| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
A method of time-resolved rotational diffusion coefficient measurement was developed to investigate conformation change dynamics of proteins during reactions. For this purpose, luminescence from a metal complex that has a long lifetime was used. Some mutants of Lysozume were prepared and the probe molecule was bound to some sites of that protein. It was confirmed that the secondary structure does not change by the probe molecule. From the decay rate of the polarization of the luminescence, the rotational diffusion coefficient was determined as a function of the concentration of a denaturant. It is interesting to find that the rotational diffusion rate depends on the position of the probe molecule. This result indicates that the unfolding process should not be described by the two-state model, which has been reported by many researchers so far. A new stopped flow system was also developed to be used for the time-resolved rotational diffusion coefficient method.
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