井 祐吾、石川 健治、田中 宏昌、橋爪 博司、水野 正明、堀 勝
  • Organizer: プラズマカンファレンス 於：姫路
  • Int'l Joint Research
• [Presentation] カーボンナノウォールを用いた表面支援レーザー脱離/イオン化質量分析法によるペプチドの分析 (2017)
  • Author(s): 伊藤 寛納、太田 貴之、石川 健治、近藤 博基、平松 美根男、堀 勝
  • Organizer: プラズマカンファレンス 於：姫路
  • Int'l Joint Research
• [Presentation] Intracellular responses during incubation in plasma-activated cell culture medium (PAM) (2017)
  • Author(s): Kenji Ishikawa, Ryo Furuta, Naoyuki Kurake, Keigo Takeda, Hiroshi Hashizume, Hiromasa Tanaka, Takayuki Ohta, Masafumi Ito, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: 27th annual meeting of MRS-J, Yokohama, Japan, December 5-7, 2017
  • Int'l Joint Research
• [Presentation] Dynamics of Intracellular Responses in HeLa Cells Cultured in Non-equilibrium Atmospheric Pressure-Plasma-treated Medium (PAM) (2017)
  • Author(s): Kenji Ishikawa
  • Organizer: APSPT-10 - 10th Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology, December 15-17, 2017. Taiwan.
  • Int'l Joint Research / Invited
• [Presentation] Quantitative pH imaging of plasma‐treated liquids using bromothymol blue (2017)
  • Author(s): Timothy Ryan Brubaker, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Masaru Hori
  • Organizer: APSPT-10 - 10th Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology, December 15-17, 2017. Taiwan.
  • Int'l Joint Research
• [Presentation] Effects of dielectric walls on atmospheric-pressure plasma discharges (2017)
  • Author(s): Kaede Katsuno, Kenji Ishikawa, Takayoshi Tsutsumi, Keigo Takeda, Hiroshi Hashizume, Hiromasa Tanaka, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: APSPT-10 - 10th Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology, December 15-17, 2017. Taiwan.
  • Int'l Joint Research
• [Presentation] Investigation the effects of gas flow rate on atmospheric pressure plasma (2017)
  • Author(s): Yu-Chun Lin, Kenji Ishikawa, Masaru Hori, and Meng-Jiy Wang
  • Organizer: APSPT-10 - 10th Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology, December 15-17, 2017. Taiwan.
  • Int'l Joint Research
• [Book] Chapter 2 Physical and Chemical Basis of Nonthermal Plasma, In "Plasma Medical Science" (2018)
  • Author(s): Kenji Ishikawa et al.
  • Total Pages: 103
  • Publisher: Academic Press
  • ISBN: 9780128150047
• [Remarks] ホームページ
  • URL: http://www.plasma.engg.nagoya-u.ac.jp/ishikawa/index.php/Home
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