| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
Rab11, which is a small GTPase, is localized in the Golgi apparatus and endosomes, and plays important roles such as endocytic recycling of cell membrane proteins and cell migration. Rab11 is also suggested to be associated with serious diseases such as ovarian cancer and breast cancer. In this study, we first clarified the molecular mechanism by which the Rab11 GDP / GTP exchange factor, REI-1 / SH3BP5 family, activates Rab11 by crystal structure analysis. In addition, we performed functional analysis of SH3BP5 and SH3BP5L in cultured mammalian cells and found that they localized to recycling endosomes and regulate cell migration by controlling intracellular trafficking of integrins.
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