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The study on the transport mechanism of light-driven inward proton pump and its application to optogenetics

Research Project

Project/Area Number 17H03007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical chemistry
Research InstitutionThe University of Tokyo (2018-2020)
Nagoya Institute of Technology (2017)

Principal Investigator

Inoue Keiichi  東京大学, 物性研究所, 准教授 (90467001)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Keywords光駆動内向きプロトンポンプロドプシン / ゼノロドプシン / シゾロドプシン / レチナール / 光反応サイクル / プロトン移動 / 過渡吸収分光 / 赤外分光 / ロドプシン / 内向きプロトンポンプ / オプトジェネティクス / レーザー分光 / プロトンポンプ
Outline of Final Research Achievements

Xenorhodopsin (XeR) is a light-driven inward proton pump that was discovered by Inoue et al. In this research project, we have obtained new insights about the isomerization process of retinal and the proton transfer process of XeR by spectroscopic measurements and amino acid modification experiments. In addition, by mutating a proline near the retinal, we obtained a long-wavelength shifted mutant of XeR, which is expected to be useful as an optogenetic tool.
On the other hand, we identified a second light-deriven inward proton pump, schizorhodopsin (SzR), which is different from XeR, from Asgardian archaea, and clarified its photoreaction process and three-dimensional structure. The similarity of the mechanism suggests that SzR and XeR realize the same function by convergent evolution at the molecular level.

Academic Significance and Societal Importance of the Research Achievements

今回内向きプロトンポンプ機能には、①単一のレチナールシッフ塩基のカウンターイオン、②親水的な細胞質側環境と脱プロトン化したプロトン受容基、③細胞外溶媒からレチナールへの直接のプロトン結合が重要であることが明らかとされ、構造が似通った外向きプロトンポンプとの機能の違いがなぜ達成されるかを明らかにすることができた。これらの知見は膜タンパク質のイオン輸送の方向がどの様に決まるかという問いに対し、新たな回答を与えるものである。また新たに作製したPoXeRの長波長吸収型改変体は、今後透過性が高く、細胞毒性の低い長波長光での光操作のためのオプトジェネティクスツール開発の基盤になると期待される。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report

Research Products

(151 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (10 results) Journal Article (37 results) (of which Int'l Joint Research: 20 results,  Peer Reviewed: 37 results,  Open Access: 18 results,  Acknowledgement Compliant: 1 results) Presentation (96 results) (of which Int'l Joint Research: 29 results,  Invited: 38 results) Book (1 results) Remarks (7 results)

  • [Int'l Joint Research] イスラエル工科大学(イスラエル)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] チェコ科学アカデミー(チェコ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] イスラエル工科大学(イスラエル)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] シエナ大学(イタリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] チェコ科学アカデミー(チェコ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ボーリング・グリーン州立大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Israel Institute of Technology(イスラエル)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Czech Academy of Sciences(チェコ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Siena(イタリア)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] TAT Rhodopsin Is an Ultraviolet-Dependent Environmental pH Sensor2021

    • Author(s)
      Kataoka Chihiro、Sugimoto Teppei、Shigemura Shunta、Katayama Kota、Tsunoda Satoshi P.、Inoue Keiichi、Beja Oded、Kandori Hideki
    • Journal Title

      Biochemistry

      Volume: 60 Pages: 899-907

    • DOI

      10.1021/acs.biochem.0c00951

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exploration of natural red-shifted rhodopsins using a machine learning-based Bayesian experimental design2021

    • Author(s)
      Inoue Keiichi、Karasuyama Masayuki、Nakamura Ryoko、Konno Masae、Yamada Daichi、Mannen Kentaro、Nagata Takashi、Inatsu Yu、Yawo Hiromu、Yura Kei、Beja Oded、Kandori Hideki、Takeuchi Ichiro
    • Journal Title

      Communications Biology

      Volume: 4 Pages: 362-362

    • DOI

      10.1038/s42003-021-01878-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin2021

    • Author(s)
      Oda Kazumasa、Nomura Takashi、Nakane Takanori、Yamashita Keitaro、Inoue Keiichi、Ito Shota、Vierock Johannes、Hirata Kunio、Maturana Andres D、Katayama Kota、( 32 authers )、Kandori Hideki、Hegemann Peter、Iwata So、Kubo Minoru、Nishizawa Tomohiro、Nureki Osamu
    • Journal Title

      eLife

      Volume: 10 Pages: 1-21

    • DOI

      10.7554/elife.62389

    • NAID

      120006996980

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crystal Structure of Schizorhodopsin Reveals Mechanism of Inward Proton Pumping2021

    • Author(s)
      Akimitsu Higuchi, Wataru Shihoya, Masae Konno, Tatsuya Ikuta, Hideki Kandori, Keiichi Inoue, Osamu Nureki
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 118 Pages: 1-11

    • DOI

      10.1073/pnas.2016328118

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gate-keeper of ion transport-a highly conserved helix-3 tryptophan in a channelrhodopsin chimera, C1C2/ChRWR2020

    • Author(s)
      Nagasaka Yujiro、Hososhima Shoko、Kubo Naoko、Nagata Takashi、Kandori Hideki、Inoue Keiichi、Yawo Hiromu
    • Journal Title

      Biophysics and Physicobiology

      Volume: 17 Pages: 59-70

    • DOI

      10.2142/biophysico.bsj-2020007

    • NAID

      130007879484

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Excitonic coupling effect on the circular dichroism spectrum of sodium-pumping rhodopsin KR22020

    • Author(s)
      Fujimoto Kazuhiro J.、Inoue Keiichi
    • Journal Title

      The Journal of Chemical Physics

      Volume: 153 Pages: 045101-045101

    • DOI

      10.1063/5.0013642

    • NAID

      120006948923

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Active Learning of Bayesian Linear Models with High-Dimensional Binary Features by Parameter Confidence-Region Estimation2020

    • Author(s)
      Inatsu Yu、Karasuyama Masayuki、Inoue Keiichi、Kandori Hideki、Takeuchi Ichiro
    • Journal Title

      Neural Computation

      Volume: 32 Pages: 1998-2031

    • DOI

      10.1162/neco_a_01310

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Active Learning for Level Set Estimation Under Input Uncertainty and Its Extensions2020

    • Author(s)
      Yu Inatsu, Masayuki Karasuyama, Keiichi Inoue, Ichiro Takeuchi
    • Journal Title

      Neural Computation

      Volume: 32 Pages: 2486-2531

    • DOI

      10.1162/neco_a_01332

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shining light on rhodopsin selectivity: How do proteins decide whether to transport H+ or Cl-?2020

    • Author(s)
      Keiichi Inoue
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 295 Pages: 14805-14806

    • DOI

      10.1074/jbc.h120.016032

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of microbial rhodopsin-like genes in Guillardia theta2020

    • Author(s)
      Konno Masae、Yamauchi Yumeka、Inoue Keiichi、Kandori Hideki
    • Journal Title

      PLOS ONE

      Volume: 15

    • DOI

      10.1371/journal.pone.0243387

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crystal structure of heliorhodopsin2020

    • Author(s)
      W. Shihoya, K. Inoue, M. Singh, M. Konno, S. Hososhima, K. Yamashita, K. Ikeda, A. Higuchi, T. Izume, Okazaki S, Hashimoto M, Mizutori R, Tomida S, Yamauchi Y, Abe-Yoshizumi R, K. Katayama, SP. Tsunoda, M. Shibata, Y. Furutani, A. Pushkarev, O. Béjà, T. Uchihashi, H. Kandori, O. Nureki
    • Journal Title

      Nature

      Volume: 574 Pages: 132-136

    • DOI

      10.1038/s41586-019-1604-6

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Allosteric Communication with the Retinal Chromophore upon Ion Binding in a Light-Driven Sodium Ion-Pumping Rhodopsin.2020

    • Author(s)
      A. Otomo, M. Mizuno, K. Inoue, H. Kandori, Y. Mizutani
    • Journal Title

      Biochemistry

      Volume: 59 (4) Pages: 520-529

    • DOI

      10.1021/acs.biochem.9b01062

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Infrared spectroscopic analysis on structural changes around the protonated Schiff base upon retinal isomerization in light-driven sodium pump KR2.2020

    • Author(s)
      S. Tomida, S. Ito, T. Mato, Y. Furutani, K. Inoue, H. Kandori
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Bioenergetics

      Volume: - Pages: 148190-148190

    • DOI

      10.1016/j.bbabio.2020.148190

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Schizorhodopsins: A novel family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps2020

    • Author(s)
      Keiichi Inoue, Satoshi P. Tsunoda, Manish Singh, Sahoko Tomida, Shoko Hososhima, Masae Konno, Ryoko Nakamura, Hiroki Watanabe, Paul-Adrian Bulzu, Horia L. Banciu, Adrian Andrei, Takayuki Uchihashi, Rohit Ghai, Oded Beja, Hideki Kandori
    • Journal Title

      Science Advances

      Volume: 6

    • DOI

      10.1126/sciadv.aaz2441

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Red-shiftingmutation of light-driven sodium-pump rhodopsin2019

    • Author(s)
      K. Inoue, M. Del Carmen Marin, S. Tomida, R. Nakamura, Y. Nakajima, M. Olivucci, H. Kandori*
    • Journal Title

      Nature Communications

      Volume: 10 Pages: 1993-1993

    • DOI

      10.1038/s41467-019-10000-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Engineered Functional Recovery of Microbial Rhodopsin without Retinal-Binding Lysine.2019

    • Author(s)
      Yumeka Yamauchi, Masae Konno, Daichi Yamada, Kei Yura, Keiichi Inoue, Oded Beja, Hideki Kandori
    • Journal Title

      Scientific Reports

      Volume: 印刷中 Pages: 1116-1121

    • DOI

      10.1111/php.13114

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Unique photochemistry observed in a new microbial rhodopsin2019

    • Author(s)
      C. Kataoka, K. Inoue, K. Katayama, O. Beja, H. Kandori*
    • Journal Title

      Journal of Physical Chemistry Letters

      Volume: 10 Pages: 5117-5121

    • DOI

      10.1021/acs.jpclett.9b01957

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Casting light on Asgardarchaeota metabolism in a sunlit microoxic niche2019

    • Author(s)
      Paul-Adrian Bulzu†, Adrian-Stefan Andrei†, Michaela M. Salcher, Maliheh Mehrshad, Keiichi Inoue, Hideki Kandori, Oded Beja;, Rohit Ghai*, Horia L. Banciu
    • Journal Title

      Nat. Microbiol.

      Volume: in press Pages: 1129-1137

    • DOI

      10.1038/s41564-019-0404-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ultrafast Dynamics of Heliorhodopsins2019

    • Author(s)
      Tahara Shinya、Singh Manish、Kuramochi Hikaru、Shihoya Wataru、Inoue Keiichi、Nureki Osamu、B?j? Oded、Mizutani Yasuhisa、Kandori Hideki、Tahara Tahei
    • Journal Title

      J. Phys. Chem. B.

      Volume: 123 Pages: 2507-2512

    • DOI

      10.1021/acs.jpcb.9b00887

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Heliorhodopsins are absent in diderm (Gram-negative) bacteria: Some thoughts and possible implications for activity2019

    • Author(s)
      Flores-Uribe Jos?、Hevroni Gur、Ghai Rohit、Pushkarev Alina、Inoue Keiichi、Kandori Hideki、B?j? Oded
    • Journal Title

      Environmental Microbiology Reports

      Volume: 印刷中

    • DOI

      10.1111/1758-2229.12730

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Resonance Raman Investigation of the Chromophore Structure of Heliorhodopsins2018

    • Author(s)
      Otomo Akihiro、Mizuno Misao、Singh Manish、Shihoya Wataru、Inoue Keiichi、Nureki Osamu、B?j? Oded、Kandori Hideki、Mizutani Yasuhisa
    • Journal Title

      J. Phys. Chem. Lett.

      Volume: 9 Pages: 6431-6436

    • DOI

      10.1021/acs.jpclett.8b02741

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Understanding Colour Tuning Rules and Predicting Absorption Wavelengths of Microbial Rhodopsins by Data-Driven Machine-Learning Approach2018

    • Author(s)
      Karasuyama Masayuki、Inoue Keiichi、Nakamura Ryoko、Kandori Hideki、Takeuchi Ichiro
    • Journal Title

      Scientific Reports

      Volume: 8 Pages: 15580-15580

    • DOI

      10.1038/s41598-018-33984-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structure of the natural anion-conducting channelrhodopsin GtACR12018

    • Author(s)
      Kim Yoon Seok、Kato Hideaki E.、Yamashita Keitaro、Ito Shota、Inoue Keiichi、Ramakrishnan Charu、Fenno Lief E.、Evans Kathryn E.、Paggi Joseph M.、Dror Ron O.、Kandori Hideki、Kobilka Brian K.、Deisseroth Karl
    • Journal Title

      Nature

      Volume: 561 Pages: 343-348

    • DOI

      10.1038/s41586-018-0511-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural mechanisms of selectivity and gating in anion channelrhodopsins2018

    • Author(s)
      Kato Hideaki E.、Kim Yoon Seok、Paggi Joseph M.、Evans Kathryn E.、Allen William E.、Richardson Claire、Inoue Keiichi、Ito Shota、Ramakrishnan Charu、Fenno Lief E.、Yamashita Keitaro、Hilger Daniel、Lee Soo Yeun、Berndt Andre、Shen Kang、Kandori Hideki、Dror Ron O.、Kobilka Brian K.、Deisseroth Karl
    • Journal Title

      Nature

      Volume: 561 Pages: 349-354

    • DOI

      10.1038/s41586-018-0504-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mutation Study of Heliorhodopsin 48C122018

    • Author(s)
      Singh Manish、Inoue Keiichi、Pushkarev Alina、Beja Oded、Kandori Hideki
    • Journal Title

      Biochemistry

      Volume: 57 Pages: 5041-5049

    • DOI

      10.1021/acs.biochem.8b00637

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Time-resolved FTIR study of light-driven sodium pump rhodopsins2018

    • Author(s)
      Chen Hui-Fen、Inoue Keiichi、Ono Hikaru、Abe-Yoshizumi Rei、Wada Akimori、Kandori Hideki
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Pages: 17694-17704

    • DOI

      10.1039/c8cp02599a

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A distinct abundant group of microbial rhodopsins discovered using functional metagenomics.2018

    • Author(s)
      A.Pushkarev, K. Inoue, S. Larom, J. Flores-Uribe, M. Singh, M. Konno, S. Tomida, S. Ito, R. Nakamura, S. P. Tsunoda, A. Philosof, I. Sharon, N. Yutin, E. V. Koonin, H. Kandori, O. Béjà
    • Journal Title

      Nature

      Volume: 558 Pages: 595-599

    • DOI

      10.1038/s41586-018-0225-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] "Light-driven sodium-pumping rhodopsin: a new concept of active transport"2018

    • Author(s)
      H. Kandori*, K. Inoue, S. P. Tsunoda
    • Journal Title

      Chem Rev.

      Volume: in press Pages: 10646-10658

    • DOI

      10.1021/acs.chemrev.7b00548

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-distance perturbation on Schiff base-counterion interactions by His30 and the extracellular Na+-binding site in Krokinobacter rhodopsin 22018

    • Author(s)
      Arisu Shigeta, Shota Ito, Rina Kaneko, Sahoko Tomida, Keiichi Inoue, Hideki Kandori, Izuru Kawamura
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 20 Pages: 8450-8455

    • DOI

      10.1039/c8cp00626a

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of temperature and hydration level on purple membrane dynamics studied using broadband dielectric spectroscopy from sub-GHz to THz regions2018

    • Author(s)
      Naoki Yamamoto, Shota Ito, Masahiro Nakanishi, Eri Chatani, Keiichi Inoue, Hideki Kandori, Keisuke Tominaga
    • Journal Title

      Journal of Physical Chemistry B

      Volume: 122 Pages: 1367-1377

    • DOI

      10.1021/acs.jpcb.7b10077

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chimeric microbial rhodopsins for optical activation of Gs-proteins2017

    • Author(s)
      K. Yoshida
    • Journal Title

      BIOPHYSICS

      Volume: 14 Issue: 0 Pages: 183-190

    • DOI

      10.2142/biophysico.14.0_183

    • NAID

      130006252951

    • ISSN
      2189-4779
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] "Unique hydrogen bonds in membrane protein monitored by whole mid-IR ATR spectroscopy in aqueous solution"2017

    • Author(s)
      S. Ito, M. Iwaki, S. Sugita, R. Abe-Yoshizumi, T. Iwata, K. Inoue, H. Kandori*
    • Journal Title

      J. Phys. Chem. B

      Volume: 122 Pages: 165-170

    • DOI

      10.1021/acs.jpcb.7b11064

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conversion of microbial rhodopsins: insights into functionally essential elements and rational protein engineering2017

    • Author(s)
      Kaneko Akimasa、Inoue Keiichi、Kojima Keiichi、Kandori Hideki、Sudo Yuki
    • Journal Title

      Biophys. Rev.

      Volume: 9 Pages: 861-876

    • DOI

      10.1007/s12551-017-0335-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] "Low-temperature FTIR spectroscopy provides evidence for protein-bound water molecules in eubacterial light-driven ion pumps "2017

    • Author(s)
      Y. Nomura, S. Ito, M. Teranishi, H. Ono, K. Inoue, H. Kandori*
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 20 Pages: 3165-3171

    • DOI

      10.1039/c7cp05674e

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular properties of a DTD channelrhodopsin from <i>Guillardia theta</i>2017

    • Author(s)
      Yamauchi Yumeka、Konno Masae、Ito Shota、Tsunoda Satoshi P.、Inoue Keiichi、Kandori Hideki
    • Journal Title

      BIOPHYSICS

      Volume: 14 Issue: 0 Pages: 57-66

    • DOI

      10.2142/biophysico.14.0_57

    • NAID

      130007040873

    • ISSN
      2189-4779
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] “Functional characterization of sodium-pumping rhodopsins with different pumping properties”2017

    • Author(s)
      S. P. Tsunoda, M. Prigge, R. Abe-Yoshizumi, K. Inoue, Y. Kozaki, T. Ishizuka, H. Yawo, O. Yizhar, H. Kandori*
    • Journal Title

      PLoS One.

      Volume: 12

    • DOI

      10.1371/journal.pone.0179232

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] FTIR analysis of a light-driven inward proton pumping rhodopsin at 77 K.2017

    • Author(s)
      Ito, S., Sugita, S., Inoue, K. and Kandori, H.
    • Journal Title

      Photochem. Photobiol.

      Volume: 印刷中 Pages: 1381-1387

    • DOI

      10.1111/php.12771

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] タンパク質のボトムアップ機能向上への機械学習法の応用2021

    • Author(s)
      井上 圭一
    • Organizer
      ISSPワークショップ「物性科学におけるデータ科学の今と未来」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Photobiology of new microbial rhodopsins2021

    • Author(s)
      Keiichi Inoue
    • Organizer
      BPS - Biophysical Society 65th Annual Meeting
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新奇な微生物型ロドプシンの光機能およびその光反応メカニズム2021

    • Author(s)
      井上 圭一
    • Organizer
      第3回晝間輝夫光科学賞・令和2年度研究助成金贈呈式
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 光駆動タンパク質ロドプシンの分子機能エンジニアリング2020

    • Author(s)
      井上 圭一
    • Organizer
      第72回日本生物工学会大会・シンポジウム「ボトムアップ生物工学」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 微生物ロドプシンの機能と色の制御2020

    • Author(s)
      井上 圭一
    • Organizer
      第58回日本生物物理学会年会・シンポジウム「Biomolecular Design to Control Their Functions」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Mutational analysis of the mechanism of an absorption red shift in a marine bacterial Cl--pumping rhodopsin2020

    • Author(s)
      永田 崇、烏山 昌幸、竹内 一郎、中島 悠、吉澤 晋、井上 圭一
    • Organizer
      第58回日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Development of a light regulatory system of membrane potential in plant cells2020

    • Author(s)
      今野 雅恵、八尾 寛、神取 秀樹、井上 圭一
    • Organizer
      第58回日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The gate-keeper role of a highly conserved helix-3 tryptophan for ion transport of the channelrhodopsin chimera, C1C2/ChRWR2020

    • Author(s)
      長坂 勇次郎、細島 頌子、神取 秀樹、井上 圭一、八尾 寛
    • Organizer
      第58回日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Biophysical characterization of different members of TAT rhodopsins: a new group of microbial rhodopsins2020

    • Author(s)
      萬年 健太郎、永田 崇、Oded Beja、井上 圭一
    • Organizer
      第58回日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Sir Martin Wood Prize Lecture: The emerging world of microbial rhodopsins in photobiology2020

    • Author(s)
      Keiichi Inoue
    • Organizer
      MPI-FKF Seminar
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sir Martin Wood Prize Lecture: The emerging world of microbial rhodopsins in photobiology2020

    • Author(s)
      Keiichi Inoue
    • Organizer
      MRC LMB Seminar
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光駆動型内向きプロトンポンプ、シゾロドプシンのプロトン輸送メカニズム2020

    • Author(s)
      井上 圭一、志甫谷 渉、今野 雅恵、樋口 晶光、但馬 聖也、古谷 祐詞、川﨑 佑真、永田 崇、八尾 寛、川村 出、神取 秀樹、濡木 理
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 光駆動型内向きH+ポンプ型シゾロドプシンのH+輸送メカニズムの分光及び構造研究2020

    • Author(s)
      井上 圭一、志甫谷 渉、今野 雅恵、樋口 晶光、但馬 聖也、古谷 祐詞、川﨑 佑真、永田 崇、八尾 寛、川村 出、神取 秀樹、濡木 理
    • Organizer
      日本生体エネルギー研究会第46回討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 海洋性細菌のCl-ポンプロドプシンにおける波長制御メカニズムの変異体解析2020

    • Author(s)
      永田 崇、烏山 昌幸、中島 悠、吉澤 晋、竹内 一郎、井上 圭一
    • Organizer
      日本生体エネルギー研究会第46回討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] TATロドプシンのpKaと多量体形成の制御における保存されたグルタミン酸の役割2020

    • Author(s)
      萬年 健太郎、永田 崇、戸叶 貴也、内橋 貴之、Oded Beja、井上 圭一
    • Organizer
      日本生体エネルギー研究会第46回討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 微生物ロドプシンの吸収波長の自在制御に向けた機械学習法の開発2020

    • Author(s)
      井上 圭一
    • Organizer
      日本バイオインダストリー協会・発酵と代謝研究会勉強会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] アスガルド古細菌の持つ新奇光駆動型内向きプロトンポンプ、シゾロドプシンの輸送メカニズム2020

    • Author(s)
      井上 圭一、志甫谷 渉、今野 雅恵、樋口 晶光、但馬 聖也、古谷 祐詞、川﨑 佑真、永田 崇、八尾 寛、川村 出、神取 秀樹、濡木 理
    • Organizer
      日本化学会 第101春季年会 (2021)
    • Related Report
      2020 Annual Research Report
  • [Presentation] Photobiology and Photochemistry of Microbial Rhodopsins2020

    • Author(s)
      井上圭一
    • Organizer
      2019年度ExCELLS若手リトリート
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] イオン輸送型ロドプシンのチャネル/ポンプ機能スイッチング2020

    • Author(s)
      長坂勇次郎、細島頌子、井上圭一、神取秀樹、八尾寛
    • Organizer
      第9回日本生物物理学会関東支部会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 新奇な微生物型ロドプシンの光機能およびその光反応メカニズム2020

    • Author(s)
      井上圭一
    • Organizer
      第2回晝間輝夫光科学賞・令和元年度研究助成金贈呈式
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ヘリオロドプシンの構造生物学的および物理化学的研究2020

    • Author(s)
      志甫谷渉、○井上圭一、Manish Singh、今野雅恵、細島頌子、山下恵太朗、池田健人、樋口昌光、岡崎早恵、井爪珠希、橋本真典、水鳥律、富田紗穂子、山内夢叶、吉住玲、片山耕大、角田聡、柴田幹大、古谷祐詞、Alina Pushkarev、Oded Beja、内橋貴之、神取秀樹、濡木理
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 新規生体分子ツール開発に向けた機械学習法の応用2019

    • Author(s)
      井上圭一
    • Organizer
      第16回AMO討論会「統計的機械学習が拓く新たな科学の地平線」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 新奇ロドプシンファミリーSchizorhodopsin (SzR)による光駆動内向きプロトン輸送2019

    • Author(s)
      井上圭一、角田聡、Manish Singh、今野雅恵、富田紗穂子、中村良子、渡辺大輝、内橋貴之、Rohit Ghai、Oded Beja、神取秀樹
    • Organizer
      第46回生体分子科学討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Schizorhodopsinの光駆動内向きプロトン輸送とその分子メカニズム2019

    • Author(s)
      井上圭一、角田聡、Manish Singh、今野雅恵、富田紗穂子、細島頌子、中村良子、渡辺大輝、内橋貴之、Rohit Ghai、Oded Beja、神取秀樹
    • Organizer
      ISSPワークショップ「レチナールタンパク質の光機能発現の物理と化学」
    • Related Report
      2019 Annual Research Report
  • [Presentation] 光受容型膜タンパク質微生物型ロドプシンの多様な機能メカニズムと内部結合水2019

    • Author(s)
      井上圭一
    • Organizer
      第1回新世代研究所(ATI)水和ナノ構造研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 光エネルギーで働く微生物型ロドプシンの新世界2019

    • Author(s)
      井上圭一
    • Organizer
      群馬大学理工学研究院大学院セミナー
    • Related Report
      2019 Annual Research Report
  • [Presentation] 微生物の持つ光機能性膜タンパク質ロドプシンの多様性とオプトジェネティクスの拡がり2019

    • Author(s)
      井上圭一
    • Organizer
      超スマート社会の構築に繋がる革新的材料創出に向けた光・量子ビーム応用技術調査専門委員会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ヘリオロドプシンとシゾロドプシン:2つの新奇光受容膜タンパク質ファミリーの光反応ダイナミクス2019

    • Author(s)
      井上圭一、Oded Beja、神取秀樹
    • Organizer
      第13回分子科学討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細菌や古細菌の持つ微生物型ロドプシンの光駆動プロトン輸送機構の多様性2019

    • Author(s)
      井上圭一
    • Organizer
      第92回生化学会大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 急速に拡大する微生物型ロドプシンワールド2019

    • Author(s)
      井上圭一
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 分子の構造とメカニズムから学ぶ、光受容膜タンパク質ロドプシンのデザイン2019

    • Author(s)
      井上圭一
    • Organizer
      筑波大プレ戦略研究会「生命から学ぶ」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 光受容膜タンパク質微生物型ロドプシンの新地平:ヘリオロドプシンの発見と展開2019

    • Author(s)
      井上圭一
    • Organizer
      日本生物物理学会北海道支部講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Spectroscopic and structural studies on new types of rhodopsins: Heliorhodopsin and schizorhodopsin2019

    • Author(s)
      Keiichi Inoue
    • Organizer
      Indo-Japan workshop Frontiers in Molecular Spectroscopy_From Fundamentals to Applications in Chemistry and Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 2つの新奇ロドプシン、ヘリオロドプシンとシゾロドプシンについて2019

    • Author(s)
      井上圭一、Oded Beja、神取秀樹
    • Organizer
      基生研研究会「異分野融合による次世代光生物学研究会」
    • Related Report
      2019 Annual Research Report
  • [Presentation] Discovery and functional analysis of novel retinal proteins2019

    • Author(s)
      Keiichi Inoue
    • Organizer
      Millennium Science Forum
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 研究室の立ち上げ秘話・大学編 ~東京大学物性研究所に着任して~2019

    • Author(s)
      井上圭一
    • Organizer
      日本生体エネルギー研究会 第45回討論会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 微生物型ロドプシンの光機能とその分子メカニズム2019

    • Author(s)
      井上圭一
    • Organizer
      第3回先端光機能計測研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] The transport mechanism of a new light-driven inward proton pump, schizorhodopsin (SzR)2019

    • Author(s)
      井上圭一、角田聡、Manish Singh、今野雅恵、富田紗穂子、中村良子、渡辺大輝、内橋貴之、Rohit Ghai、Oded Beja、神取秀樹
    • Organizer
      日本化学会第99春季年会2019
    • Related Report
      2018 Annual Research Report
  • [Presentation] 合成系から見た分子集合体の機能創成―生体材料2019

    • Author(s)
      井上圭一
    • Organizer
      日本化学会第99春季年会(2019)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 光受容型膜タンパク質・微生物型ロドプシンの物理化学研究2019

    • Author(s)
      井上圭一
    • Organizer
      2018年度「物質階層原理研究」・「ヘテロ界面研究」研究報告会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 微生物の持つロドプシンの多様な世界2019

    • Author(s)
      井上圭一
    • Organizer
      大阪市立大学セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 新奇光駆動型内向きプロトンポンプ型ロドプシンSchizorhodopsin (SzR)とその輸送メカニズムの分光研究2018

    • Author(s)
      井上圭一、角田聡、Manish Singh、今野雅恵、富田紗穂子、中村良子、渡辺大輝、内橋貴之、Rohit Ghai、Oded Beja、神取秀樹
    • Organizer
      日本生体エネルギー研究会 第44回討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 第3のロドプシン・ヘリオロドプシン2018

    • Author(s)
      井上圭一
    • Organizer
      第三回ルミノジェネティクス研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光エネルギーを使った微生物型ロドプシンの多様な機能とその分子メカニズム2018

    • Author(s)
      井上圭一
    • Organizer
      千葉大キラリティー研究センター定例研究会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Spectroscopic Study on the Functional Mechanism of New Microbial Rhodopsin Families2018

    • Author(s)
      Keiichi Inoue
    • Organizer
      India-Japan Mini-workshop
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biophysical Study on Heliorhodopsin2018

    • Author(s)
      Keiichi Inoue
    • Organizer
      18th International Conference on Retinal Proteins
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光受容膜タンパク質微生物型ロドプシンの多様性とその分子メカニズム2018

    • Author(s)
      井上圭一
    • Organizer
      東京工業大学化学科セミナー~物理化学研究の新潮流
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ゲノム科学により拡がる新奇微生物型ロドプシンの多様性と光化学2018

    • Author(s)
      井上圭一
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 「微生物型ロドプシン」と「深海・機械学習・聖書etc.」2018

    • Author(s)
      井上圭一
    • Organizer
      第8回生体物理化学セミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] Microbial Rhodopsin: Photo-receptive Membrane Proteins with Diverse Functions2018

    • Author(s)
      Keiichi Inoue
    • Organizer
      14th Japanese-German Frontiers (JGFoS) of Science Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] How the Light-driven Ion Pump Rhodopsins Decide What Ion Species They Transport?2018

    • Author(s)
      Keiichi Inoue
    • Organizer
      The 79th Okazaki Conference: Synthetic, Biological, and Hybrid Molecular Engines
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光受容型膜タンパク質微生物型ロドプシンの機能の多様性の起源に迫る2018

    • Author(s)
      井上圭一
    • Organizer
      物性研究所談話会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 新奇ヘリオロドプシンの発見と光反応過程の分光研究2018

    • Author(s)
      井上圭一
    • Organizer
      細胞構成研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Study on a New Distinct Family of Rhodopsin, Heliorhodopsin2018

    • Author(s)
      Keiichi Inoue, Alina Pushkarev, Manish Singh, Masae Konno, Sahoko Tomida, Shota Ito, Ryoko Nakamura, Satoshi P. Tsunoda, Oded Beja, Hideki Kandori
    • Organizer
      10th Optogenetics Research Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オプトジェネティクスに向けたナトリウムポンプ型ロドプシンの長波長化とそのメカニズム2018

    • Author(s)
      井上圭一、Maria del Carmen Marin、中村良子、中島悠太、Massimo Olivucci、神取秀樹
    • Organizer
      第45回生体分子科学討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The New Era of Marine Bacterial Photobiology- New Types of Microbial Rhodopsin2018

    • Author(s)
      Keiichi Inoue
    • Organizer
      3rd Global Congress & Expo on Mterials Science & Engineering (GCEMSE-2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 微生物型ロドプシンの光機能メカニズム研究2018

    • Author(s)
      井上圭一
    • Organizer
      LASORセミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] 新奇微生物型ロドプシン探索が拓く新たなオプトジェネティクス2018

    • Author(s)
      井上圭一
    • Organizer
      日本学術振興会光ネットワークシステム技術第171委員会・第63回研究会「生命ネットワーク・細胞操作と光技術」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 海洋性真核藻類がもつ光駆動カチオンャネルGtCCR4の光反応解析2018

    • Author(s)
      山内夢叶、今野雅恵、伊藤奨太、井上圭一、神取秀樹
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 長波長光駆動型ナトリウムポンプロドプシンの構築2018

    • Author(s)
      井上圭一、中村良子、神取秀樹
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Na+ポンプ型ロドプシンKR2のアミノ酸変異による波長制御2018

    • Author(s)
      中島悠太、中村良子、井上圭一、神取秀樹
    • Organizer
      平成29年度生物物理学会中部支部講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] KR2の効率的なナトリウム取り込みのために重要なQ123とS64周辺の水素結合ネットワーク2018

    • Author(s)
      富田紗穂子、伊藤奨太、井上圭一、神取秀樹
    • Organizer
      第5回将来を見据えた生体分子の構造・機能解析から分子設計に関する研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光駆動型内向きプロトンポンプロドプシンのH+輸送機構についての分光研究2018

    • Author(s)
      井上圭一、伊藤奨太、加藤善隆、野村祐梨香、柴田幹大、内橋貴之、角田聡、神取秀樹
    • Organizer
      第44回生体分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規微生物型ロドプシン研究とオプトジェネティックス2017

    • Author(s)
      井上圭一
    • Organizer
      日本学術振興会光電相互変換第125委員会・研究会「光技術が切り開く新しいバイオサイエンス・テクノロジー」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] イオンの輸送を決定するロドプシンタンパク質の柔らかさ2017

    • Author(s)
      井上圭一
    • Organizer
      第66回高分子討論会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 海の細菌のロドプシンが行う、光を使った多様なイオン輸送2017

    • Author(s)
      井上圭一
    • Organizer
      神戸大学先端融合科学シンポジウム「非共有結合系の分子科学:構造と機能」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 海の細菌のロドプシンが行う、光を使った多様なイオン輸送2017

    • Author(s)
      井上圭一
    • Organizer
      第二回ルミノジェネティクス研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Microbial rhodopsins: New types of machinery from marine bacteria2017

    • Author(s)
      K. Inoue
    • Organizer
      IMS Asian International Symposium “Japan-China Joint Interdisciplinary Symposium on Coordination-based Hybrid Materials”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Physicochemical study on the transport mechanism of novel types of microbial light-driven ion pump rhodopsins2017

    • Author(s)
      K. Inoue
    • Organizer
      International Symposium on Biophysics of Rhodopsins
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 海洋性細菌の持つ微生物型ロドプシンによる光を使ったイオン輸送の多様性とメカニズム2017

    • Author(s)
      井上圭一
    • Organizer
      機能物性セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Water-containing hydrogen bonding network in a light-driven Na+/H+ compatible pump KR22017

    • Author(s)
      S. Tomida, S. Ito, K. Inoue, H. Kandori
    • Organizer
      Gordon Research Conference on Photosensory Receptors and Signal Transduction
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Water-containing hydrogen bonding network in a light-driven Na+/H+ compatible pump KR22017

    • Author(s)
      S. Tomida, S. Ito, K. Inoue, H. Kandori
    • Organizer
      Gordon Research Seminar on Photosensory Receptors and Signal Transduction
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The important structural change of hydrogen bonding network in KR2 triggered by retinal isomerization for efficient Na+ uptake2017

    • Author(s)
      S. Tomida, S. Ito, K. Inoue, H. Kandori
    • Organizer
      2nd FRIMS International Symposium on Frontier Materials
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アミノ酸変異によるナトリウムポンプ型ロドプシンの長波長吸収化2017

    • Author(s)
      井上圭一、中村良子、神取秀樹
    • Organizer
      日本生体エネルギー研究会第43回討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 能動輸送のパナマ運河モデルと光駆動ナトリウムポンプの特殊性2017

    • Author(s)
      神取秀樹、井上圭一、角田 聡
    • Organizer
      日本生体エネルギー研究会第43回討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 海洋性真核藻類がもつ光駆動カチオンャネルGtCCR4の光反応中間体の解析2017

    • Author(s)
      山内夢叶、今野雅恵、伊藤奨太、井上圭一、神取秀樹
    • Organizer
      日本生体エネルギー研究会第43回討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新たに見つかった光駆動型外向きプロトンポンプロドプシンの機能解析と分光研究2017

    • Author(s)
      片岡千尋、井上圭一、神取秀樹
    • Organizer
      日本生体エネルギー研究会第43回討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The spectroscopic study on the proton transport mechanism of inward proton pump rhodopsin, PoXeR2017

    • Author(s)
      K. Inoue, H. Kandori
    • Organizer
      8th Asia and Oceania Conference on Photobiology (AOCP2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A light-driven chloride and sulfate pump from Bacillus aurantiacus2017

    • Author(s)
      M. Singh, S. Ito, S. Hososhima, R. Abe-Yoshizumi, S. P. Tsunoda, K. Inoue, H. Kandori
    • Organizer
      8th Asia and Oceania Conference on Photobiology (AOCP2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gene Expression Analysis of Microbial Rhodopsin-Like Genes in Marine Cryptomonad2017

    • Author(s)
      M. Konno, Y. Yamauchi, K. Inoue, H. Kandori
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光駆動カチオンチャネルGtCCR4を対象とする光反応過程の解析2017

    • Author(s)
      山内夢叶、今野雅恵、伊藤奨太、角田聡、井上圭一、神取秀樹
    • Organizer
      第7回CSJ化学フェスタ2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新たに見つかったH+ドナーを持たないロドプシンの機能解析と分光研究2017

    • Author(s)
      片岡千尋、井上圭一、神取秀樹
    • Organizer
      第7回CSJフェスタ 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] KR2の効率的なNa+輸送のためのモチーフアミノ酸と内部結合水の形成する水素結合環境2017

    • Author(s)
      富田紗穂子、伊藤奨太、井上圭一、神取秀樹
    • Organizer
      第7回CSJフェスタ 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光駆動型内向きプロトンポンプロドプシンのプロトン輸送メカニズムの分光研究2017

    • Author(s)
      井上圭一、伊藤奨太、加藤善隆、野村祐梨香、柴田幹大、内橋貴之、角田聡、神取秀樹
    • Organizer
      第11回分子科学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Red-shifted sodium pump rhodopsin variants for optogenetic application2017

    • Author(s)
      K. Inoue, R. Nakamura, H. Kandori
    • Organizer
      The 55th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Aromatic amino acids of a light-driven sodium pump KR2 are important to form an oligomer2017

    • Author(s)
      K. Inoue、H. Kandori
    • Organizer
      The 55th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional and Structural Analysis of NTQ type Anion Pump Rhodopsins2017

    • Author(s)
      K. Inoue、H. Kandori
    • Organizer
      The 55th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spectroscopic analysis of a light-gated cation channel GtCCR4 from marine algae2017

    • Author(s)
      K. Inoue、H. Kandori
    • Organizer
      The 55th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis and spectroscopic study of newly discovered light-driven outward proton pump DTS rhodopsins2017

    • Author(s)
      K. Inoue、H. Kandori
    • Organizer
      The 55th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structural Change of Water Molecules at Na+ Transport Pathway on KR22017

    • Author(s)
      S. Tomida, S. Ito, K. Inoue, H. Kandori
    • Organizer
      The 55th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海洋性クリプト藻Guillardia thetaが持つ微生物型ロドプシン様遺伝子の発現解析2017

    • Author(s)
      今野雅恵、井上圭一、神取秀樹
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Functional analysis and spectroscopic study of newly discovered light-driven outward proton pump rhodopsins2017

    • Author(s)
      C. Kataoka, K. Inoue, H. Kandori
    • Organizer
      Soft molecular systems, 5th symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of internal water molecules of a light-driven sodium pump KR22017

    • Author(s)
      S. Tomida, S. Ito, K. Inoue, H. Kandori
    • Organizer
      International Symposium on Biophysics oh Rhodopsins
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis and spectroscopic study of newly discovered light-driven outward H+ pump rhodopsins2017

    • Author(s)
      C. Kataoka, K. Inoue, H. Kandori
    • Organizer
      International Symposium on Biophysics of Rhodopsins
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] FTIR analysis of internal water molecules of a light-driven sodium pump KR22017

    • Author(s)
      S. Tomida, S. Ito, K. Inoue, H. Kandori
    • Organizer
      253rd American Chemical Society National Meeting & Exposition
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular properties of the light-gated cation channel from cryptophyta Guillardia theta2017

    • Author(s)
      Y. Yamauchi, M. Konno, S. Ito, Y. Kato, S. Tsunoda, K. Inoue, H. Kandori
    • Organizer
      253rd American Chemical Society National Meeting & Exposition
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Book] Optogenetics: Light-Sensing Proteins and Their Applications in Neuroscience and Beyond2021

    • Author(s)
      Keiichi Inoue
    • Total Pages
      37
    • Publisher
      Springer Nature
    • ISBN
      9789811587634
    • Related Report
      2020 Annual Research Report
  • [Remarks] 東京大学物性研究所・井上研究室

    • URL

      https://inoue.issp.u-tokyo.ac.jp/

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] アスガルドアーキアの持つ、光エネルギーを使って 水素イオンを取込むタンパク質の構造を解明

    • URL

      http://www.issp.u-tokyo.ac.jp/maincontents/news2.html?pid=12891

    • Related Report
      2020 Annual Research Report
  • [Remarks] 真核生物の祖先に最も近縁なアスガルド古細菌の持つ、 新しい光受容タンパク質の機能を解明

    • URL

      http://www.issp.u-tokyo.ac.jp/maincontents/news2.html?pid=10358

    • Related Report
      2019 Annual Research Report
  • [Remarks] 新型の光応答性タンパク質であるヘリオロドプシンの構造を解明

    • URL

      http://www.issp.u-tokyo.ac.jp/maincontents/news2.html?pid=8768

    • Related Report
      2019 Annual Research Report
  • [Remarks] 光でイオンを輸送するタンパク質、ロドプシンの吸収波長の長波長化に成功

    • URL

      http://www.issp.u-tokyo.ac.jp/maincontents/news2.html?pid=8101

    • Related Report
      2019 Annual Research Report
  • [Remarks] researchmap - 井上 圭一

    • URL

      https://researchmap.jp/k_inoue/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 東京大学・物性研究所・機能物性研究グループ・井上研究室ホームページ

    • URL

      http://inoue.issp.u-tokyo.ac.jp/

    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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