|Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
|Outline of Final Research Achievements
Xenorhodopsin (XeR) is a light-driven inward proton pump that was discovered by Inoue et al. In this research project, we have obtained new insights about the isomerization process of retinal and the proton transfer process of XeR by spectroscopic measurements and amino acid modification experiments. In addition, by mutating a proline near the retinal, we obtained a long-wavelength shifted mutant of XeR, which is expected to be useful as an optogenetic tool.
On the other hand, we identified a second light-deriven inward proton pump, schizorhodopsin (SzR), which is different from XeR, from Asgardian archaea, and clarified its photoreaction process and three-dimensional structure. The similarity of the mechanism suggests that SzR and XeR realize the same function by convergent evolution at the molecular level.