• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism of important traits in Brassicaceae floricultural plant

Research Project

Project/Area Number 17H03765
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionShizuoka University

Principal Investigator

Nakatsuka Takashi  静岡大学, 農学部, 准教授 (60435576)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywordsストック / アントシアニン / RNA-seq / 遺伝子変異 / ゲノム解析 / ウイルスベクター / 形質転換 / 次世代シークエンサー / DNA多型 / 遺伝子組換え / 次世代シークエンス / 花色 / 花粉致死 / 白花 / 多型解析 / 多型 / 八重咲き / 花卉園芸
Outline of Final Research Achievements

Garden stock (Matthiola incana) is one of the most popular floricultural plants in winter season. We performed RNA-seq analysis using the petals of Vintage Lavender, and were successful to identify the anthocyanin biosynthetic structural and regulator genes . In addition, we revealed that the levels of bHLH2 transcripts were correlated well with those of anthocyanin accumulation, and that the deficiencies of either ans or bhlh1 were responsible for white flower coloration. TuMV vector is good tool to analysis gene function in stock. Moreover, we were also successful to produce stable transgenic stock plants.

Academic Significance and Societal Importance of the Research Achievements

ストックは冬季に栽培しやすい品目であり、その品種開発については日本がトップレベルである。これまでストックの品種開発は交雑育種によって行われてきた。主要な作物や野菜では遺伝子やゲノム情報が育種に活用されている。新たな形質を持ったストック品種の作出のためには遺伝子やゲノム情報の整備が必要不可欠であった。本研究成果によりストック遺伝子情報や機能解析ツールが整備され、今後ストック育種が促進されることが期待できる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (14 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (6 results) Remarks (1 results)

  • [Int'l Joint Research] IBMCP-CSIC(スペイン)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] IBMCP-CSIC(スペイン)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Two Independent Spontaneous Mutations Related to Anthocyanin-less Flower Coloration in <i>Matthiola incana</i> Cultivars2021

    • Author(s)
      Nuraini Latifa、Tatsuzawa Fumi、Ochiai Masaki、Suzuki Katsumi、Nakatsuka Takashi
    • Journal Title

      The Horticulture Journal

      Volume: 90 Issue: 1 Pages: 85-96

    • DOI

      10.2503/hortj.UTD-212

    • NAID

      130007975542

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anthocyanin regulatory and structural genes associated with violet flower color of Matthiola incana2020

    • Author(s)
      Nuraini Latifa、Ando Yukiko、Kawai Kentaro、Tatsuzawa Fumi、Tanaka Kotomi、Ochiai Masaki、Suzuki Katsumi、Aragones Veronica、Daros Jose-Antonio、Nakatsuka Takashi
    • Journal Title

      Planta

      Volume: 251 Issue: 3 Pages: 61-61

    • DOI

      10.1007/s00425-020-03351-z

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Floral organ- and temperature-dependent regulation of anthocyanin biosynthesis in Cymbidium hybrid flowers2019

    • Author(s)
      Nakatsuka T, Suzuki T, Harada K, Kobayashi Y, Dohra H, Ohno H.
    • Journal Title

      Plant Science

      Volume: 287 Pages: 110173-110173

    • DOI

      10.1016/j.plantsci.2019.110173

    • NAID

      120006707447

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of post-transcriptional gene silencing of chalcone synthase genes in petunia picotee petals by fluacrypyrim2019

    • Author(s)
      Ban Yusuke、Morita Yasumasa、Ogawa Mika、Higashi Katsumi、Nakatsuka Takashi、Nishihara Masahiro、Nakayama Masayoshi
    • Journal Title

      Journal of Experimental Botany

      Volume: 70 Issue: 5 Pages: 1513-1523

    • DOI

      10.1093/jxb/erz009

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of a double-flower mutation in Matthiola incana2018

    • Author(s)
      Nakatsuka Takashi, Koishi Kanae
    • Journal Title

      Plant Science

      Volume: 268 Pages: 39-46

    • DOI

      10.1016/j.plantsci.2017.12.009

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] ストック品種の白花化に関与した変異遺伝子の特定2021

    • Author(s)
      中塚貴司
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ストック花色に関わるアントシアニン生合成遺伝子の特定2020

    • Author(s)
      中塚貴司・Latifa Nuraini・安藤有季子
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ストック花色に関わるアントシアニン生合成遺伝子の特定.2020

    • Author(s)
      中塚貴司・Latifa Nuraini・安藤有季子
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ストックにおけるカブモザイクウイルスベク ターによる一過的遺伝子発現2019

    • Author(s)
      安藤有季子・河合健太郎・田中琴巳・Aragones Veronica・Daros Jose-Antonio・中塚 貴司
    • Organizer
      第37回日本植物細胞分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification of anthocyanin biosynthetic regulatory genes in Matthiola incana2019

    • Author(s)
      Latifa Nuraini, Yukiko Ando, Kentaro Kawai, Veronica Aragones, Jose-Antonio Daros and Takashi Nakatsuka
    • Organizer
      第37回日本植物細胞分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ストックにおける形質転換法の検討2017

    • Author(s)
      河合健太郎・中塚貴司
    • Organizer
      園芸学会平成29年度春季大会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 静岡大学農学部生物資源科学科花卉園芸学研究室

    • URL

      https://sites.google.com/site/shizuokaflower/home

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report 2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi