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Analysis of lipid transfer mechanism and organelle interactions required for multifunction of lipids

Research Project

Project/Area Number 17H03692
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

Hajime Wada  東京大学, 大学院総合文化研究科, 教授 (60167202)

Co-Investigator(Kenkyū-buntansha) 三芳 秀人  京都大学, 農学研究科, 教授 (20190829)
安部 真人  愛媛大学, 農学研究科, 准教授 (30543425)
小林 康一  大阪府立大学, 高等教育推進機構, 准教授 (40587945)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywords脂質 / 脂質輸送タンパク質 / ホスファチジルコリン / コンタクトサイト / シロイヌナズナ / ゼニゴケ / 葉緑体 / 小胞体 / 合成脂質プローブ分子 / 脂質輸送メカニズム / 植物 / 生理学
Outline of Final Research Achievements

In this study, we have performed bioinformatic analysis and biochemical-molecular biological analysis with chemically synthesized lipid probes to identify proteins and organelle contact sites involved in lipid transfer in plant cells. We succeeded to detect many proteins, which bind to phosphatidylcholine (PC), in envelope membranes of chloroplasts isolated from spinach leaves. Some of the proteins might be involved in PC transfer from ER to chloroplasts. We also identified 7 and 3 proteins containing START domains in the Arabidopsis and liverwort genomes, respectively, as candidates for PC transfer proteins. One of the liverwort proteins is localized in the contact sites between chloroplasts and ER, and its mutant strain showed different lipid composition compared to that of wild-type strain, suggesting that it is involved in PC transfer from ER to chloroplasts.

Academic Significance and Societal Importance of the Research Achievements

植物細胞において脂質輸送に関わっているオルガネラ間のコンタクトサイトやLTPの実体は殆どわかっていない。本研究によって得られた成果は、脂質輸送における「オルガネラ間の相互作用と輸送メカニズム」を解き明かす端緒となるであろう。また、得られた知見に基づいて、将来的には脂質輸送システムを人為的に制御することが可能となり、細胞内に分配される各脂質分子の部位特異的な機能の解明に大きく貢献するものと考えられる。さらに、輸送システムを人為的に制御することにより、輸送の生理的意義の解明および多様なストレスに適応する植物の作出にも貢献することが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (7 results)

All 2020 2019 2018 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (6 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results)

  • [Journal Article] Plant and algal lipids set sail for new horizons2019

    • Author(s)
      Yonghua Li-Beisson and Hajime Wada
    • Journal Title

      Plant and Cell Physiology

      Volume: -

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ゼニゴケの葉緑体膜脂質代謝に関与する脂質輸送タンパク質の解析2020

    • Author(s)
      平嶋孝志、神保晴彦、小林康一、和田元
    • Organizer
      日本植物生理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] START ドメインをもつ脂質輸送タンパク質のゼニゴケにおける機能解析2019

    • Author(s)
      平嶋孝志、神保晴彦、小林康一、和田元
    • Organizer
      日本植物脂質シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] Roles of MGDG and DGDG in etioplasts of dark-grown Arabidopsis2018

    • Author(s)
      Koichi Kobayashi
    • Organizer
      International Symposium on Plant Lipids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Organic synthesis of phosphatidylglycerol analogues and their effects on the growth of pgA mutant of PCC 68032018

    • Author(s)
      Masato Abe
    • Organizer
      International Symposium on Plant Lipids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Crucial importance of chain length of fatty acids bound to phosphatidylglycerl for growth and photosynthesis2017

    • Author(s)
      Hajime Wada
    • Organizer
      The 7th Asian Symposium on Plant Lipids
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Distinct and overlapped roles of MGDG and DGDG in etioplasts of dark-grown Arabidopsis2017

    • Author(s)
      Koichi Kobayashi
    • Organizer
      The 7th Asian Symposium on Plant Lipids
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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