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Epigenetic signaling pathway between mitochondria and nucleus

Research Project

Project/Area Number 17H03749
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Science in genetics and breeding
Research InstitutionYokohama City University

Principal Investigator

Kinoshita Tetsu  横浜市立大学, 木原生物学研究所, 教授 (60342630)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywordsエピジェネティクス / ミトコンドリア / イネ / ATM3 / 鉄硫黄クラスター / Fe-Sクラスター / DNA脱メチル化 / DRE2 / 細胞質置換系統 / 胚乳
Outline of Final Research Achievements

It is well know that mitochondria and nucleus are interdependent. DNA demethylases in the nucleus require Fe-S clusters as cofactors. The biogenesis of this Fe-S cofactor starts from glutathione, which is exported through mitchondorial ATM3 transporter. We previously identified several Arabidopsis mutants related to this cytosolic Fe-S cluster biogenesis pathway, based on the phenotype that do not activate the imprinted FWA expression. In this study, we focus on the OsATM3 gene and generate its knock out plants by CRISPR/Cas9 system to understand role of ATM3. As a result, we found that phenotype of osatm2-/- is closely resembles to DNA demethylase mutant in the endosperm.

Academic Significance and Societal Importance of the Research Achievements

ミトコンドリアから核へのエピジェネティックなレトログレードシグナルには不明な点が多い。今回、核においてDNAメチル化の消去を行うDNA脱メチル化酵素の変異体と、DNA脱メチル化酵素に必要とされる補酵素の生合成の初発段階を担うミトコンドリア内膜のATM3トランスポーターの

Report

(5 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (2 results)

All 2019 2018

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] Optimized Method of Extracting Rice Chloroplast DNA for High-Quality Plastome Resequencing and de Novo Assembly2018

    • Author(s)
      Takamatsu Takeshi、Baslam Marouane、Inomata Takuya、Oikawa Kazusato、Itoh Kimiko、Ohnishi Takayuki、Kinoshita Tetsu、Mitsui Toshiaki
    • Journal Title

      Frontiers in Plant Science

      Volume: 9 Pages: 1-13

    • DOI

      10.3389/fpls.2018.00266

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] イネの鉄硫黄クラスター生合成経路遺伝子OsATM3の機能解析2019

    • Author(s)
      丸岡博、小野明美、永田博基、遠藤真咲、三上雅史、木下哲
    • Organizer
      日本育種学会第136回講演会
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2023-01-30  

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