|Budget Amount *help
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
|Outline of Final Research Achievements
Aiming to reproduce the function of phospholipid scramblases with artificial peptides, we searched for the optimal transmembrane amino acid sequence to scramble membrane phospholipids and used the peptides to realize a new method to control cell function and survival. We introduced polar amino acid residue(s) into the center of the transmembrane sequence of the peptides and optimized their phospholipid scramblase activity by changing the type and arrangement of the residues. By inserting the peptide with scramblase activity into the cell membranes, we succeeded in exposing the phosphatidylserine (PS) on the cytoplasmic side of the cell membrane to the outside, and in phagocytosis of the PS-exposed cells by macrophages.