| Project/Area Number |
17H03642
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Miki Kunio 京都大学, 理学研究科, 名誉教授 (10116105)
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| Co-Investigator(Kenkyū-buntansha) |
藤橋 雅宏 京都大学, 理学研究科, 助教 (10397581)
渡部 聡 東北大学, 多元物質科学研究所, 助教 (50432357)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | Ｘ線結晶解析 / ヒドロゲナーゼ / タンパク質の成熟化 / Hyp成熟化タンパク質 / 構造機能相関 / 構造生物学 / 成熟化タンパク質 / X線結晶解析 |
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| Outline of Final Research Achievements |
[NiFe] hydrogenases catalyze reversible hydrogen production/consumption. We have elucidated the molecular mechanism of maturation process of [NiFe] hydrogenases where the Ni/Fe cluster, an active center of the enzyme, is incorporated into protein precursors of hydrogenase in vivo. We have already determined crystal structures of six Hyp proteins which catalyze a series of maturation process of [NiFe] hydrogenases. We focused here on the structures of transient complexes including Hyp proteins which are formed during a series of reactions of maturation process. We succeeded in crystal structure determination including the complex between a nickel chaperon HypA and a large subunit precursor of hydrogenase. Consequently, we have acquired new important knowledge of the mechanism for Ni atom incorporation from the viewpoint of structural biology.
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| Academic Significance and Societal Importance of the Research Achievements |
[NiFe]ヒドロゲナーゼはプロトンから水素分子への可逆的な酸化還元反応を触媒する．多くの細菌におけるエネルギー代謝に関わっており，次世代クリーンエネルギーとの関連からも注目されている．その活性中心であるNi，Feの金属クラスターを生体内でヒドロゲナーゼ前駆体に組み込んで機能をもつ酵素にするHyp成熟化タンパク質群を対象に構造生物学的研究を行った．ここでは，Hypタンパク質の一つとヒドロゲナーゼ前駆体との過渡的複合体の結晶構造の決定に成功した．その結果，成熟化反応の過程をスナップショットして捉えることができ，[NiFe]ヒドロゲナーゼの生体内機能を発現させる分子機構に重要な知見が得られた．
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